水稻香味基因及其在育种中的应用研究进展

doi:10.11983/CBB16197

植物学报 ›› 2017, Vol. 52 ›› Issue (6): 797-807.DOI: 10.11983/CBB16197

水稻香味基因及其在育种中的应用研究进展

彭波^1,^2,^*(), 孙艳芳^1,², 陈报阳^1,², 孙瑞萌^1,², 孔冬艳^1,², 庞瑞华^1,², 李先文^1,², 宋晓华³, 李慧龙³, 李金涛^1,², 周棋赢^1,², 柳琳⁴, 段斌³, 宋世枝^3,^*()

¹信阳师范学院生命科学学院, 信阳 464000
²信阳师范学院大别山农业生物资源保护与利用研究院, 信阳 464000
³信阳市农业科学院, 信阳 464000
⁴信阳学院理工学院, 信阳 464000

收稿日期:2016-10-11 接受日期:2017-01-10 出版日期:2017-11-01 发布日期:2018-02-22
通讯作者: 彭波,宋世枝
基金资助:
国家自然科学基金(No.U1604110, No.31270727, No.U1404319, No.31600992)、河南省重点科技攻关项目(No.15210211 0100, No.152102110036)、河南省高等学校重点科研项目(No.15A180059, No.16B180006)、河南省重大科技专项(No.121100110200)、河南省成果转化项目(No.142201110038)、河南省科技惠民项目(No.162207310009)、河南省高校科技创新团队(No.14IRTSTHN012)、信阳师范学院高层次人才科研启动基金(No.0201430)、河南省高等学校大学生实践创新训练计划(No.201710477018Y)、信阳师范学院“南湖学者奖励计划”青年项目(No.2016056)和大别山农业生物研究院开放课题

Research Progress of Fragrance Gene and Its Application in Rice Breeding

Peng Bo^1,^2,^*(), Sun Yanfang^1,², Chen Baoyang^1,², Sun Ruimeng^1,², Kong Dongyan^1,², Pang Ruihua^1,², Li Xianwen^1,², Song Xiaohua³, Li Huilong³, Li Jintao^1,², Zhou Qiying^1,², Liu Lin⁴, Duan Bin³, , Song Shizhi^3,^*()

¹College of Life Sciences, Xinyang Normal University, Xinyang 464000, China
²Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China
³Xinyang Academy of Agricultural Sciences, Xinyang 464000, China
⁴School of Sciences and Technology, Xinyang University, Xinyang 464000, China

Received:2016-10-11 Accepted:2017-01-10 Online:2017-11-01 Published:2018-02-22
Contact: Peng Bo,Song Shizhi

摘要/Abstract

摘要： 水稻(Oryza sativa)为世界上30多亿人口的主食, 是最重要的粮食作物之一。作为栽培水稻类型之一的香稻, 由于其稻米具有独特的香味, 在国内外市场上深受广大消费者的青睐。近年来, 随着水稻功能基因组和测序技术的快速发展, 针对水稻香味基因的研究取得了较大进展, 并开发了一系列的功能标记应用于香味基因筛选和品种培育。该文综述了水稻香味基因的遗传基础、基因功能及其调控、功能标记的开发及应用的新进展, 以期为香稻新品种培育提供借鉴与参考。

关键词: 水稻, 香味基因, 应用, 遗传育种

Abstract: Rice is a staple food for more than 3 billion people in the world, and it is one of the most important food crops. As one of the types of cultivated rice, fragrant rice is favoured worldwide by consumers because of its agreeable scent. In recent years, with the rapid development of rice functional genomics and sequencing technology, great progress has been made in understanding the fragrant gene in rice, and a series of functional markers has been developed for screening the fragrant gene and breeding new rice varieties. This paper mainly reviews the progress in the genetic basis, gene function and regulation of the fragrant gene and the development and application of gene functional markers in rice. It provides useful references for the cultivation of new varieties of fragrant rice.

Key words: rice, fragrance gene, application, genetic breeding

彭波, 孙艳芳, 陈报阳, 孙瑞萌, 孔冬艳, 庞瑞华, 李先文, 宋晓华, 李慧龙, 李金涛, 周棋赢, 柳琳, 段斌, 宋世枝. 水稻香味基因及其在育种中的应用研究进展. 植物学报, 2017, 52(6): 797-807.

Peng Bo, Sun Yanfang, Chen Baoyang, Sun Ruimeng, Kong Dongyan, Pang Ruihua, Li Xianwen, Song Xiaohua, Li Huilong, Li Jintao, Zhou Qiying, Liu Lin, Duan Bin, , Song Shizhi. Research Progress of Fragrance Gene and Its Application in Rice Breeding. Chinese Bulletin of Botany, 2017, 52(6): 797-807.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB16197

https://www.chinbullbotany.com/CN/Y2017/V52/I6/797

图/表 2

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功能标记	引物序列(5'-3')	位置	退火温度(°C)	PCR产物大小(bp) (非香/香)
FMU1-2	F: TCCCACCACCACTCCACA	5′UTR	61	163/160
	R: ACGAAGAGCTGCCGCTGC
FME2-7	F: ACGAAGAGCTGCCGCTGC	第2外显子	61	78/71
	R: GCGATTGCGCGGAGGTACT
FME7	F: TCCTGTAATCATGTATACCC	第7外显子	50	151/143
	R: AATTTGGAAACAAACCTT
FME12-3	F: TTGGTCCAGTGCTCTGTGTG	第12外显子	58	192/189
	R: GCACCAGCCAGACCATAAC
FME13	F: TTGGTCCAGTGCTCTGTGTG	第13外显子	58	192/195
	R: GCACCAGCCAGACCATAAC
FME14	F: TCGATGCCGGAATTATCTGGGTGA	第14外显子	61	60, 205/266
	R: TCCCCACGGCTCATCGGAGG

功能标记	引物序列(5'-3')	位置	退火温度(°C)	PCR产物大小(bp) (非香/香)
FMU1-2	F: TCCCACCACCACTCCACA	5′UTR	61	163/160
	R: ACGAAGAGCTGCCGCTGC
FME2-7	F: ACGAAGAGCTGCCGCTGC	第2外显子	61	78/71
	R: GCGATTGCGCGGAGGTACT
FME7	F: TCCTGTAATCATGTATACCC	第7外显子	50	151/143
	R: AATTTGGAAACAAACCTT
FME12-3	F: TTGGTCCAGTGCTCTGTGTG	第12外显子	58	192/189
	R: GCACCAGCCAGACCATAAC
FME13	F: TTGGTCCAGTGCTCTGTGTG	第13外显子	58	192/195
	R: GCACCAGCCAGACCATAAC
FME14	F: TCGATGCCGGAATTATCTGGGTGA	第14外显子	61	60, 205/266
	R: TCCCCACGGCTCATCGGAGG

水稻香味基因及其在育种中的应用研究进展

Research Progress of Fragrance Gene and Its Application in Rice Breeding

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