水稻窄卷叶突变体nrl7的鉴定与基因定位

doi:10.11983/CBB15205

植物学报 ›› 2016, Vol. 51 ›› Issue (3): 290-295.DOI: 10.11983/CBB15205 cstr: 32102.14.CBB15205

水稻窄卷叶突变体nrl7的鉴定与基因定位

王伟, 王嘉宇^*, 杨生龙, 刘进, 董晓雁, 王国骄, 陈温福

沈阳农业大学水稻研究所, 东北粳稻遗传改良与优质高效生产协同创新中心, 沈阳 110866

收稿日期:2015-11-16 修回日期:2016-01-25 出版日期:2016-05-01 发布日期:2016-05-24
通讯作者: 王嘉宇
基金资助:
教育部长江学者和创新团队发展计划(No.IRT13079)与国家自然科学基金(No.31201140)

Identification and Gene Mapping of the nrl7 Mutant in Rice

Wei Wang, Jiayu Wang^*, Shenglong Yang, Jin Liu, Xiaoyan Dong, Guojiao Wang, Wenfu Chen

Collaborative Innovation Center for Genetic Improvement and Quality and Efficient Production of Japonica Rice in the Northeast of China, Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, China

Received:2015-11-16 Revised:2016-01-25 Online:2016-05-01 Published:2016-05-24
Contact: Jiayu Wang

摘要/Abstract

摘要： 叶片的形态是理想株型的重要性状, 叶片适度卷曲能提高水稻(Oryza sativa)群体的光能利用率, 研究控制水稻叶片形态的相关基因能够进一步丰富株型理论。该研究在粳稻品系C275的群体中发现了1株自然变异的窄卷叶突变体nrl7(narrow rolled leaf 7)。与野生型相比, 突变体的叶片变窄且向内卷曲; 该突变体叶片连接中脉的泡状细胞严重变形, 中脉与小叶脉之间的维管束数量均减少至1个。此外, 突变体nrl7的株高、实粒数和实粒重均降低或减少, 分别为野生型的88.46%、69.77%和68.98%, 差异达极显著水平。叶片卷曲导致单叶光合速率减弱, 与野生型相比, 突变体的光合速率降低了17%, 达极显著水平。突变体nrI7叶片的气孔导度、胞间CO₂浓度和蒸腾速率则与野生型相比无明显变化。利用图位克隆的方法将目的基因定位于水稻第3染色体短臂上的分子标记RM5444和MM1300之间, 物理距离约为185.14 kb。研究结果为该基因的克隆和进一步的功能分析奠定了基础。

关键词: 水稻, 窄卷叶突变体, 基因定位

Abstract: Leaf morphology is an important trait of ideotype breeding; moderate rolling of leaves can enhance light-use efficiency. Study of genes that control leaf morphology can enrich the theory of ideal plant architecture in rice. We found a novel spontaneous mutant nrl7 with narrow rolled leaves in the japonica C275 population that can be stably inherited. Compared to the wild type, the leaves of nrl7 narrowed and rolled inward, the number of vascular bundles between the leaf midrib and the adjacent vein was reduced to one, and the bulliform cells showed significant morphological change. Nevertheless, the plant height, filled grains per panicle, and filled grain weight per panicle in the mutant were 88.46%, 69.77%, 68.98%, respectively, of that in the wild type. Photosynthetic rate was significantly higher in the mutant than the wild type and accounted for 17% of that in C275. Transpiration rate did not differ. Map-based cloning revealed NRL7 on chromosome 3 between markers RM5444 and MM1300, delimited to a 185.14 kb region. These results will lay a good
foundation for molecular cloning and functional analysis of NRL7.

Key words: rice, narrow rolled leaf mutant, gene mapping

王伟, 王嘉宇, 杨生龙, 刘进, 董晓雁, 王国骄, 陈温福. 水稻窄卷叶突变体nrl7的鉴定与基因定位. 植物学报, 2016, 51(3): 290-295.

Wei Wang, Jiayu Wang, Shenglong Yang, Jin Liu, Xiaoyan Dong, Guojiao Wang, Wenfu Chen. Identification and Gene Mapping of the nrl7 Mutant in Rice. Chinese Bulletin of Botany, 2016, 51(3): 290-295.

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https://www.chinbullbotany.com/CN/Y2016/V51/I3/290

参考文献

[1] 郎有忠, 张祖建, 顾兴友, 等. 水稻卷叶性状生理生态效应的研究Ⅰ. 姿态、群体构成及广分布特征［J］. 作物学报, 2004, 30（8）: 806-810
[2] 陆江锋, 郎有忠, 张祖建, 等. 水稻一组卷叶近等基因系的株形、群体结构和光合特性比较［J］. 扬州大学学报: 农业与生命科学版, 2005, 6（2）: 56-60
[3] 陈宗祥, 潘学彪, 胡俊. 水稻卷叶性状及理想株型的关系［J］. 江苏农业研究, 2001, 22(4): 88-91
[4] 沈福成. 关于水稻卷叶性状在育种中利用的几点看法［J］. 贵州农业科学, 1983, (5): 6-8
[5] 陈代波, 程式华, 曹立勇. 水稻窄叶性状的研究进展［J］. 中国稻米, 2010, 16（3）: 1-4
[6] 余东, 吴海滨, 杨文韬, 等. 水稻单侧卷叶突变体B157遗传分析及基因初步定位［J］. 分子植物育种, 2008, 6（2）: 220-226
[7] 曾生元, 郭旻, 李敏, 等. 个水稻动态窄叶突变体的鉴定和基因定位［J］. 科学通报, 2010, 55(21): 210-2111
[8] 潘存红, 李磊, 陈宗祥, 等.一个水稻卷叶基因 rl(t)的精细定位［J］.中国水稻科学, 2011, 25（5）: 455-460
[9] Zou L P, Sun X H, hang Z G, et al. Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice［J］. Plant Physiology, 2011,156 ( 3 ) : 1589-1602
[10] 王德仲, 桑贤春, 游小庆, 等. 水稻细卷叶突变体 nrl2(t)的遗传分析和基因定位［J］. 作物学报, 2011, 37（7）: 1159-1166
[11] Shi Z Y, Wang J, Wan X S, et al. Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit［J］. Planta, 2007, 226 (1) : 99-108
[12] Fujino K, Matsuda Y, Ozawa K, et al. NARROW LEAF 7controls leaf shape mediated by auxin in rice［J］. Molecular Genetics Genomics: MGG, 2008, 279(5): 499-507
[13] Qi J, Qian Q, Bu Q Y, et al. Mutation of the rice Narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport［J］. Plant Physiology, 2008, 147(4): 1947-195
[14] 李仕贵, 马玉清, 何平, 等. 一个未知的卷叶基因的识别和定位［J］. 四川农业大学学报, 1998, 16(4): 391-393
[15] 邵元健, 陈宗祥, 张亚芳, 等. 一个水稻卷叶主效 QTL的定位及其物理图谱的构建［J］. 遗传学报, 2005, 32(5): 501-506
[16] Xiang J J, Zhang G H, Qian Q, et al. SEMI-ROLLED LEAF1 encodes a putative glycosyl phosphatidylinositol-anchored protein and modulates rice leaf rolling by regulating the formation of bulliform cells［J］. Plant Physiology, 2012, 159(4): 1488-1500
[17] Shi Y F, Chen J, Liu W Q, et al. Genetic analysis and gene mapping of a new rolled-leaf mutant in rice( Oryza sativa L.) ［J］. Science in China Series C: Life Sciences, 2009, 52(9): 885-890
[18] Yan C J, Yan S, Zhang Z Q, et al.Genetic analysis and gene fine mapping for a rice novel mutant(rl9(t)) with rolling leaf character［J］. Chinese Science Bulletin,2006, 51(1): 63-69
[19] 罗远章, 赵芳明, 桑贤春, 等. 水稻新型卷叶突变体rl12(t)的遗传分析和基因定［J］. 作物学报, 2009, 35(11): 1967-1972
[20] Fang L K, Zhao F M, Cong Y F, et al. Rolling-leaf14 is a 2OG-Fe (Ⅱ) oxygenase family protein that modulates rice leaf rolling by affecting secondary cell wall formation in leaves［J］. Plant Biotechnology Journal, 2012, 10(5): 524-532
[21] Cho S H, Yoo S C, Zhang H T, et al. The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A( OsWOX3A) and function in leaf, spikelet, tiller and lateral root development［J］. New Phytologist, 2013, 198(4): 1071-1084
[22] Hu J, Zhu L, Zeng D L, et al. Identification and characterization of NARROW and ROLLED LEAF1, a novel gene regulating leaf morphology and plant architecture in rice［J］. Plant Molecular Biology, 2010, 73(3): 283-292
[23] 高艳红, 吕川根, 王茂青, 等. 水稻卷叶性状QTL的初步定位［J］. 江苏农业学报, 2007, 23(1): 5-10
[24] 李和平. 植物显微技术[M]. 北京: 科学出版社, 2009: 9-48
[25] Rogers S O, Bendich A J. Extraction of DNA from plant tissues［J］. Plant Mol Biol Manual, 1989, pp73-83
[26] Scarpella E, Barkoulas M, Tsiantis M. Control of leaf and vein development by auxin［J］. Cold Spring Harb Perspect Biol, 2010, 2(1): a001511
[27] Dettmer J, Elo A, Helariutta Y. Hormone interactions during vascular development［J］. Plant Mol Biol, 2009, 69(4): 347-360
[28] Micol L J, Hake S. The development of plant leaves［J］. Plant Physiol, 2003, 131(2): 389-394
[29] 严松, 严长杰, 顾铭洪. 植物叶发育的分子机理［J］. 遗传, 2008, 30(9): 1127-1135
[30] Shao Y J, Pan C H, Chen Z X, et al. Fine mapping of an incomplete recessive gene for leaf rolling in rice (Oryza sativa L.) ［J］. Chin Sci Bull, 2005, 50(21): 2466-2472
[31] Zhang G H, Xu Q, Zhu X D, et al. SHALLOTLIKE1 is a KANADI transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development［J］. Plant Cell, 2009, 2(3): 719-735
[32] Zhang, Z.H., Deng, Y.J., Tan, J., et al. A genome-wide microsatellite polymorphism database for the Indica and Japonica rice［J］. DNA Research, 2007, 14(1): 37-45
[33] 陈宗祥, 胡俊, 陈刚, 潘学彪. RL(t)卷叶基因对杂交稻经济性状的影响［J］.作物学报, 2004, 30(5): 465-69

水稻窄卷叶突变体nrl7的鉴定与基因定位

Identification and Gene Mapping of the nrl7 Mutant in Rice

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