植物学报 ›› 2019, Vol. 54 ›› Issue (6): 711-722.DOI: 10.11983/CBB19042
张彤1,郭亚璐1,2,陈悦1,马金姣1,兰金苹1,3,燕高伟1,刘玉晴1,徐珊1,李莉云1,刘国振1,*(),窦世娟1,*()
收稿日期:
2019-02-27
接受日期:
2019-05-06
出版日期:
2019-11-01
发布日期:
2020-07-09
通讯作者:
刘国振,窦世娟
基金资助:
Tong Zhang1,Yalu Guo1,2,Yue Chen1,Jinjiao Ma1,Jinping Lan1,3,Gaowei Yan1,Yuqing Liu1,Shan Xu1,Liyun Li1,Guozhen Liu1,*(),Shijuan Dou1,*()
Received:
2019-02-27
Accepted:
2019-05-06
Online:
2019-11-01
Published:
2020-07-09
Contact:
Guozhen Liu,Shijuan Dou
摘要: 利用免疫印迹(WB)分析了水稻(Oryza sativa) OsPR10A在其不同生长时期、不同组织部位及多种非生物逆境胁迫下的表达特征, 发现OsPR10A在干旱、盐胁迫以及茉莉酸甲酯(MeJA)和脱落酸(ABA)诱导下表达量明显升高, 表明该蛋白可能在干旱和盐胁迫应答过程中发挥作用。为证明这一推测, 我们构建了OsPR10A超表达载体, 经农杆菌介导转化水稻, 获得超表达OsPR10A的纯合株系。田间表型观察表明, 转基因株系株高变矮、穗长变短、结实率降低。用20% PEG6000在水稻种子萌发过程中进行干旱处理, 结果显示, OsPR10A超表达株系的根长和芽长均显著高于野生型, 证明超表达OsPR10A可增强水稻萌发期耐旱性。该研究有助于增进人们对水稻OsPR10A功能的了解。
张彤,郭亚璐,陈悦,马金姣,兰金苹,燕高伟,刘玉晴,徐珊,李莉云,刘国振,窦世娟. 水稻OsPR10A的表达特征及其在干旱胁迫应答过程中的功能. 植物学报, 2019, 54(6): 711-722.
Tong Zhang,Yalu Guo,Yue Chen,Jinjiao Ma,Jinping Lan,Gaowei Yan,Yuqing Liu,Shan Xu,Liyun Li,Guozhen Liu,Shijuan Dou. Expression Characterization of Rice OsPR10A and Its Function in Response to Drought Stress. Chinese Bulletin of Botany, 2019, 54(6): 711-722.
图1 OsPR10A在水稻生长发育过程中的表达 (A) OsPR10A在水稻不同生长时期和组织部位的表达(Sd: 苗期; Ti: 分蘖期; Bt: 孕穗期; Fw: 开花期); (B) OsPR10A在萌发期种子中的表达; (C) OsPR10A在开花期组织中的表达(Sp: 穗; Ss: 穗轴; An: 花药; Husk: 颖壳; Low: 叶下部; Mid: 叶中部; Up: 叶上部); (D) OsPR10A在幼苗和旗叶中的表达
Figure 1 Expression of OsPR10A during rice growth and development (A) Expression of OsPR10A in different growth stages and tissue parts of rice (Sd: Seedling stage; Ti: Tillering stage; Bt: Booting stage; Fw: Flowering stage); (B) Expression of OsPR10A in seeds during germination; (C) Expression of OsPR10A in tissues during flowering stage (Sp: Spike; Ss: Spike-stalk; An: Anther; Husk: Husk; Low: Lower part of leaf; Mid: Middle part of leaf; Up: Upper part of leaf); (D) Expression of OsPR10A in seedling and flag leaf
Library description | FPKM |
---|---|
Shoots | 50.1661 |
Leaves-20 day | 144.7290 |
Pre-emergence inflorescence | 4.1989 |
Post-emergence inflorescence | 88.9886 |
Anther | 0.5100 |
Pistil | 2.3483 |
Seed-5 DAP | 77.4876 |
Seed-10 DAP | 0.0000 |
Embryo-25 DAP | 1.5052 |
Endosperm-25 DAP | 2.3371 |
表1 OsPR10A基因的转录分析
Table 1 Transcriptome analysis of the OsPR10A gene
Library description | FPKM |
---|---|
Shoots | 50.1661 |
Leaves-20 day | 144.7290 |
Pre-emergence inflorescence | 4.1989 |
Post-emergence inflorescence | 88.9886 |
Anther | 0.5100 |
Pistil | 2.3483 |
Seed-5 DAP | 77.4876 |
Seed-10 DAP | 0.0000 |
Embryo-25 DAP | 1.5052 |
Endosperm-25 DAP | 2.3371 |
图2 水稻OsPR10A在非生物胁迫下的动态表达 (A) OsPR10A在干旱胁迫下的表达; (B) OsPR10A在盐胁迫下的表达; (C) OsPR10A在外源施加茉莉酸甲酯后的表达; (D) OsPR10A在外源施加脱落酸后的表达。MeJA: 茉莉酸甲酯; ABA: 脱落酸
Figure 2 Dynamic expression of rice OsPR10A under abiotic stress (A) Expression of OsPR10A under drought stress; (B) Expression of OsPR10A under salt stress; (C) Expression of OsPR10A modulated by exogenous MeJA; (D) Expression of OsPR10A modulated by exogenous ABA. MeJA: Methyl jasmonate; ABA: Abscisic acid
图3 超表达OsPR10A转基因水稻的鉴定 A721、A726、A728和A730分别代表不同转基因株系; 泳道1-7或1-8分别代表同一转基因株系的不同植株; WT: 野生型
Figure 3 Identification of OsPR10A overexpression transgenic rice A721, A726, A728 and A730 represent different transgenic lines, respectively; Lane 1-7 or 1-8 represent different plants of the same transgenic line, respectively; WT: Wildtype
图4 超表达OsPR10A转基因水稻的表型和农艺性状 (A) 成熟期植株(Bar=10 cm); (B) 穗部(Bar=4 cm); (C)-(F) 分别表示株高、穗长、结实率和分蘖数。WT: 野生型。* P<0.05; ** P<0.01
Figure 4 Phenotype of OsPR10A overexpression transgenic rice (A) Mature plant (Bar=10 cm); (B) Spike (Bar=4 cm); (C)-(F) Represent plant height, spike length, seed setting rate and tiller number, respectively. WT: Wildtype. * P<0.05; ** P<0.01
图5 超表达OsPR10A增强水稻种子萌发期的耐旱性 (A) 干旱处理(20% PEG6000) 7天萌发后的水稻种子(Bar=1 cm); (B) 根长; (C) 芽长。WT: 野生型。* P<0.05; ** P<0.01
Figure 5 Overexpression of OsPR10A enhanced the drought tolerance at rice seed germination stage (A) The germinated rice seeds at 7 days treated with 20% PEG6000 (Bar=1 cm); (B) Root length; (C) Shoot length. WT: Wildtype. * P<0.05; ** P<0.01
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