植物学报 ›› 2019, Vol. 54 ›› Issue (4): 503-508.doi: 10.11983/CBB19087

• 技术方法 • 上一篇    下一篇

水稻减数分裂染色体分析方法

程新杰1,于恒秀1,程祝宽2,*()   

  1. 1 扬州大学农学院, 扬州 225009
    2 中国科学院遗传与发育生物学研究所植物基因研究中心, 北京 100101
  • 收稿日期:2019-05-12 接受日期:2019-06-14 出版日期:2019-07-01 发布日期:2019-07-01
  • 通讯作者: 程祝宽 E-mail:zkcheng@genetics.ac.cn

Protocols for Analyzing Rice Meiotic Chromosomes

Cheng Xinjie1,Yu Hengxiu1,Cheng Zhukuan2,*()   

  1. 1 Agriculture College, Yangzhou University, Yangzhou 225009, China
    2 Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2019-05-12 Accepted:2019-06-14 Online:2019-07-01 Published:2019-07-01
  • Contact: Cheng Zhukuan E-mail:zkcheng@genetics.ac.cn

摘要:

减数分裂粗线期染色体研究技术的发展, 很大程度上克服了水稻(Oryza sativa)细胞遗传研究中较小染色体所带来的研究困难。减数分裂染色体的制备与观察已经成为水稻细胞遗传学研究中的常规方法。该文详细描述了水稻中常用的减数分裂染色体制备、荧光原位杂交和免疫荧光染色的实验方法。

关键词: 水稻, 减数分裂, 染色体, 荧光原位杂交, 免疫荧光染色

Abstract:

The development of techniques to analyze pachytene chromosomes has greatly overcome most of the difficulties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies of this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis and immunocytology in rice.

Key words: rice, meiosis, chromosome, FISH, immunostaining

图1

水稻减数分裂粗线期染色体 (A) 水稻花粉母细胞减数分裂粗线期染色体的荧光原位杂交结果, 红色为端粒探针pAtT4的杂交信号, 蓝色为DAPI染色的粗线期染色体(普通荧光显微镜下观察); (B) 水稻花粉母细胞减数分裂粗线期染色体的免疫荧光染色结果, 绿色为PAIR3荧光信号, 红色为ZEP1荧光信号(超分辨荧光显微镜下观察)。Bars=5 µm"

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