Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya

doi:10.11983/CBB24051

Abstract

Abstract: To establish a tissue culture regeneration system for cultivated Chinese yam (Dioscorea polystachya), the effects of different concentrations of plant growth regulators, and stem segments on axillary bud induction and plant regeneration were investigated with different concentrations of plant growth regulators, media types, and shoot tips and stem segments at four different positions under the shoot tip as explants. The results revealed that MS+1.0 mg∙L^-1 6-BA+0.5 mg∙L^-1 KT hormone ratio and 12-20 cm stem segment under the stem tip were the best formulas for the induction of adventitious buds in D. polystachya, and the induction rate reached 90.0%. In the subculture, the optimal concentration was MS+0.5 mg∙L^-1 6-BA+0.05 mg∙L^-1 NAA+0.1 mg∙L^-1 KT, and the proliferation coefficient increased with increasing of 6-BA within a certain range when the low concentration of NAA was constant. With the same concentration of exogenous hormones, DKW medium was significantly better than the conventional 1/2MS medium, and the rooting rate is significantly improved, reaching 92.86%. Adding 1 mg PVP (polyvinylpyrrolidone) and increasing the number of transfers can significantly reduce browning. This study effectively solved the problem of optimizing the explant position for the stable of in vitro propagation of cultivated yam germplasm resources, laying a good foundation for the large-scale production of their high-quality virus-free seedlings.

Key words: Dioscorea polystachya, stem segments, tissue culture, in vivo regeneration

Wen Feng, Yuguo Wang. Establishment of an In Vitro Regeneration System for Stem Segments of Cultivated Dioscorea polystachya[J]. Chinese Bulletin of Botany, 2024, 59(5): 792-799.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24051

https://www.chinbullbotany.com/EN/Y2024/V59/I5/792

Figures/Tables 4

References 38

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No.	Length of stem segment (cm)	6-BA (mg∙L^-1)	KT (mg∙L^-1)	Induction rate (%)	Death rate (%)	Pollution rate (%)	Growth condition
1	Stem tip	1.0	0.5	11.1	88.9	0	+
2	2-5	1.0	0.5	68.4	15.8	5.3	+++
3	5-12	1.0	0.5	88.6	6.8	2.3	+++
4	5-12	2.0	0.5	54.1	4.2	4.2	+++
5	12-20	1.0	0.5	90.0	10.0	0	+++++
6	12-20	2.0	0.5	66.7	26.7	6.7	++++
7	12-20	3.0	0.5	60.0	26.7	13.3	+++
8	>20	3.0	0.5	75.0	6.3	18.8	+++

No.	Length of stem segment (cm)	6-BA (mg∙L^-1)	KT (mg∙L^-1)	Induction rate (%)	Death rate (%)	Pollution rate (%)	Growth condition
1	Stem tip	1.0	0.5	11.1	88.9	0	+
2	2-5	1.0	0.5	68.4	15.8	5.3	+++
3	5-12	1.0	0.5	88.6	6.8	2.3	+++
4	5-12	2.0	0.5	54.1	4.2	4.2	+++
5	12-20	1.0	0.5	90.0	10.0	0	+++++
6	12-20	2.0	0.5	66.7	26.7	6.7	++++
7	12-20	3.0	0.5	60.0	26.7	13.3	+++
8	>20	3.0	0.5	75.0	6.3	18.8	+++

No.	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	KT (mg∙L^-1)	Subculture numbers	Total bud numbers	Propagation coefficient	Growth condition
1	2.0	0.2	0	70	Low	Low	+
2	2.0	0.3	1.0	70	Low	Low	+
3	0.5	0.05	0.1	70	140	2.0	++++
4	0.5	0.05	0	70	121	1.7	+++
5	0.3	0.05	0	70	105	1.5	++
6	0.2	0.05	0	70	98	1.4	++

No.	6-BA (mg∙L^-1)	NAA (mg∙L^-1)	KT (mg∙L^-1)	Subculture numbers	Total bud numbers	Propagation coefficient	Growth condition
1	2.0	0.2	0	70	Low	Low	+
2	2.0	0.3	1.0	70	Low	Low	+
3	0.5	0.05	0.1	70	140	2.0	++++
4	0.5	0.05	0	70	121	1.7	+++
5	0.3	0.05	0	70	105	1.5	++
6	0.2	0.05	0	70	98	1.4	++

No.	Different media	IBA (mg∙L^-1)	NAA (mg∙L^-1)	Active charcoal (g∙L^-1)	Rooting rate (%)	Growth condition
1	1/2MS	0.1	0.3	0.2	38.00	+
2	1/2MS	0.3	0.1	0.2	64.00	++
3	1/2MS	1.0	0.05	0	58.00	++
4	DKW	1.0	0.05	0	92.00	++++