Chinese Bulletin of Botany ›› 2010, Vol. 45 ›› Issue (05): 548-555.DOI: 10.3969/j.issn.1674-3466.2010.05.004
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Qiuping Liu1,4, Hua Cao2,4, Maojin Yao3,4, Ying Ma3,4, Binsheng Deng3,4, Junli Quan4, Weixing Shan1,4*
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Abstract: Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants. A collection of more than 40 000 T3 A. thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P. sojae zoospores, and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization. A number of P. sojae-susceptible A. thaliana mutants were successfully obtained, and one of them, mutant 581-51, was shown to be stably susceptible to P. sojae infection. Water-soaked lesions formed on the detached leaves within 3 days, as did oospores and sporangia 4–5 days after inoculation with P. sojae zoospores. Cytological characterization revealed the formation of haustoria-like structures. Southern analysis showed the presence of four T-DNA insertion events in the mutant. Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P. sojae in the mutant 581-51 was likely controlled by a single recessive gene.
Qiuping Liu, Hua Cao, Maojin Yao, Ying Ma, Binsheng Deng, Junli Quan, Weixing Shan. Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant[J]. Chinese Bulletin of Botany, 2010, 45(05): 548-555.
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URL: https://www.chinbullbotany.com/EN/10.3969/j.issn.1674-3466.2010.05.004
https://www.chinbullbotany.com/EN/Y2010/V45/I05/548