Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 356-358.DOI: 10.11983/CBB23072

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The Broad-spectrum Innate Resistance Against Clubroot Disease Conferred by WeiTsing is Mechanistically Revealed

Gang Wang, Ertao Wang()   

  1. CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai 200032, China
  • Received:2023-05-31 Accepted:2023-06-03 Online:2023-05-01 Published:2023-06-08
  • Contact: *E-mail:

Abstract: Clubroot, Plasmodiophora brassicae (Pb) caused devastating disease, results in severe yield losses on cruciferous crops worldwide. Recently, Yu-hang Chen, Jian-Min Zhou and their colleagues from the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences reported the isolation and characterization of WeiTsing (WTS), a broad-spectrum clubroot resistance gene from Arabidopsis. WTS in Arabidopsisis was transcriptionally activated in the pericycle upon Pb infection to prevent pathogen colonization in the stele. WTS encodes a small protein that is localized in the endoplasmic reticulum (ER). The cryo-EM structure analysis has revealed a pentameric architecture of WeiTsing with a central pore, which was previously unknown. Electrophysiological analysis has demonstrated that WeiTsing functions as a calcium-permeable cation-selective channel. The Pb-mediated activation of the WTS channels induces immune responses, including cell death. Brassica napus plants carrying the WTS transgene have exhibited robust resistance to Pb. These findings have identified a novel ion channel similar to resistosomes, which triggers immune signaling in the pericycle. This discovery provides a valuable tool for the development of elite crop varieties.

Key words: clubroot, pericycle, Arabidopsis thaliana, Brassica napus, calcium channel, resistance