Identification of XTH Family Genes in Antirrhinum majus and Screening of Genes Involoved in Sclerotinia sclerotiorum Resistance and Stamen Petalization

doi:10.11983/CBB23107

Abstract

Abstract:

Xyloglucan endotransglucosylase/hydrolases (XTH) belongs to glycoside hydrolase, 16 family (GH16) and is a class of enzymes that mediate the construction and recombination of xyglucan-cellulose skeleton. To explore the potential biological functions of XTH family genes in Antirrhinum majus. In this paper, bioinformatics analysis, RNA-seq analysis and qRT-PCR were used to investigate the expression levels of the family genes in the petalized and non-petalized stamens and disease-resistant materials. The results showed that the main conserved motif of 33 identified AmXTH genes was ExDxE, which was divided into 3 subgroups. Most of the cis-acting elements of AmXTH promoter are growth and development, disease resistance and stress resistance. RNA-seq and qRT-PCR revalidation finally unearthed four candidate genes (AmXTH3, 14, 18, and 33) and one candidate genes (AmXTH23) for positively mediated Sclerotinia sclerotiorum resistance. There were 12 positive AmXTH candidate genes (AmXTH1, 7, 9, 11, 21, 22, 23, 24, 26, 28, 29 and 33) and 2 negative AmXTH candidate genes (AmXTH15 and 31). Among them, AmXTH23 and 33 may play a role in both the resistance of A. majus to S. sclerotiorum resistance and stamen petalization. In this study, the candidate AmXTH genes involved in S. sclerotiorum resistance and stamen petalization of A. majus were preliminarily excavated, which laid a foundation for further revealing the gene function.

Key words: A. majus, Xyloglucan endotransglucosylase/hydrolases family, S. sclerotiorum resistance, stamen petalization, differential gene expression

Hanqian Zhao, Jiayi Song, Jie Yang, Yongjing Zhao, Wennian Xia, Weizhuo Gu, Zhongyi Wang, Nan Yang, Huizhen Hu. Identification of XTH Family Genes in Antirrhinum majus and Screening of Genes Involoved in Sclerotinia sclerotiorum Resistance and Stamen Petalization[J]. Chinese Bulletin of Botany, 2024, 59(2): 188-203.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB23107

https://www.chinbullbotany.com/EN/Y2024/V59/I2/188

References

[1]范天刚(2014).月季花器官发育基因 AGAMOUS 对低温导致花朵过度重瓣化的作用研究.河北农业大学硕士论文, 无:1-58. [2]贺哲 (2018).猕猴桃受葡萄座腔菌侵染后木葡聚糖内糖基转移／水解酶 (XTH) 基因的克隆及功能分析.江西农业大学硕士论文, 无:1-59. [3]贺振，徐小伟，张坤，秦朗，郭枭 (2022).一种植物抗病相关蛋白 NbXTH1 其编码基因和应用. 中国专利，CN202210672471.1. 2022-08-12..中国专利, 无:1-4. [4]贾鑫磊，何贝轩，郭丹丹，郭美丽(2018).膨胀素和木葡聚糖内转葡糖基酶水解酶基因的功能研究进展.植物生理学报, 54:1659-1668. [5]江俊峰(2022).人参 XTH 家族基因生物信息学分析及两个 PgXTH 基因的功能分析.吉林大学硕士论文, 无:1-69. [6]罗维平，陈少萍，龚衍熙(2018).金鱼草的繁殖与病虫害防治.中国花卉园艺, 8:23-25. [7]田亚然，范天刚，张钢，李永红(2016).低温引起月季花朵过度重瓣化关键基因的表达及分析.热带作物学报, 37:1147-1154. [8]王瑞生(2019).金鱼草 AmDEFH28 基因克隆和功能分析.安徽农业大学硕士论文, 无:1-67. [9]吴健，周永明，王幼平(2018).油菜与核盘菌互作分子机理研究进展.中国油料作物学报, 40:721-729. [10]徐天舒(2021).红皮病人参细胞壁组分变化及两个非典型 XTH 家族基因的功能探究.吉林大学硕士论文, 无:1-59. [11]赵雪惠，王庆杰，李晨，陈修德，肖伟，高东升，付喜玲(2018).桃转录因子家族生物信息学分析及芽萌发相关基因筛选.植物学报, 53:612-624. [12]Cardarelli M and Cecchetti V(2014).Auxin polar transport in stamen formation and development: how many actors? 5, 333..Front. Plant Sci., 5:333-333. [13]Dora S, Terrett OM, Sánchez-Rodríguez C(2022).Plant-microbe interactions in the apoplast: communication at the plant cell wall.Plant Cell., 无:1532-1550. [14]Guo XM, Stotz HU(2007).Defense against Sclerotinia sclerotiorum in Arabidopsis is dependent on jasmonic acid,salicylic acid,and ethylene signaling.Mol Plant Mirobe Interact., 20:1384-1395. [15]Han Y, Ban Q, Hou Y, Meng K, Suo J, Rao J(2016).Isolation and characterization of two persimmon xyloglucan endotransglycosylase/hydrolase (XTH) genes that have divergent functions in cell wall modification and fruit postharvest softening.Front. Plant Sci., 7:624-624. [16]Harada T, Torii Y, Morita S, Onodera R, Hara Y, Yokoyama R, Nishitani K, Satoh S(2010).Cloning,characterization,and expression of xyloglucan endotransglucosylasehydrolase and expansin genes associated with petal growth and development during carnation flower opening.J Exp Bot., 62:815-823. [17]Hu HZ, Tang YW, Wu J, Chen FZ, Yang YD, Pan XC, Dong X, Jin XD, Liu S, Du XZ(2021).Brassica napus mediator subunit16 induces BnMED25-and BnWRKY33-activated defense signaling to confer Sclerotinia sclerotiorum resistance.Front. Plant Sci. , 无:663536-663536. [18]Hu HZ, Zhang R, Feng SQ, Wang YM, Wang YT, Fan CF, Li Y, Liu ZY, Schneider R, Xia T, Ding S-Y, Persson S, Peng LC(2018).Three AtCesA6-like members enhance biomass production by distinctively promoting cell growth in Arabidopsis.Plant Biotechnol J., 16:976-988. [19]Hyodo H, Yamakawa S, Takeda Y, Tsuduki M, Yokota A, Nishitani K, Kohchi T(2003).Active gene expression of a xyloglucan endotransglucosylasehydrolase gene,XTH9,in inflorescence apices is related to cell elongation in Arabidopsis thaliana.Plant Mol Biol., 52:473-482. [20]Li Q, Hu AH, Dou WF, Qi JJ, Long Q, Zou XP, Lei TG, Yao LX, He YR, Chen SC(2019).Systematic analysis and functional validation of citrus XTH genes reveal the role of Csxth04 in citrus bacterial canker resistance and tolerance.Front. Plant Sci., 10:1109-1109. [21]Malinowski R, Fry SC, Zuzga S, Wi?niewska A, Godlewski M, Noyszewski A, Barczak?Brzy?ek A, Malepszy S, Filipecki M (2018).Developmental expression of the cucumber CsXTH1 and CsXTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli.Acta Physiologiae Plantarum, 40:1307-1307. [22]Marciniak K and Przedniczek K(2019).Comprehensive insight into gibberellin- and jasmonate-mediated stamen development.Genes, 10:811-811. [23]Miedes E, Suslov D, Vandenbussche F, Kenobi K, Ivakov A et al.(2013).Xyloglucan endotransglucosylasehydrolase (XTH) overexpression affects growth and cell wall mechanics in etiolated Arabidopsis hypocotyls.J Exp Bot., 无:2481-97. [24]Ochiai M, Matsumoto S, Maesaka M, Yamada K(2013).Expression of mRNAs and proteins associated with cell-wall-loosening during Eustoma flower opening.J. Jpn. Soc. Hortic. Sci., 82:154-160. [25]Pitaksaringkarn W, Matsuoka K, Asahina M, Miura K, Sage-Ono K, Ono M, Yokoyama R, Nishitani K, Ishii T, Iwai H, Satoh S(2014).XTH20 and XTH19 regulated by ANAC071 under auxin flow are involved in cell proliferation in incised Arabidopsis inflorescence stems.Plant J., 无:604-14. [26]Qiao T, Zhang L, Yu YY, Pang YN, Tang XJ, Wang X, Li LJ, Li B, Sun QH(2022).Identification and expression analysis of xyloglucan endotransglucosylase/ hydrolase (XTH) family in grapevine (Vitis vinifera L.).Peer J, 10:13546-13546. [27]Sasidharan R and Pierik R(2010).Cell wall modification involving XTHs controls phytochrome-mediated petiole elongation in Arabidopsis thaliana.Plant Signal Behav., 5:1491-1492. [28]Takahashi D, Johnson Kim L, Hao PF, Tuong T, Erban A, Sampathkumar A, Bacic A Livingston DP 3rd, Kopka J, Kuroha T, Yokoyama R, Nishitani K, Zuther E, Hincha DK(2021).Cell wall modification by the xyloglucan endotransglucosylasehydrolase XTH19 influences freezing tolerance after cold andsub-zero acclimation.Plant Cell Environ., 44:915-930. [29]Watanabe Y, Niki T, Norikoshi R, Nakano M, Ichimura Kn(2022).Soluble carbohydrate concentration and expression of expansin and xyloglucan endotransglucosylase /hydrolase genes in epidermal and parenchyma cells during lily flower opening.J Plant Physiol., 270:153615-153615. [30]Witasari LD, Huang FC, Hoffmann T, Rozhon W, Fry SC, Schwab W(2019).Higher expression of the strawberry xyloglucan endotransglucosylasehydrolase genes FvXTH9 and FvXTH6 accelerates fruit ripening.Plant J., 100:1237-1253. [31]Wu D, Liu AQ, Qu XY, Liang JY, Song M (2020).Genome-wide identification, and phylogenetic and expression profiling analyses of XTH gene families in Brassica rapa L. and Brassica oleracea L. .BMC Genomics, 21:782-782. [32]Xu P, Fang S, Chen H, Cai W(2020).The brassinosteroid-responsive xyloglucan endotransglucosylasehydrolase 19 (XTH19) and XTH23 genes are involved in lateral root development under salt stress in Arabidopsis.Plant J., 无:59-75. [33]Yang Y, Miao YF, Zhong SW, Fang Q, Wang YG, Dong B, Zhao HB(2022).Genome-wide identification and expression analysis of XTH gene family during flower-opening stages in Osmanthus fragrans.Plants, 11:1015-1015. [34]Zhang R, Hu Z, Wang YT, Hu HZ, Li FC, Li M, Ragauskas A, Xia T, Han HY, Yu HZ, Xu BQ, Peng LC(2023).Single-molecular insights into the breakpoint of cellulose nanofibers assembly during saccharification.Nat Commun., 14:1100-1100.