Squamosa promoter binding protein
(SBP) gene family is widely involved in plant growth and
development, signal transduction, and many physiological and biochemical
processes. However, the genes of the proso millet (
Panicum miliaceum)
SBP gene family have not been systematically studied. A total of 25
SBP genes were identified and systematically named in the proso millet genome, and
the
SBP genes were divided into 6 subfamilies through phylogenetic
analysis. The members of the same subfamily had similar gene structure and
conserved motifs. The collinearity analysis showed that 7 pairs of direct
homologous genes were formed with
Arabidopsis thaliana AtSBP and 31
pairs with
Oryza sativa OsSBP. The analysis of
cis-acting elements showed that the promoter region of
SBP gene in proso millet was rich in
elements related to stress, plant light response and plant hormone signal
response. The analysis of gene expression patterns showed that the
SBP gene in proso millet had obvious tissue specificity, and the
SBP gene
expression pattern was also specific in different varieties, and the expression
trend was different in different periods, suggesting that
SBP gene
should play an important role in the growth and development of proso millet.
The results laid a foundation for revealing the biological function of
SBP gene family in the growth and development of proso millet, and also provided a
reference for the research and application of other crops.