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Hosted by:Chinese Academy of Sciences
Sponsored by:Institute of Botany, Chinese Academy of Sciences, Botanical Society of China
Co-hosted by:Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences
Institute of Biotechnology and Germplasm Resources, Yunnan AgriculturalAcademy
Fujian Agriculture and Forestry University
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University
State Key Laboratory of Crops Biology, Shandong Agricultural University

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- Genome-wide Identification and Analysis of PLATZ Transcription Factor Gene Family in Foxtail Millet
- Hui ZHANG Xu-Kai LI
- Chinese Bulletin of Botany. 2023, 58(4): 548-559. doi:10.11983/CBB22147
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Abstract ( 321 )
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- Cloning and functional verification of the borneol dehydrogenase encoding gene AArBDH1 in Artemisia argyi
- Changjie Chen, Yuhuan Miao, Dandan Luo, Zixin Wang, Lujuan Guo, Tingting Zhao, Dahui Liu
- Chinese Bulletin of Botany. 2023, 58(4): 560-572. doi:10.11983/CBB22123
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Abstract ( 163 )
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- Modulatory effects of exogenous organic acids on the physiological response system of Helianthus tuberosus under aluminium stress
- Xuanwen Mao, Zhichao Wang, Xinyi Ruan, Jingfei Sun, Yating Zhang, Jinhao Lu, Tiantian Shao, Xian Wang, Jiamin Xiao, Li Xiao, Mengyao Ye, Yuhuan Wu, Peng Liu
- Chinese Bulletin of Botany. 2023, 58(4): 573-589. doi:10.11983/CBB23006
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Abstract ( 388 )
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- Regulation of Plasma Membrane Protein Dynamics and Its Research Methods
- Pengyun Luo, Hongping Qian, Changwen Xu, Yaning Cui
- Chinese Bulletin of Botany. 2023, 58(4): 590-601. doi:10.11983/CBB22102
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Abstract ( 141 )
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- Optimization and evaluation of Tn5 transposase fusion protein in CUT&Tag
- Shengyu Liu, Xiaobin Liu, Jiafu Zhu, Jing Su, Zhicheng Dong, Min Liu
- Chinese Bulletin of Botany. 2023, 58(4): 602-611. doi:10.11983/CBB22091
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- Research progress on molecular mechanism of starch accumulation in rice endosperm
- Ziwen Tang, Dongping Zhang
- Chinese Bulletin of Botany. 2023, 58(4): 612-621. doi:10.11983/CBB22071
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Abstract ( 331 )
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- Red and Far-red Light Regulation of Plant Growth, Development and Abiotic Stress Response
- Yanan Xu, Jiarong Yan, Xin Sun, Yufeng Liu, Zhouping Sun, Mingfang Qi, Tianlai Li, Feng Wang
- Chinese Bulletin of Botany. 2023, 58(4): 622-637. doi:10.11983/CBB22087
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- The Progress on Riboflavin Biosynthesis and Function in Plants
- Haitao Hu, Longbiao Guo
- Chinese Bulletin of Botany. 2023, 58(4): 638-655. doi:10.11983/CBB22109
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- Research Progress on Catalytic Characteristics of Pinoresinol-lariciresinol Reductase ( PLR ) in Plants
- Yanhong Yin, Wansheng Chen, Ying Xiao
- Chinese Bulletin of Botany. 2023, 58(4): 656-666. doi:10.11983/CBB22160
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The PLATZ transcription factor family is a class of plant-specific
zinc-dependent DNA-binding proteins that play an integral role in plant growth
and development and stress resistance. However, the PLATZ family genes
have not been systematically analyzed in foxtail millet (Setaria italica). In this study, 17 PLATZ genes were identified and systematically named in the foxtail millet genome.
The SiPLATZ genes were divided into five subfamilies by phylogenetic
analysis, and members of the same subfamily have similar gene structures and motifs.
Cis-acting element analysis demonstrated that the SiPLATZ genes may play
a role in seed endosperm expression and various stress-resistant responses. The
Ka/Ks ratio results indicate that duplicate genes are subject to purifying
selection.There were significant differences in the expression of SiPLATZ genes in different tissues and developmental stages, which were mainly divided
into two categories: high expression in roots,
leaves, and stems, and in spikes and seeds. That which reflects the complexity
of the physiological functions of SiPLATZ genes and their possible
involvement in regulating seed growth and multiple stress responses. In addition,
the co-expression network constructed in combination with WGCNA analysis
revealed that SiPLATZ6, SiPLATZ8, SiPLATZ9 and SiPLATZ11 may be candidates for genetic improvement of foxtail millet yield and
functional gene research. These results lay the foundation for deeper insight
into the biological functions of PLATZ transcription factors in foxtail millet
growth and development.
Borneol is one of the most important pharmacodynamic components in Artemisia
argyi, which has antibacterial, anti-inflammatory, analgesic and other
pharmacological activities. The synthesis and metabolism of borneol are
affected by many kinds of enzymes, and borneol dehydrogenase is one of the key
enzymes that can oxidize borneol to camphor. In this study, the contents of borneol and camphor
in 35 germplasm resources of A. argyi leaves were
determined by Gas chromatograph-mass spectrometer (GC-MS), based on the
full-length transcriptome results of A. argyi and homology comparison analysis, the first borneol dehydrogenase encoding gene AArBDH1 was cloned in A. argyi, the gene structure and the physical and chemical
properties of the encoded protein were analyzed, the function of the encoded
protein was identified. The results showed that the contents of borneol and camphor were quite various in different varieties, in some
germplasm, the content of camphor was even higher than borneol, indicating that
a large amount of borneol was oxidized to camphor, which seriously reduced the
content of borneol in A. argyi leaves. The AArBDH1 contained 2 exons and 1
intron, and the length of its open reading frame was 870 bp, which encoded 289
amino acids. AArBDH1 gene expression levels were
measured by qRT-PCR (Real-time quantitative
reverse transcription PCR) technology, the result showed
that AArBDH1 was differentially expressed in different tissues and
leaves at different development stages, and highly expressed in stem
and 30 days old leaves. The results of enzyme reactions using borneol as substrate and NAD+ as coenzyme showed that AArBDH1 could catalyze the dehydrogenation of
borneol to camphor. The study provides
a theoretical basis and gene resources for further analyzing the accumulating
regularity of borneol in A. argyi leaves and improving the content of borneol in A.
argyi leaves through molecular biology method.
Aluminum (Al) is one of the
common metal contaminants in acidic soils. To reveal the effects of exogenous
organic acids on the physiological characteristics and root DNA damage of Helianthus tuberosus under Al stress, we
aimed to improve the Al tolerance of Helianthus tuberosus and promote safe production in Al-contaminated areas.
The effects of exogenous organic acids on the physiological response and DNA
damage of Helianthus tuberosus at various periods (7, 14, and 21 d)
under Al stress were investigated by setting low, medium and high (0, 350 and
700 µmol·L-1) Al concentration treatments and applying 0, 30, 60 and
90 µmol·L-1 compound
organic acids, respectively. The results showed that Al stress inhibits root
elongation and root vitality, severely inhibited the photosynthetic and
antioxidant systems of Helianthus tuberosus, and the DNA damage in the
root system increased with the increase of Al concentration. In contrast, the
application of 60 µmol·L-1 compound
organic acid effectively alleviated Al stress. 60 μmol/L compound organic acid improved the
activity of the antioxidant system, maximum photochemical efficiency and
organic acid secretion in root tips, citric acid with secretion 2 times
(Xuzhou) and 0.75 times (Ziyang) than the control, reduced root tip aluminium
content and improved root vitality. Besides, Xuzhou Helianthus tuberosus and Ziyang Helianthus tuberosus oliver tail moment decreased by 51.53% and 35.10%, and compound organic acid reduced the DNA
trailing phenomenon and repaired DNA breaks to a greater extent. In conclusion,
high concentration of Al cause serious damage to Helianthus tuberosus and difficult to mitigate. 60 µmol·L-1 compound organic acid could enhance the Helianthus tuberosus physiological response under low aluminum stress, reduce DNA damage and improve
the stress resistance. The alleviation effect was better in Ziyang Helianthus
tuberosus. This study
reveals the regulatory role of exogenous organic acids on the physiological
response of chrysanthemum under Al stress, and provides a theoretical basis for
the Helianthus tuberosus and production
of other cash crops in the acid-aluminium areas of southern China.
Plasma membrane (PM) proteins are important
components of cell membranes and play
important roles in material transport, ion exchange, signal transduction, and
metabolism process. Their movements in the PM are in response to the
developmental cues and environmental stimuli. Studying the regulatory mechanism
of PM protein movement is crucial for a better understanding of the development
and the adaptation to environment of plants. In the recent years, the rapid
development of microscopic technologies enables us to move one step closer to
reveal the regulatory mechanism of PM protein dynamics. In this paper, we
systematically summarized PM protein dynamics and the factors affecting it. We
also provided an introduction to commonly-used microscopic imaging techniques
for PM protein dynamics research. This review will provide new insights into
further investigation of biological significance of PM proteins.
Tn5 is a bacterial transposon. The engineered Tn5 can efficiently
tagment DNA while adding the adapter sequences. Therefore, it has been widely
used in the preparation of high-throughput sequencing libraries. CUT&Tag
(Cleavage Under Target & Tagmentation) is an improved technology for
studying the interaction between protein and DNA, which has the advantages of
good repeatability, high signal-to-noise ratio, and easy operation. This
technology uses Protein A (pA) or Protein G (pG) and Tn5 to form a fusion
protein, which can locate specific antibodies (the antibody is used to identify
the target protein), and break the DNA near the target site while introducing
the sequencing adapters. Then DNA is extracted, followed by a PCR amplify to
obtain the sequencing library. However, different types of antibodies have
different affinities with pA and pG, thus limiting the application of CUT&Tag
for some antibodies. In order to overcome this limitation, the expression
vector of pG-Tn5 was constructed by recombination, and pG-Tn5 recombinant
protein was obtained by prokaryotic expression and affinity purification. We
used RNA polymerase II (Pol II) specific antibody (Pol II Ser5P, Mouse IgG1 and
Rabbit IgG) to comparing the efficiency of pA-Tn5 and pG-Tn5 in library preparation
of CUT& Tag in Arabidopsis. The results showed that the IgG1 antibody had
higher affinity with pG-Tn5, and the quality of the constructed library was
better when pG-Tn5 was used. While the Rabbit IgG antibody has comparable
affinities to the two enzymes. And a lower starting amount of plant material can
be applicable in CUT&Tag. This study provides a reference for the selection
of Tn5 fusion proteins against different antibodies in future CUT&Tag
experiments.
Starch
is the major storage material of rice (Oryza
sativa) endosperm, and its accumulation process affects the subsequent
growth and development of plants. At the same time, as one of the main
nutrients absorbed by human beings from rice, the synthesis and accumulation process
of starch in rice has attracted more and more attention. This review mainly discusses
the latest progress of endogenous factors affecting starch synthesis and accumulation,
such as sucrose unloading from phloem, key enzymes of starch synthesis and
hormones, and points out the unsettled questions in the field of endosperm
starch accumulation, so as to provide some reference ideas for further research
in the future.
As one of the important environmental factors, light
not only provides energy for plant growth and development through
photosynthesis, but also affects plant response to the external environment as
a signal. Here, we summarize the regulation of red light and far-red light on
plant growth and development, and abiotic stress response. This review focuses
on the mechanism of phytochromes and light signaling factors regulation of seed
germination, hypocotyl growth, bud development and flowering in plants through
integrating with endogenous signal transduction, such as hormones. In addition,
the regulation mechanism of red light and far-red light on plant response to
salt, drought and temperature stress was elucidated. It is expected that on the
basis of exploring the mechanism of plants' perception and response to light
environment, we can accurately supplement light for crops to improve crop
yield, quality and stress resistance through using LED spectrum technology,
meanwhile promoting the national goal of "carbon peak" and
"carbon neutral" to reduce energy consumption and environmental
pollution.
Riboflavin is the precursor of FMN and FAD that serve as indispensable
cofactor to maintain normal metabolism, which plays pivotal roles in
mitochondrial electron transport chain, citric acid cycle, β-oxidation of fatty
acids, branched-chain amino acid catabolism, redox homeostasis, chromatin
remodeling, DNA repair, apoptosis and secondary metabolite biosynthesis.
Riboflavin defect will cause metabolic disorders and a series of defective
phenotypes, and death in the severe cases. Among the living organisms,
microorganisms and plants can de novo synthesize riboflavin, but humans and
animals only obtain it from food. At present, the regulation of riboflavin
biosynthesis in microorganisms has been clearly studied, but the mechanism of
riboflavin transport and metabolism in plants is still not clear. Therefore,
isolating riboflavin deficient mutants is of great significance for analyzing
the molecular mechanisms of riboflavin biosynthesis, transport, and metabolism
in plants and the effect of riboflavin on plant growth and development. In this
paper, we firstly review riboflavin biosynthetic pathway and key enzymes, and
then the processes of riboflavin involved in plant growth and development in
detail, and finally give prospects for plant riboflavin research.
Pinoresinol–lariciresinol reductases (PLR) are key enzymes involved in the lignan biosynthesis in plants, which convert pinoresinol to lariciresinol and then to secoisolariciresinol. PLR are NADPH-dependent reductases with substrate stereoselectivity. The catalytic products of PLR are source of different types of 8-8′ lignans, and the substrate selectivity directly determines the skeleton types of lignans, such as furano, dibenzylbutane, dibenzylbutyrolactone and aryltetrahydronaphthalene lignans. Therefore, the catalytic and expression characteristics of PLR play an important role in the composition and biodiversity of lignans in plants. This paper reviewed the research progress in the important role of PLR in lignans biosynthesis, as well as its enantioselectivity and catalytic mechanism, thus to lay the foundation for further study on the biological function and catalytic mechanism of PLR gene, and point out the direction for the precise biosynthesis of different types of lignans enantiomers through synthetic biology or plant secondary metabolic engineering.