Establishment and Application of a High-throughput Screening Method for Salicylic Acid Metabolic Mutants in Rice

doi:10.11983/CBB24148

Abstract

Abstract: Salicylic acid (SA) plays essential roles in plant immunity. Quantitative analysis of SA in plants is essential for studying SA metabolism and biological function. Although high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC/MS) are widely used for SA determination, but it is difficult to achieve high throughput determination. However, SA synthesis pathway in rice has not yet to be fully elucidated, efficient screening method of SA-related mutants is of great significance for the analysis of the metabolic pathway. In this paper, we improved the analytical method for quantifying SA based on biosensor Acinetobacter sp.ADPWH_lux, and established a rapid high-throughput method for SA quantification in rice with simplifying the sample collection and extraction process. It avoids the time-consuming steps such as sample weighing, tissue grinding and centrifugation, and the entire process is convenient and efficient. We demonstrated the method's feasibility using the published rice SA metabolism mutants. Then, we screened some rice mutants from Co-60 mutant library with high or low SA metabolism using this method and confirmed the source SA level of the mutants by HPLC. Our results showed that this method could be used to screen SA related metabolic mutants, and has important application potential for studying the role of SA metabolism and biological could function in rice and other crops.

Can Ye, Linbo Yao, Ying Jin, Rong Gao, Qi Tan, Xuying Li, Yanjun Zhang, Xifeng Chen, Bojun Ma, Wei Zhang, Kewei Zhang. Establishment and Application of a High-throughput Screening Method for Salicylic Acid Metabolic Mutants in Rice[J]. Chinese Bulletin of Botany, DOI: 10.11983/CBB24148.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24148

References

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