Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak

doi:10.11983/CBB23071

Abstract

Abstract: Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is one of the most important diseases of rice, which is now highly prevalent in rice-growing regions of China, especially in Southern China (including Jiangsu, Zhejiang, Fujian, and Guangdong). Planting resistant varieties is considered as the best approach to control BLS. However, no resistant cultivars are available owing to limited genetic resources for BLS resistance. Two highly BLS-resistant materials (M1 and D1) have been discovered in our research by screening germplasm against BLS via syringe inoculation. Multi-strain inoculation showed that M1 had the characteristics of race-nonspecific broad-spectrum resistance (RNS BSR). The genetic population analysis showed that the cultivated rice M1 harbored a single dominant new gene Xo-3 of resistance to BLS. Through BSA-seq and association analysis, Xo-3 was initially mapped in a candidate region on chromosome 2. The mining of germplasm resources of BLS-resistant and the analysis of the genetic basis of its resistance will help our understanding of the interaction mechanism between rice and Xoc, so as to cultivate new varieties of BLS-resistant rice and development scientific strategies in controlling BLS.

Key words: bacterial leaf streak, germplasm resources, resistance gene, rice, Xanthomonas oryzae

Yanli Fang, Chuanyu Tian, Ruyi Su, Yapei Liu, Chunlian Wang, Xifeng Chen, Wei Guo, Zhiyuan Ji. Mining and Preliminary Mapping of Rice Resistance Genes Against Bacterial Leaf Streak[J]. Chinese Bulletin of Botany, 2024, 59(1): 1-9.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB23071

https://www.chinbullbotany.com/EN/Y2024/V59/I1/1

Figures/Tables 6

Figure 1 Diagram for the identification of rice germplasms that confer resistance to bacterial leaf streak (BLS) (A) Occurrence of bacterial leaf streak in paddy (bar=1 cm); (B) Syringe inoculation; (C) Rice materials for resistance identification

Figure 2 Identification of rice germplasms that confer resistance to bacterial leaf streak (BLS) with needleless syringes (A) Phenotype of representative rice materials; (B) Lesion length of representative rice materials (different lowercase letters above horizontal columns indicate significant statistical differences at P<0.01); (C) Distribution of rice materials in BLS disease reactions (R: High resistance; MR: Middle resistance; S: Susceptibility; HS: High susceptibility)

Figure 3 Identification of M1 and D1 that confer resistance to bacterial blight (BB) (A) Disease response of M1 and D1 inoculated with Xoo (PXO99A) (bar=1 cm); (B) Sequence of Xa5/xa5 locus in M1 and D1 (the red dashed box represents the mutation sites)

Figure 4 Bacterial leaf streak (BLS) resistance spectrum assay of M1 (A) Phenotype of hypervirulent Xoc strains in Nip and M1 (bar=0.5 cm); (B) Lesion length of hypervirulent Xoc strains in Nip and M1 (** indicate extremely significant differences (P<0.01))

Figure 5 Genetic analysis of M1 that confers resistance to bacterial leaf streak (BLS) (A) Resistance identification of F2 plants derived from the cross between Nipponbare and M1 (bar=0.5 cm); (B) Statistical analysis of resistant and susceptible F2 plants. R, MR, S, and HS are the same as shown in Figure 2.

Figure 6 Mapping of bacterial leaf streak (BLS) resistance gene Xo-3 in M1 using BSA-Seq method The red dashed box represents the candidate gene region.

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