Response Mechanism of Rice Mutant pe-1 to Low Light Stress

doi:10.11983/CBB24039

Abstract

Abstract: This study utilized the γ-ray-induced early-maturation, fresh-green mutant line pe-1 from indica rice as an experimental material. At the trilobal stage and the tillering stage, we observed differences in morphological characteristics between pe-1 and wild type. In addition, we measured the activity of antioxidant-related enzymes and their regulatory genes expression, chlorophyll content and chloroplast synthesis and degradation-related gene expression, and photomorphogenesis-related gene expression to detect the differences in the low light response between the pe-1 and wild type. The results showed that pe-1 exhibited less leaf yellowing, taller stature, and larger leaf area compared to wild type post-stress. The changes in chlorophyll content differed between leaves at the trilobal stage and the tillering stage. Additionally, pe-1 resulted in increased chlorophyll content and elevated levels of the stress-responsive enzymes catalase and peroxidase, as well as increased expression of related genes. This indicates enhanced reactive oxygen species sca- venging and stronger adaptability to adverse conditions under low light conditions. Moreover, pe-1 exhibited increased expression levels of genes associated with photomorphogenesis, indicating superior light perception ability under low light intensities. In summary, the pe-1 mutant shows immense potential for survival under low light stress, contributing to the breeding rice with low light tolerance.

Key words: rice, pe-1, low light stress, photomorphogenesis, chlorophyll, chloroplast synthesis and degradation

Jiahui Huang, Huimin Yang, Xinyu Chen, Chaoyu Zhu, Yanan Jiang, Chengxiang Hu, Jinjin Lian, Tao Lu, Mei Lu, Weilin Zhang, Yuchun Rao. Response Mechanism of Rice Mutant pe-1 to Low Light Stress[J]. Chinese Bulletin of Botany, 2024, 59(4): 574-584.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24039

https://www.chinbullbotany.com/EN/Y2024/V59/I4/574

Figures/Tables 10

References 25

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Component	Volume (μL)
500 ng∙µL^-1 cDNA	1
10 µmol∙L^-1forward primer	1
10 µmol∙L^-1reverse primer	1
ddH₂O	2
2× SYBR Mix	5

Component	Volume (μL)
500 ng∙µL^-1 cDNA	1
10 µmol∙L^-1forward primer	1
10 µmol∙L^-1reverse primer	1
ddH₂O	2
2× SYBR Mix	5

Reaction temperature (°C)	Function	Reaction time	Cycle number
95	Predegeneration	5 min	1
95	Denaturation	$ \left.\begin{array}{l} 5 \mathrm{~s} \\ 20 \mathrm{~s} \\ 20 \mathrm{~s} \end{array}\right\}$	39
55	Annealing
72	Extension
72	Final extension	10 min	1
15	Hold	Indefinite	1

Reaction temperature (°C)	Function	Reaction time	Cycle number
95	Predegeneration	5 min	1
95	Denaturation	$ \left.\begin{array}{l} 5 \mathrm{~s} \\ 20 \mathrm{~s} \\ 20 \mathrm{~s} \end{array}\right\}$	39
55	Annealing
72	Extension
72	Final extension	10 min	1
15	Hold	Indefinite	1

Traits	Primer name	Forward primer (5′-3′)	Reverse primer (5′-3′)
	OsActin	TGGCATCTCAGCACATTCC	TGCACAATGGATGGGTCAGA
Chloroplast synthesis	LOC_Os03g20700	GGGAACTTGGCGTTTCATTA	TCAAATGTCTTGCGTTGCTC
and degradation	LOC_Os03g59640	AAAGAATGGCTCGAAAAGCA	GCCACCACATGGTAGCTTCT
	LOC_Os08g06630	TGGATCAACAAGCATTTCCA	GACAACCCGAATTGTCTGCT
	LOC_Os03g19510	GACCCGTGGAGGATGATATG	GCTGTATCGCTTCCCTTTTG
	LOC_Os03g27770	ACCGACGTCGCCTATGTTTA	TATAGCCACCCCTCCAGTTG
	LOC_Os05g28200	AAGCAGAATGGATGGATTGG	GAGTGACTGAAGCCCCAGAG
	LOC_Os03g45194	TGGTGCAACGAAGAGAAGTG	ACGAGATAATCCGCAACCAC
	LOC_Os06g07210	AAGCAAATCTGCTGGAGGAA	AAAAATATCCGCATGCCAAG
Enzyme activity	LOC_Os02g02400	GGCGTCAACACCTACACCTT	GTCGAACCTCTCCTCCTCCT
	LOC_Os06g51150	GTGAATGCACCAAAATGTGC	CCAGCCTGTTGGAAATTGTT
	LOC_Os03g03910	CCACCACAACAACCACTACG	AGTGCGTCGATCCATCTCTT
	LOC_Os03g57220	CCTCTGGAGCTGAGGATGAG	CCCTGGCTGTTGCATACTCT
	LOC_Os04g53210	GGCTGAATTGGCTGGTTTTA	GTCCGTCCAAGACAGAGAGC
	LOC_Os07g05820	ATTGCGCTTACGGTGGATAC	GATCACTCCCTTCACCAGGA
	LOC_Os01g17260	GGAAGACACCAGCTGAGAGG	CAAGAGACCCAGCCAATGTT
Photomorphogenesis	LOC_Os05g11510	GAGCCACGCGTACTTCTTCT	GGTGGTCGCTCTTGGATG
	LOC_Os02g10860	GCCAAAAAGCTCTCATGGTC	GGAAGGAGAAGGGATCGAAG
	LOC_Os03g51030	AAGAGCTTGCCTTGGAATGA	GGGACAGTTGCTGTTTCCAT
	LOC_Os03g19590	CTCGCCTACTCCGAGAACAC	AGGATGGCGTAGAAGGGTTT
	LOC_Os02g36380	CGTCTACGTCAGAGGCATCA	CTTCCCAGCCTGTTGTCAAT
	LOC_Os04g37920	GATGAAGCAGCTTGTGTGGA	AACAAGGGGGTAACCAGTCC
	LOC_Os06g40080	TCAAGGAACAGGGTCACACA	TGCCCTCCCATTTGTAGAAC