Mapping of QTL for Heat Tolerance at Seedling Stage in Rice Based on a High-Density Bin Map

doi:10.11983/CBB24047

Abstract

Abstract: Identification of new loci and genes related to heat tolerance is very important for the genetic mechanism research and breeding of new heat-tolerant rice varieties. A recombinant inbred lines (RILs) was developed by crossing the japonica rice TD70 and the indica rice Kasalath. The high-density genetic linkage map with recombination Bin markers was constructed based on the re-sequencing. Based on the genotype and phenotype data of the RILs, QTL mapping of the high temperature seedling survival rate (HTSR) was performed by ICIM method of the QTL Ici Mapping software. 26 QTLs related to HTSR of rice were detected, distributed on 12 chromosomes except 3. The LOD value of single QTL ranged from 2.59–16.15, respectively. Among of them, four QTLs were detected with LOD values greater than 10. Seven QTLs were found to locate in the same interval or adjacent to known heat tolerance QTLs. Major locus qHTSR5.1 located in the 20.41-20.48 Mb of Chr. 5 with LOD value 12.07, which explained 4.86% of the total phenotypic variation for HTSR. According to the annotation and sequences analysis of genes located in the region of four major QTLs, we found that twenty-seven annotated genes with non-synonymous mutations in the coding regions between TD70 and Kasalath. The HTSR of RILs had significant difference between the RILs with different haplotype of five genes, indicating that they might be the candidate genes for HTSR.

Key words: heat tolerance, high-density bin map, QTL mapping, rice, seedling stage

Ling Zhao, Ju Guan, Wenhua Liang, Yong Zhang, Kai Lu, Chunfang Zhao, Yusheng Li, Yadong Zhang. Mapping of QTL for Heat Tolerance at Seedling Stage in Rice Based on a High-Density Bin Map[J]. Chinese Bulletin of Botany, 2025, 60(3): 1-0.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB24047

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References

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