Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (6): 882-892.DOI: 10.11983/CBB23100

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Mapping of QTL for Cell Wall Related Components in Rice Stem and Analysis of Candidate Genes

Qiwei Jia1,, Qianqian Zhong1,, Yujia Gu1, Tianqi Lu1, Wei Li1, Shuai Yang1, Chaoyu Zhu1, Chengxiang Hu1, Sanfeng Li2, Yuexing Wang2,*(), Yuchun Rao1,*()   

  1. 1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2023-07-28 Accepted:2023-11-02 Online:2023-11-01 Published:2023-11-27
  • Contact: * E-mail:;
  • About author: These authors contributed equally to this paper

Abstract: Lodging of rice is one of the main factors reducing rice yield. The mechanical strength of stem affects lodging resistance of rice and is closely related to the content of stem cell wall related components. Improving lodging resistance of rice by regulating the related components in the cell wall of stem is an effective way to improve the yield and quality of rice. In this study, indica rice variety Huazhan (Oryza sativa subsp. indica cv. ‘HZ’) and japonica rice variety Nekken2 (O. sativa subsp. japonica cv. ‘Nekken2’) were crossed to obtain F1 generation. A total of 120 RILs (recombinant inbred lines) population were obtained by successive multigeneration self-crossing and the genetic linkage map was constructed. Based on the constructed high-density genetic map, the QTLs related to the content of cellulose, hemicellulose, and lignin in the cell wall of rice stem were located and analyzed. The results showed that 4 QTLs related to cellulose, 12 QTLs related to hemicellulose, and 8 QTLs related to lignin. At the same time, candidate genes analyses were conducted on the detected QTLs’ intervals, and a total of 13 candidate genes were screened. The expression levels of candidate genes were detected by qRT-PCR. Except for LOC_Os02g58590 and LOC_Os12g41720, the other candidate genes showed significant differences between parents. The results laid an important foundation for exploring genes that regulate the mechanical strength of rice stems, further screening and breeding rice varieties with strong lodging resistance.

Key words: Oryza sativa, mechanical strength of stem, QTL mapping, candidate genes, lodging-resistant