Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (3): 275-283.DOI: 10.11983/CBB21039
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Chenyang Pan, Yue Zhang, Han Lin, Qianyu Chen, Kairu Yang, Jiaji Jiang, Mengjia Li, Tao Lu, Kexin Wang, Mei Lu, Sheng Wang, Hanfei Ye, Yuchun Rao*(), Haitao Hu*()
Received:
2021-02-22
Accepted:
2021-03-26
Online:
2021-05-01
Published:
2021-04-30
Contact:
Yuchun Rao,Haitao Hu
Chenyang Pan, Yue Zhang, Han Lin, Qianyu Chen, Kairu Yang, Jiaji Jiang, Mengjia Li, Tao Lu, Kexin Wang, Mei Lu, Sheng Wang, Hanfei Ye, Yuchun Rao, Haitao Hu. QTL Mapping and Candidate Gene Analysis on Rice Leaf Water Potential[J]. Chinese Bulletin of Botany, 2021, 56(3): 275-283.
Primer name | Sequence (5′-3′) | Tm (°C) | Length (bp) |
---|---|---|---|
LOC_Os02g56630-F-qrt | GATGCTACGGAGACTGCACA | 60.02 | 182 |
LOC_Os02g56630-R-qrt | GAGCCAGAAGCACATGAACA | 59.99 | |
LOC_Os02g57720-F-qrt | CTGACGTCGTGGTCGTTCTA | 59.90 | 101 |
LOC_Os02g57720-R-qrt | GACTTGTTCACCCCCATCAC | 60.22 | |
LOC_Os04g43730-F-qrt | ACCAAAGTGGGTGTTTCTCG | 60.01 | 153 |
LOC_Os04g43730-R-qrt | TCGAGATCTGGCTTGTGTTG | 59.98 | |
LOC_Os04g44060-F-qrt | CGGTGTTCATGGTTCACTTG | 60.00 | 182 |
LOC_Os04g44060-R-qrt | CCTCAGGACGTACTGGTGGT | 60.03 | |
LOC_Os04g46490-F-qrt | GCCTCGTCCTCCACTACATC | 59.69 | 123 |
LOC_Os04g46490-R-qrt | CCGTGTACACCACCATGAAC | 59.73 | |
LOC_Os02g57580-F-qrt | ACCTCTTCAGATGGGGTGTG | 59.96 | 101 |
LOC_Os02g57580-R-qrt | CCAGTCAGTTTTGCAGACCA | 59.87 | |
LOC_Os04g48230-F-qrt | GGGCACTACAAGTCCGTGAT | 60.00 | 199 |
LOC_Os04g48230-R-qrt | CTTGGTAGCTTCCGATGAGC | 59.98 | |
LOC_Os04g44570-F-qrt | CGCCACCACTGGGTTTACT | 60.96 | 123 |
LOC_Os04g44570-R-qrt | CACGGGAAGCCGAGTATCT | 60.23 |
Table 1 The primer sequences of real-time quantitative PCR
Primer name | Sequence (5′-3′) | Tm (°C) | Length (bp) |
---|---|---|---|
LOC_Os02g56630-F-qrt | GATGCTACGGAGACTGCACA | 60.02 | 182 |
LOC_Os02g56630-R-qrt | GAGCCAGAAGCACATGAACA | 59.99 | |
LOC_Os02g57720-F-qrt | CTGACGTCGTGGTCGTTCTA | 59.90 | 101 |
LOC_Os02g57720-R-qrt | GACTTGTTCACCCCCATCAC | 60.22 | |
LOC_Os04g43730-F-qrt | ACCAAAGTGGGTGTTTCTCG | 60.01 | 153 |
LOC_Os04g43730-R-qrt | TCGAGATCTGGCTTGTGTTG | 59.98 | |
LOC_Os04g44060-F-qrt | CGGTGTTCATGGTTCACTTG | 60.00 | 182 |
LOC_Os04g44060-R-qrt | CCTCAGGACGTACTGGTGGT | 60.03 | |
LOC_Os04g46490-F-qrt | GCCTCGTCCTCCACTACATC | 59.69 | 123 |
LOC_Os04g46490-R-qrt | CCGTGTACACCACCATGAAC | 59.73 | |
LOC_Os02g57580-F-qrt | ACCTCTTCAGATGGGGTGTG | 59.96 | 101 |
LOC_Os02g57580-R-qrt | CCAGTCAGTTTTGCAGACCA | 59.87 | |
LOC_Os04g48230-F-qrt | GGGCACTACAAGTCCGTGAT | 60.00 | 199 |
LOC_Os04g48230-R-qrt | CTTGGTAGCTTCCGATGAGC | 59.98 | |
LOC_Os04g44570-F-qrt | CGCCACCACTGGGTTTACT | 60.96 | 123 |
LOC_Os04g44570-R-qrt | CACGGGAAGCCGAGTATCT | 60.23 |
QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Likelihood of odd (LOD) |
---|---|---|---|---|
qLpw2-8 | 2 | 34636269-35675126 | 148.48-152.93 | 3.27 |
qLpw3-8 | 3 | 30389734-30481217 | 130.27-130.66 | 2.93 |
qLpw4-8 | 4 | 24066261-30847136 | 103.17-132.23 | 5.15 |
qLpw11-8 | 11 | 2051418-2119803 | 8.79-9.09 | 3.06 |
qLpw12-8 | 12 | 25396321-25626163 | 108.87-109.85 | 3.34 |
Table 2 QTL analysis of leaf water potential in rice at tillering stage
QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Likelihood of odd (LOD) |
---|---|---|---|---|
qLpw2-8 | 2 | 34636269-35675126 | 148.48-152.93 | 3.27 |
qLpw3-8 | 3 | 30389734-30481217 | 130.27-130.66 | 2.93 |
qLpw4-8 | 4 | 24066261-30847136 | 103.17-132.23 | 5.15 |
qLpw11-8 | 11 | 2051418-2119803 | 8.79-9.09 | 3.06 |
qLpw12-8 | 12 | 25396321-25626163 | 108.87-109.85 | 3.34 |
Chromosome | Gene | Function | Regulation object | Reference |
---|---|---|---|---|
2 | LOC_Os02g56630 | Play an important regulatory role in the response of plants to abiotic stress | OsWAK24-OsWAK receptor-like protein kinase | |
4 | LOC_Os04g43730 | Play an important regulatory role in the response of plants to abiotic stress | OsWAK51-OsWAK receptor-like protein kinase | |
2 | LOC_Os02g57580 | Maybe involved in anthocyanin vacuole storage, participate in osmotic adjustment | Anthocyanin permease | Aza-González et al., 2013 |
2 | LOC_Os02g57720 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g46490 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g44570 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g44060 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g48230 | Maybe involved in the perception, conduc- tion of plant dehydration and regulation of antidehydration substance synthesis | Dehydration response related protein | Shinozaki and Yamaguchi-Shinozaki, 1997 |
Table 3 The function annotation of candidate genes
Chromosome | Gene | Function | Regulation object | Reference |
---|---|---|---|---|
2 | LOC_Os02g56630 | Play an important regulatory role in the response of plants to abiotic stress | OsWAK24-OsWAK receptor-like protein kinase | |
4 | LOC_Os04g43730 | Play an important regulatory role in the response of plants to abiotic stress | OsWAK51-OsWAK receptor-like protein kinase | |
2 | LOC_Os02g57580 | Maybe involved in anthocyanin vacuole storage, participate in osmotic adjustment | Anthocyanin permease | Aza-González et al., 2013 |
2 | LOC_Os02g57720 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g46490 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g44570 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g44060 | Mediate the transport of water across the membrane, regulate water | Aquaporin | |
4 | LOC_Os04g48230 | Maybe involved in the perception, conduc- tion of plant dehydration and regulation of antidehydration substance synthesis | Dehydration response related protein | Shinozaki and Yamaguchi-Shinozaki, 1997 |
Figure 4 Differences in the expression level of candidate genes involved in leaf water potential in rice ** indicate significant differences in genes expression level between HZ and Nekken2 at 0.01 level.
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