Genetic Analysis of Four Stigma Traits with Genic Male Sterile Line in Rice (Oryza sativa)

doi:10.11983/CBB20200

Abstract

Abstract: To provide genetic information suitable for improving stigma-related traits of genic male sterile lines in rice. We investigated four stigma-related traits of 7001S (japonica genic male sterile line, P₁), Z913S (indica genic male sterile line, P₂), F₁ (P₁×P₂), F₂ and F_2:3. Coefficients of linear correlation among four stigma-related traits were calculated, and genetic patterns were analyzed using mixed major-gene plus polygene inheritance models. We found an extremely significant linear positive correlation among four stigma traits, and the correlation coefficients were between 0.274 and 0.897. Genetic analysis revealed that, style length (SYL) and percentage of exserted stigma (PES) were controlled by two major genes with additive-dominant effects and one major gene with additive-equal-negative-dominant effects plus polygenes in F_2:3, respectively. Stigma length (STL), SYL (except F_2:3), the sum of stigma and style length (TSSL), and PES (except F_2:3) were controlled by two major genes plus polygenes in F₂and F_2:3. The two major genes expressed additive-dominant-epistatic effects but expressed additive-dominant effects of SYL in the F_2:3 populations. Four stigma traits were mainly governed by major genes in two generations.

Key words: rice, genic male sterile line, stigma trait, major gene plus polygene model, genetic analysis

Jianhua Jiang, Xiaojing Dang, Wenhao Yao, Mengzhu Hu, Yuting Wang, Changmin Hu, Ying Zhang, Dezheng Wang. Genetic Analysis of Four Stigma Traits with Genic Male Sterile Line in Rice (Oryza sativa)[J]. Chinese Bulletin of Botany, 2021, 56(3): 284-295.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB20200

https://www.chinbullbotany.com/EN/Y2021/V56/I3/284

Figures/Tables 9

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Traits	Stigma length	Style length	The sum of stigma and style length	Percentage of exserted stigma
Stigma length	-	0.432**	0.897**	0.298**
Style length	0.526**	-	0.847**	0.320**
The sum of stigma and style length	0.792**	0.893**	-	0.365**
Percentage of exserted stigma	0.386**	0.274**	0.383**	-

Traits	Stigma length	Style length	The sum of stigma and style length	Percentage of exserted stigma
Stigma length	-	0.432**	0.897**	0.298**
Style length	0.526**	-	0.847**	0.320**
The sum of stigma and style length	0.792**	0.893**	-	0.365**
Percentage of exserted stigma	0.386**	0.274**	0.383**	-

Trait	Environment	Parent		F₁	F₂/F_2:3population
Trait	Environment	7001S	Z913S	F₁	Range	Means±standar-d deviation	Coefficient of variation (%)	Heritability (%)	Skewness	Kurtosis
Stigma length	E1	0.915	1.571**	1.237	0.782-1.666	1.145±0.143	12.48	82.85	0.54	0.88
Stigma length	E2	0.915	1.511**	1.204	0.690-1.560	1.122±0.148	13.24	82.37	0.11	0.23
Style length	E1	0.721	0.931**	0.852	0.468-1.160	0.782±0.119	15.20	84.38	0.69	0.36
Style length	E2	0.600	0.924**	0.790	0.394-1.283	0.786±0.201	15.62	82.66	0.11	-0.86
The sum of stigma and style length	E1	1.636	2.502**	2.089	1.406-2.617	1.927±0.229	11.88	91.73	0.63	0.30
The sum of stigma and style length	E2	1.521	2.434**	1.994	1.218-2.618	1.907±0.297	15.58	92.54	0.14	-0.50
Percentage of exserted stigma	E1	14.82	51.00**	37.88	3.24-78.52	30.64±15.22	49.68	86.80	0.76	0.47
Percentage of exserted stigma	E2	22.83	50.37**	38.21	2.33-80.79	40.44±18.01	44.53	90.63	0.10	-0.76

Trait	Environment	Parent		F₁	F₂/F_2:3population
Trait	Environment	7001S	Z913S	F₁	Range	Means±standar-d deviation	Coefficient of variation (%)	Heritability (%)	Skewness	Kurtosis
Stigma length	E1	0.915	1.571**	1.237	0.782-1.666	1.145±0.143	12.48	82.85	0.54	0.88
Stigma length	E2	0.915	1.511**	1.204	0.690-1.560	1.122±0.148	13.24	82.37	0.11	0.23
Style length	E1	0.721	0.931**	0.852	0.468-1.160	0.782±0.119	15.20	84.38	0.69	0.36
Style length	E2	0.600	0.924**	0.790	0.394-1.283	0.786±0.201	15.62	82.66	0.11	-0.86
The sum of stigma and style length	E1	1.636	2.502**	2.089	1.406-2.617	1.927±0.229	11.88	91.73	0.63	0.30
The sum of stigma and style length	E2	1.521	2.434**	1.994	1.218-2.618	1.907±0.297	15.58	92.54	0.14	-0.50
Percentage of exserted stigma	E1	14.82	51.00**	37.88	3.24-78.52	30.64±15.22	49.68	86.80	0.76	0.47
Percentage of exserted stigma	E2	22.83	50.37**	38.21	2.33-80.79	40.44±18.01	44.53	90.63	0.10	-0.76

Traits	Source of variation	Degrees of freedom	Sum of squares	Mean square	F value	F_0.05	F_0.01
Stigma length	Genotypes	2	6.34	3.17	876.00**	3.10	4.85
	Environments	1	0.02	0.02	6.83*	3.95	6.93
	Genotype × Environment	2	0.01	0.01	1.51	3.10	4.85
Style length	Genotypes	2	1.10	0.55	86.84**	3.10	4.85
	Environments	1	0.08	0.08	12.93**	3.95	6.93
	Genotype × Environment	2	0.04	0.02	3.19	3.10	4.85
The sum of stigma and style length	Genotypes	2	12.67	6.33	1174.95**	3.10	4.85
	Environments	1	0.20	0.20	36.45**	3.95	6.93
	Genotype × Environment	2	0.01	0.00	0.86	3.10	4.85
Percentage of exserted stigma	Genotypes	2	16677.93	8338.96	278.09**	3.10	4.85
	Environments	1	136.20	136.20	4.54*	3.95	6.93
	Genotype × Environment	2	327.42	163.71	5.46**	3.10	4.85