Chinese Bulletin of Botany ›› 2024, Vol. 59 ›› Issue (1): 10-21.DOI: 10.11983/CBB22226

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

OsCKX9 is Involved in Regulating the Rice Lamina Joint Development and Leaf Angle

Bao Zhu, Jiangzhe Zhao, Kewei Zhang, Peng Huang*()   

  1. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
  • Received:2022-09-28 Accepted:2022-12-13 Online:2024-01-01 Published:2023-01-16
  • Contact: *E-mail:

Abstract: The erection of rice leaf is one of the important agronomic traits that determine plant architecture, photosynthetic efficiency, and crop yield. Cytokinin is one of the most important plant hormones that regulate crop morphology, stress resistance and yield, but its role in the lamina joint development and leaf angle is still need to be further studied. Here, we report that rice CYTOKININ OXIDASE/DEHYDROGENASE9 (OsCKX9) controls lamina joint development and positively regulates leaf angle. Histological sections indicated that the leaf inclination changes in the WT and osckx9 resulted from the asymmetric proliferation of cells and vascular bundles in lamina joint. qRT-PCR showed that OsCKX9 was highly expressed in lamina joint. Quantification of cytokinin content in osckx9 mutant lamina joint showed that there were a mass of cytokinin accumulated. Moreover, the osckx9 showed insensitive to eBL. Therefore, our results revealed that OsCKX9 played a positive role in regulating leaf erectness, which provides genetic resources for analyzing the genetic basis of leaf angle and molecular-breeding of the ideal plant architecture rice.

Key words: rice, leaf angle, cytokinin, OsCKX9