Chinese Bulletin of Botany ›› 2023, Vol. 58 ›› Issue (3): 394-403.DOI: 10.11983/CBB22259

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

QTL Mapping and Expression Analysis on Candidate Genes Related to Chlorophyll Content in Rice

Jiayi Jin1, Yiting Luo1, Huimin Yang1, Tao Lu1, Hanfei Ye1, Jiyi Xie1, Kexin Wang1, Qianyu Chen1, Yuan Fang1, Yuexing Wang2(), Yuchun Rao1()   

  1. 1College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Abstract: Rice (Oryza sativa) is one of a major food crops in China. Increasing chlorophyll content in rice leaves and thus promoting its photosynthetic efficiency is one of the most important ways to obtain high and stable yields. In this study, 120 recombinant inbred lines (RILs) were constructed from the paternal indica rice HZ and the maternal japonica rice Nekken2 and they were used as experimental materials. We measured the flag leaf chlorophyll content (SPAD) of the parents and their progeny at the tillering and maturing stages. Meanwhile, the quantitative trait locus (QTL) mapping was carried out based on the high-density genetic map. Twenty QTLs related to chlorophyll content (7 at tillering stage and 13 at maturity stage) were identified, with the highest LOD score of 4.77. The expression of candidate genes associated with chlorophyll content within these QTL regions was analyzed by qRT-PCR, which showed that LOC_Os06g11780, LOC_Os06g12360, LOC_Os06g39716, LOC_Os08g42610, LOC_Os02g18500, LOC_Os03g21240, LOC_Os03g21400, LOC_Os03g21780, LOC_Os03g30950, and LOC_Os03g40550 were significantly different between the parents. Combining the gene expression and parental chlorophyll phenotype data, we think that the high expression of LOC_ Os06g11780, LOC_Os06g12360 and LOC_Os08g42610 might greatly enhance the chlorophyll content of rice, which in turn effectively promoted the photosynthetic productivity of the plants. The results provide favorable genetic resources for screening and breeding new rice varieties for efficient light energy utilization, and lay an important foundation for revealing the dynamic change pattern and the molecular regulation mechanism of chlorophyll content in rice.

Key words: candidate genes, chlorophyll content, genetic map, Oryza sativa (rice), photosynthesis, QTL mapping