QTLs Analysis for Five Yield-related Traits in Mungbean

doi:10.11983/CBB22108

Abstract

Abstract: The F₂ and F₃ populations derived from the cross between Sulv16-10 and Weilv11 were used to explore the genetic loci controlling yield-related traits in mungbean, and the phenotype identification and correlation analysis between yield-related traits were completed. The genetic linkage map was constructed and used for QTL analysis. The yield per plant was positively correlated with the number of pods per plant, the number of seeds per pod, hundred-seed weight and the number of branches on main stem. The correlation between the yield per plant and the number of pods per plant was the highest, and the correlation coefficient between these two traits were 0.950 and 0.914 in F₂ and F₃ population, respectively. A total of 8 QTLs for yield-related traits were detected in the F₂ population. Among them, three traits including the number of pods per plant, number of seeds per pod and yield per plant were found only on one related QTL, and each QTL accounting for 11.09% (qNPP3), 17.93% (qNSP3) and 14.18% (qYP3) of phenotype variance, respectively. Two QTLs, qBMS3 and qBMS11 related to the branch number on main stem were detected, which could explain 18.51% and 7.06% of phenotype variance, respectively. Three QTLs, qHSW3, qHSW7 and qHSW10 controlling hundred-seed weight were identified, which could explain 5.33%, 46.07% and 4.24% of phenotype variance, respectively. qNSP3 and qHSW7 were detected again in the F₃ population, demonstrating that the two QTLs were genetically stable. The InDel molecular marker R7-13.4 which closely linked to the major-effect QTL qHSW7 for hundred-seed weight was developed, and the validation of molecular marker-assisted selection was verified in natural populations. These studies provide reference for mapping, cloning of genes associated with yield-related traits as well as molecular marker-assisted selection in mungbean.

Key words: mungbean, yield-related traits, correlation analysis, QTL mapping

Weijun Ye, Yin Zhang, Peiran Wang, Lingling Zhang, Dongfeng Tian, Zejiang Wu, Bin Zhou. QTLs Analysis for Five Yield-related Traits in Mungbean[J]. Chinese Bulletin of Botany, 2023, 58(1): 150-158.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB22108

https://www.chinbullbotany.com/EN/Y2023/V58/I1/150

Figures/Tables 8

References 36

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Year	Traits	Parental lines		F₂ or F₃ population
Year	Traits	Weilv11	Sulv16-10	Means±SD	Range	CV (%)	Skewness	Kurtosis
2019 (F₂)	NPP	11.0	20.2 **	18.26±8.86	5.0-46.0	48.52	1.27	1.26
	NSP	10.1	12.0 **	10.62±0.98	9.6-14.3	7.77	-0.84	0.55
	HSW (g)	5.00	7.42 **	6.33±0.69	4.77-8.19	10.95	0.27	-0.45
	BMS	0.5	3.0 **	2.08±1.48	0.0-6.0	71.17	0.35	-0.45
	YP (g)	5.21	14.95 **	11.48±5.95	2.67-28.96	51.80	1.16	0.82
2020 (F₃)	NPP	9.4	23.2 **	16.94±8.65	5.0-48.0	51.05	1.38	2.01
	NSP	9.8	12.3 **	10.41±1.30	8.5-15.0	10.15	-0.73	0.80
	HSW (g)	5.06	7.02 **	5.47±0.68	3.40-7.20	12.51	-0.44	0.27
	BMS	0.5	3.6 **	1.71±1.39	0.0-6.0	81.68	-0.11	-1.17
	YP (g)	4.62	16.89 **	8.94±4.71	2.00-5.40	52.70	1.15	1.18

Year	Traits	Parental lines		F₂ or F₃ population
Year	Traits	Weilv11	Sulv16-10	Means±SD	Range	CV (%)	Skewness	Kurtosis
2019 (F₂)	NPP	11.0	20.2 **	18.26±8.86	5.0-46.0	48.52	1.27	1.26
	NSP	10.1	12.0 **	10.62±0.98	9.6-14.3	7.77	-0.84	0.55
	HSW (g)	5.00	7.42 **	6.33±0.69	4.77-8.19	10.95	0.27	-0.45
	BMS	0.5	3.0 **	2.08±1.48	0.0-6.0	71.17	0.35	-0.45
	YP (g)	5.21	14.95 **	11.48±5.95	2.67-28.96	51.80	1.16	0.82
2020 (F₃)	NPP	9.4	23.2 **	16.94±8.65	5.0-48.0	51.05	1.38	2.01
	NSP	9.8	12.3 **	10.41±1.30	8.5-15.0	10.15	-0.73	0.80
	HSW (g)	5.06	7.02 **	5.47±0.68	3.40-7.20	12.51	-0.44	0.27
	BMS	0.5	3.6 **	1.71±1.39	0.0-6.0	81.68	-0.11	-1.17
	YP (g)	4.62	16.89 **	8.94±4.71	2.00-5.40	52.70	1.15	1.18

Traits	NPP	NSP	HSW	BMS
NSP	0.362 **
NSP	0.357 **
HSW	-0.005	-0.049
HSW	0.149	0.125
BMS	0.403 **	0.190 *	0.174 *
BMS	0.708 **	0.423 **	0.311 **
YP	0.950 **	0.409 **	0.163	0.425 **
YP	0.914 **	0.464 **	0.318 **	0.701 **

Traits	NPP	NSP	HSW	BMS
NSP	0.362 **
NSP	0.357 **
HSW	-0.005	-0.049
HSW	0.149	0.125
BMS	0.403 **	0.190 *	0.174 *
BMS	0.708 **	0.423 **	0.311 **
YP	0.950 **	0.409 **	0.163	0.425 **
YP	0.914 **	0.464 **	0.318 **	0.701 **

Year	Traits	QTL name	Chromosome	Marker interval	Position (cM)	Likelihood of odd (LOD)	Add effect	Phenotype variance explained (%)
2019	NPP	qNPP3	3	ID3-8-ID3-9	30.60-31.34	3.47	4.62	11.09
	NSP	qNSP3	3	ID3-5-ID3-6	13.16-23.40	5.92	0.58	17.93
	HSW	qHSW3	3	ID3-7-ID3-9	29.12-31.34	3.78	0.26	5.33
		qHSW7	7	ID7-10-ID7-7	100.12-103.50	23.15	0.71	46.07
		qHSW10	10	ID10-3-ID10-4	6.84-9.08	2.90	-0.09	4.24
	BMS	qBMS3	3	ID3-8-ID3-9	30.60-31.34	6.44	1.03	18.51
	BMS	qBMS11	11	ID11-6-ID11-7	33.46-35.32	2.85	0.25	7.06
	YP	qYP3	3	ID3-8-ID3-9	30.60-31.34	4.60	3.67	14.18
2020	NPP	qNPP3_a	3	ID3-4-ID3-5	9.83-13.06	3.39	3.63	2.27
	NSP	qNSP3_a	3	R3-9-R3-12	20.88-22.23	6.37	0.66	19.93
	HSW	qHSW7_a	7	ID7-10-ID7-7	29.77-31.76	8.80	0.42	30.17