Chinese Bulletin of Botany ›› 2009, Vol. 44 ›› Issue (02): 236-244.DOI: 10.11983/CBB28038  cstr: 32102.14.j.issn.1674-3466.2009.02.014

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Research in In Planta Transformation Methods and Their Applications and Prospects in Brassica napus

Zhi Zou, Changming Lu*   

  1. Laboratory of Genetic Engineering and Genetically Modified Organism Biosafety, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Received:2008-02-20 Revised:2008-04-25 Online:2009-03-01 Published:2009-03-01
  • Contact: Changming Lu

Abstract:

In planta transformation methods for obtaining transgenic plants without de-differentiation and redifferentiation of receptor cells have been explored bec-ause of their advantages of genotype independence, ease of operation, high efficiency, lack of somatic mutation, and genetic stability through future gener-ations. Most methods use meristematic tissues or reproductive cells as recept-ors. This review focuses on the current progress of in planta transformation methods, with an emphasis on their applications and prospects for genetic transformation and functional genomics research in Brassica napus.

Key words: Agrobacterium tumefaciens, Brassica napus, in planta transformation methods