Chinese Bulletin of Botany ›› 2024, Vol. 59 ›› Issue (1): 54-65.DOI: 10.11983/CBB23076 cstr: 32102.14.CBB23076
• EXPERIMENTAL COMMUNICATIONS • Previous Articles Next Articles
Yi Song, Hanghang Chen, Xin Cui, Zhifeng Lu, Shipeng Liao, Yangyang Zhang, Xiaokun Li, Rihuan Cong, Tao Ren*(), Jianwei Lu
Received:
2023-06-11
Accepted:
2023-11-02
Online:
2024-01-10
Published:
2024-01-10
Contact:
*E-mail: Yi Song, Hanghang Chen, Xin Cui, Zhifeng Lu, Shipeng Liao, Yangyang Zhang, Xiaokun Li, Rihuan Cong, Tao Ren, Jianwei Lu. Potassium Nutrient Status-mediated Leaf Growth of Oilseed Rape (Brassica napus) and Its Effect on Phyllosphere Microorganism[J]. Chinese Bulletin of Botany, 2024, 59(1): 54-65.
Treatments | K content (%) | Dry matter weight (g) | Leaf area (cm2) | Specific leaf weight (mg∙cm‒2) | Waxy content (µg∙cm‒2) | Stomatal density (numbers∙cm‒2) | Active protein content (%) | Soluble sugar content (%) | Saccharose content (%) | Fructose content (%) | Starch content (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
K0 | 0.35 c | 1.09 b | 335 b | 4.2 a | 223 ab | 1630 a | 14.6 a | 8.9 c | 4.3 b | 5.1 c | 16.3 b |
K30 | 0.61 b | 1.33 b | 382 b | 4.4 a | 306 a | 1449 a | 14.5 a | 15.9 b | 7.3 a | 10.9 b | 20.6 b |
K180 | 0.92 a | 1.86 a | 508 a | 4.4 a | 188 b | 970 b | 16.3 a | 20.3 a | 9.4 a | 14.2 a | 25.9 a |
Table 1 The phenotypic traits and biochemical parameters of oilseed rape leaves with different K nutrient status
Treatments | K content (%) | Dry matter weight (g) | Leaf area (cm2) | Specific leaf weight (mg∙cm‒2) | Waxy content (µg∙cm‒2) | Stomatal density (numbers∙cm‒2) | Active protein content (%) | Soluble sugar content (%) | Saccharose content (%) | Fructose content (%) | Starch content (%) |
---|---|---|---|---|---|---|---|---|---|---|---|
K0 | 0.35 c | 1.09 b | 335 b | 4.2 a | 223 ab | 1630 a | 14.6 a | 8.9 c | 4.3 b | 5.1 c | 16.3 b |
K30 | 0.61 b | 1.33 b | 382 b | 4.4 a | 306 a | 1449 a | 14.5 a | 15.9 b | 7.3 a | 10.9 b | 20.6 b |
K180 | 0.92 a | 1.86 a | 508 a | 4.4 a | 188 b | 970 b | 16.3 a | 20.3 a | 9.4 a | 14.2 a | 25.9 a |
Figure 2 Correlation between leaf phenotypic traits and biochemical parameters under different K nutrient status *, ** and *** indicate significant differences at 0.05, 0.01 and 0.001 levels, respectively.
Figure 3 Diversity of phyllospheric bacterial communities in oilseed rape under different K nutrient status (A) Pielou_evenness, Richness and Shannon diversity indices of phyllospheric bacterial communities (different lowercase letters indicate significant differences among treatments (P<0.05)); (B) β-diversity of phyllospheric bacterial communities; (C) Distribution of phyllospheric microbial communities Amplicon Sequence Variants (ASVs). NMDS: Evaluation of rank information for distance values; K0, K30, and K180 are the same as shown in Figure 1.
Figure 4 Phyllospheric microbial composition in leaves of oilseed rape under different K nutrient status (A) Phyllospheric microbial composition of oilseed rape at phylum level; (B) Distribution of species at the taxonomic level of families under different K nutrient status (circles and font sizes are mapped based on relative abundance, colored by phylum taxonomic level). K0, K30, and K180 are the same as shown in Figure 1.
Figure 5 Phyllospheric microbial co-occurrence network and node and node relative abundance relationships for different K nutrient status K0, K30, and K180 are the same as shown in Figure 1.
Treatments | Nodes | Edges | Network density | Modularity |
---|---|---|---|---|
K0 | 100 | 789 | 0.159 | 0.650 |
K30 | 138 | 390 | 0.041 | 0.912 |
K180 | 174 | 536 | 0.036 | 0.915 |
Table 2 Phyllospheric microbial co-occurrence network parameters for different K nutrient status
Treatments | Nodes | Edges | Network density | Modularity |
---|---|---|---|---|
K0 | 100 | 789 | 0.159 | 0.650 |
K30 | 138 | 390 | 0.041 | 0.912 |
K180 | 174 | 536 | 0.036 | 0.915 |
Figure 6 Relationship between leaf traits and phyllospheric microbial composition in oilseed rape under different K nutrient status (A) Mental relationship between leaf phenotypic nutrition and phyllospheric microbial community structure (* P<0.05; ** P<0.01; *** P<0.001); (B) RDA analysis under different potassium nutrient conditions; (C) Correlation analysis between dominant species and leaf phenotypic traits; (D) Relative contributions of leaf structure and leaf fractions to phyllospheric microbial community changes in oilseed rape. CCA: The most important components affect changes in the phyllospheric microbial community; K0, K30, and K180 are the same as shown in Figure 1.
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