Effects of Sucrose on Seedling Growth and Development and SnRK1 Activity in Prunus persica

doi:10.11983/CBB19032

Abstract

Abstract: This study analyzed the SnRK1 activity and seedling growth and development in response to different concentrations and durations of exogenous sucrose in peach (Prunus persica), thus providing theoretical basis and technical support for fruit production. The data showed that 5% sucrose treatment greatly enhanced the SnRK1 activity, which was continuously increased within a certain period of treatment. Compared with water and mannitol controls, 5% sucrose treatment significantly increased the contents of soluble sugar, starch, and chlorophyll, the biomass of aboveground and underground, and the net photosynthetic rate. Examination of root configuration showed that 5% sucrose significantly increased root surface area, root volume, and number of lateral roots, and promoted root thickening and elongation. qRT-PCR analysis showed that exogenous sucrose promoted auxin synthesis and transport in roots. In conclusion, application of certain concentration of sucrose promotes SnRK1 activity, carbon metabolism, and plant growth and development, increases root auxin synthesis and transport and affects root configuration in peach.

Key words: peach, SnRK1, sucrose, auxin, root configuration

Shuhui Zhang,Hong Wang,Wenru Wang,Xuelian Wu,Yuansong Xiao,Futian Peng. Effects of Sucrose on Seedling Growth and Development and SnRK1 Activity in Prunus persica[J]. Chinese Bulletin of Botany, 2019, 54(6): 744-752.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB19032

https://www.chinbullbotany.com/EN/Y2019/V54/I6/744

Figures/Tables 10

Table 1 Primers used in this study

Gene	Primer name	Primer sequence (5′-3′)
PpPIN1 (Ppa002944m)	P-PIN1-F	TAACAATACGACAGCGCATTACC
PpPIN1 (Ppa002944m)	P-PIN1-R	TGAAGATCCTTACCACCATCCTC
PpPIN2 (Ppa024134m)	P-PIN2-F	TTCGAATCTCACGGGAGTGG
PpPIN2 (Ppa024134m)	P-PIN2-R	GAATCCACCTTGGAAACTGTTTG
PpPIN3 (Ppa002528m)	P-PIN3-F	ATCTAACCTTACAGGCGCAGAGA
PpPIN3 (Ppa002528m)	P-PIN3-R	GAGTCTCTTCGAAATTTGACGGT
PpYUC2 (Ppa022204m)	P-YUC2-F	GACCCAGCAGTGTTCGATCA
PpYUC2 (Ppa022204m)	P-YUC2-R	CTGCCTCCTCCAATTCTGGCT
PpYUC6 (Ppa005244m)	P-YUC6-F	TCCTCCTCATCACCATCACA
PpYUC6 (Ppa005244m)	P-YUC6-R	CCACAAGAGGCTATGCAATT
PpActin	P-Actin-F	GTTATTCTTCATCGGCGTCTTCG
PpActin	P-Actin-R	CTTCACCATTCCAGTTCCATTGTC

Figure 1 Effects of different sucrose concentrations on SnRK1 activity in peach seedlings S1: Water; S2: 1% sucrose solution; S3: 3% sucrose solution; S4: 5% sucrose solution; S5: 7% sucrose solution. Different lowercase letters above the bars indicate significant differences among different treatmens (P<0.05).

Figure 2 Effects of sucrose treatment on SnRK1 activity in peach seedlings T1: Water; T2: 5% sucrose solution; T3: 5% mannitol solution. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

Figure 3 Effects of exogenous sucrose on soluble sugar contents in leaves (A) and roots (B) of peach seedlings T1-T3 see Ffddigure 2. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

Figure 4 Effects of exogenous sucrose on starch contents in leaves (A) and roots (B) of peach seedlings T1-T3 see Ffddigure 2. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

Figure 5 Effects of exogenous sucrose on relative content of chlorophyll (A) and photosynthetic rate (B) of peach leaves T1-T3 see Ffddigure 2. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

Figure 6 Effects of exogenous sucrose on fresh weight of peach seedlings T1-T3 see Ffddigure 2. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

Table 2 Effects of exogenous sucrose on the root architecture of peach seedlings

Experimental treatments	Number of primary lateral roots	Number of secondary lateral roots	Total root length (cm)	Total root surface area (cm²)
T1	235.00±14.42 b	123.67±7.02 b	86.63±6.01 b	9.82±1.00 b
T2	417.33±14.47 a	338.00±12.77 a	147.66±14.99 a	17.82±1.49 a
T3	107.67±8.96 c	62.67±7.64 c	67.67±3.71 c	6.75±0.54 c

Figure 7 Effects of exogenous sucrose on roots phenotype of peach seedlings T1-T3 see Ffddigure 2. Bar=3 cm

Figure 8 Effects of exogenous sucrose on auxin content (A) and gene expression (B-F) in peach seedling roots T1-T3 see Ffddigure 2. Different lowercase letters above the bars indicate significant differences among different treatments (P<0.05).

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