Chinese Bulletin of Botany ›› 2022, Vol. 57 ›› Issue (5): 623-634.DOI: 10.11983/CBB22048

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Effect of Nitrogen-doped Graphene Quantum Dots on Growth Direction of Primary Root in Arabidopsis thaliana

Ye Qing, Yan Xiaoyan, Chen Huize, Feng Jinlin, Han Rong*()   

  1. Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response in Shanxi Province, School of Life Science, Shanxi Normal University, Taiyuan 030031, China
  • Received:2022-03-14 Accepted:2022-06-28 Online:2022-09-01 Published:2022-09-09
  • Contact: Han Rong
  • About author:*E-mail:

Abstract: Graphene quantum dots (GQDs) have substantial application potentials in various fields such as electrochemical biosensor, bioimaging and biomedicine. Therefore, with the increasing exposure to the public and environment, their biosafety has aroused increasing concerns in recent years. So far, the influence of GQDs on the growth and development of plants is still poorly understood. In this study, we investigated the influence of nitrogen-doped GQDs (N-GQDs) treatment on the growth direction of primary root in Arabidopsis thaliana at cellular and molecular levels. We found that the N-GQDs were absorbed by roots and transported via vascular bundles. After the N-GQDs treatment at the concentration of 50-100 mg∙L-1, the growth direction of primary roots was changed, curving towards the outside of the culture medium. Because of the N-GQDs treatment, the starch granule accumulation of columnar cells was reduced, the abundance of auxin efflux carrier PIN3 was repressed, and the PIN3 in columnar cells was relocated to outer lateral membrane distant from the culture medium (towards the air), which resulted in the asymmetric auxin distribution in the root tips and the curved growth of primary roots towards a direction distant from the medium in order to run away from the high-concentration N-GQDs environment. The study results provide a reference direction for exploring the possible coping mechanism of plants with N-GQDs treatment, and also provide corresponding data for biosafety evaluation of N-GQDs.

Key words: auxin, nitrogen-doped graphene quantum dots, PIN3, root growth, starch granule