Microarray Analysis of Genes Expressed in Brassica napus Stigma

doi:10.3724/SP.J.1259.2014.00246

Abstract

Abstract: Genes expressed in stigma were screened for a pollination-deficient mutant FS-M1 in Brassica napus and its wild type (NY10) by using B. napus gene microarray. Among the 16 540 genes (43 803 probes) represented on the array, 4 410 showed significant differential expression; the differential expression of selected genes was validated by qRT-PCR. We identified 198 genes (209 probes) that were significantly upregulated in the wild-type stigma and were markedly enriched in function of hydrolase activity, transferase activity, oxidoreductase activity and transcription factors, and some considerable gene families were involved, including cytochrome P450, GDSL-motif lipase, ABC transporter, myb transcription factor, bHLH transcription factor, peroxidase and receptor kinase, which may be related to development and pollination of B. napus stigma.

Key words: Brassica napus, gene expression profiling, gene microarray, qRT-PCR, stigma

Chunhong Li, Sanxiong Fu, Cunkou Qi. Microarray Analysis of Genes Expressed in Brassica napus Stigma[J]. Chinese Bulletin of Botany, 2014, 49(3): 246-253.

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URL: https://www.chinbullbotany.com/EN/10.3724/SP.J.1259.2014.00246

https://www.chinbullbotany.com/EN/Y2014/V49/I3/246

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