Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (6): 713-722.DOI: 10.11983/CBB16239

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Functional Analysis of Brassica napus BnTTG1-1 Gene

Liu Kaige, Qi Shuanghui, Duan Shaowei, Li Dong, Jin Changyu, Gao Chenhao, Liu Mingxun Chen Xuanxia*()   

  1. College of Agronomy, Northwest A&F University, Yangling 712100, China
  • Received:2016-12-05 Accepted:2017-04-03 Online:2017-11-01 Published:2017-11-22
  • Contact: Liu Mingxun Chen Xuanxia

Abstract: AtTTG1 existing in the nucleus as a WD40 repeat transcription factor plays important roles in regulating trichome initiation, anthocyanin biosynthesis, and storage reserve accumulation in Arabidopsis thaliana. In the present study, we cloned the full-length coding domain sequence (CDS) of the BnTTG1-1 gene from the Brassica napus cv. ‘QINYOU Seven’, analyzed its subcellular localization, detected its temporal and spatial expression patterns in different tissues, and investigated its functions in several biological processes. BnTTG1-1 was localized in the nucleus of tobacco leaf cells, so it may function as a transcription factor. BnTTG1-1 was widely expressed in various vegetative tissues and developing seeds in QINYOU Seven. Moreover, introducing 35S:BnTTG1-1 into the mutant ttg1-13 fully rescued many phenotypes of the mutant, such as no trichomes and anthocyanins, yellow seed coat, higher contents of seed fatty acids and storage proteins, and sensitivity to higher sucrose or salinity stresses during seed germination and seedling establishment. Thus, BnTTG1-1 and AtTTG1 exhibited conserved functions on many biological processes during plant growth and development.

Key words: TTG1, Brassica napus, Arabidopsis thaliana, functional complementation