Mechanism of AtCPL1 in Regulating Flowering of Arabidopsis

doi:10.11983/CBB14188

Abstract

Abstract: CTD-phosphatase-like 1 (CPL1), the RNA polymerase II, plays an important role in plant stress responses, ion absorption and floral induction by regulating the phosphorylation of RNA polymerase II. To study the mechanism of CPL1 involved in regulation of flowering, two AtCPL1 mutants, cpl1-3 and fry2-1, were used for observing differences in flowering phenotype. Deletion of AtCPL1 caused flowering delay in Arabidopsis under long-day conditions. Moreover, the expression of genes related to flowering regulatory pathways in cpl1-3 and fry2-1 mutants were analyzed by qRT-PCR. The expression of FT, FD, CO and miR172 was significantly upregulated in cpl1-3 and fry2-1 mutants, but that of miR156a, TOEs and SMZ was significantly downregulated. AtCPL1 may be involved in the regulation of flowering in Arabidopsis as a positive regulatory factor, perhaps by regulating the expression of miR156 and miR172, which affect the expression of flowering-related genes.

Key words: AtCPL1, Arabidopsis thaliana, flowering, flowering-regulated pathway

Yuying Qi, Yanli Zhan, Cuibo Wang, Fadi Chen, Jiafu Jiang. Mechanism of AtCPL1 in Regulating Flowering of Arabidopsis[J]. Chinese Bulletin of Botany, 2016, 51(1): 9-15.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB14188

https://www.chinbullbotany.com/EN/Y2016/V51/I1/9

Figures/Tables 4

References 32

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Primer name	Sequence (5'-3')	Annotation
cpl1-3-F	TTGTCCTCATATTTGTGAGTTTTGG
cpl1-3-R	CCTCAAATGAGCGCATGGTATT
fry2-1- F	CTCAACTCAGTCTGACAGGATGCT
fry2-1-R	TCCCAATACCCAAGTCAGTGATT
UBQ10-F	GGCCTTGTATAATCCCTGATGAATAAG	Liu et al., 2011
UBQ10-R	AAAGAGATAACAGGAACGGAAACATAGT	Liu et al., 2011
FT-F	AGACGTTCTTGATCCGTTTA	Jung et al., 2007
FT-R	GTAGATCTCAGCAAACTCGC	Jung et al., 2007
CO-F	CCGGGTCTGCGAGTCATG	Yu et al., 2012
CO-R	GGCATCATCTGCCTCACACA	Yu et al., 2012
SOC1-F	GGTTTCTGAAGAAAATATGCAGCATTTG	Yang et al., 2010
SOC1-R	GGCTACTCTCTTCATCACCTCTTCCAG	Yang et al., 2010
FD-F	GCGCTAGGAAACAGGAATGCTTATACAAA	Yang et al., 2010
FD-R	AGCTGTGGAAGACCGTTGAAGTGTG	Yang et al., 2010
SMZ-F	AGGGAGAAGGAGCCATGAAGTTTGGTG	Yu et al., 2012
SMZ-R	GTCTTCAGAGGTTTCATGGTTGCCATG	Yu et al., 2012
miR156a-F	CTCAAGTTCATTGCCATTTTTAGG	Wang et al., 2009
miR156a-R	GAGAGATTGAGACATAGAGAACGAAGA	Wang et al., 2009
miR172a1-F	GGGTTTGAGTTTGAGTTTGA	Jung et al., 2007
miR172a1-R	TGGATGGAATCCAAATAATC	Jung et al., 2007
miR172a2-F	TCGGCGGATCCATGGAAGAAAGCTC	Jung et al., 2007
miR172a2-R	TTTCTCAAGCTTTAGGTATTTGTAG	Jung et al., 2007
miR172b1-F	CCGTCTTGAGTCTTGAAAAG	Jung et al., 2007
miR172b1-R	GAAATACCTCCGATCTGTGA	Jung et al., 2007
TOE1-F	CGAGTTATAATAATCCCGCCGAG	Yu et al., 2012
TOE1-R	TTAAGGGTGTGGATAAAAGT	Yu et al., 2012
TOE2-F	ATGGAGAACCACATGGCTGC	Yu et al., 2012
TOE2-R	GGTGCTGTAGCTGCTACGGC	Yu et al., 2012
TOE3-F	ACGAGGAACGGTCATAATCTTG	Yu et al., 2012
TOE3-R	ATTCCCACCACTCGATTTCCT	Yu et al., 2012
FLC-F	AGCCAAGAAGACCGAACTCA	Yu et al., 2012
FLC-R	TTTGTCCAGCAGGTGACATC	Yu et al., 2012

Primer name	Sequence (5'-3')	Annotation
cpl1-3-F	TTGTCCTCATATTTGTGAGTTTTGG
cpl1-3-R	CCTCAAATGAGCGCATGGTATT
fry2-1- F	CTCAACTCAGTCTGACAGGATGCT
fry2-1-R	TCCCAATACCCAAGTCAGTGATT
UBQ10-F	GGCCTTGTATAATCCCTGATGAATAAG	Liu et al., 2011
UBQ10-R	AAAGAGATAACAGGAACGGAAACATAGT	Liu et al., 2011
FT-F	AGACGTTCTTGATCCGTTTA	Jung et al., 2007
FT-R	GTAGATCTCAGCAAACTCGC	Jung et al., 2007
CO-F	CCGGGTCTGCGAGTCATG	Yu et al., 2012
CO-R	GGCATCATCTGCCTCACACA	Yu et al., 2012
SOC1-F	GGTTTCTGAAGAAAATATGCAGCATTTG	Yang et al., 2010
SOC1-R	GGCTACTCTCTTCATCACCTCTTCCAG	Yang et al., 2010
FD-F	GCGCTAGGAAACAGGAATGCTTATACAAA	Yang et al., 2010
FD-R	AGCTGTGGAAGACCGTTGAAGTGTG	Yang et al., 2010
SMZ-F	AGGGAGAAGGAGCCATGAAGTTTGGTG	Yu et al., 2012
SMZ-R	GTCTTCAGAGGTTTCATGGTTGCCATG	Yu et al., 2012
miR156a-F	CTCAAGTTCATTGCCATTTTTAGG	Wang et al., 2009
miR156a-R	GAGAGATTGAGACATAGAGAACGAAGA	Wang et al., 2009
miR172a1-F	GGGTTTGAGTTTGAGTTTGA	Jung et al., 2007
miR172a1-R	TGGATGGAATCCAAATAATC	Jung et al., 2007
miR172a2-F	TCGGCGGATCCATGGAAGAAAGCTC	Jung et al., 2007
miR172a2-R	TTTCTCAAGCTTTAGGTATTTGTAG	Jung et al., 2007
miR172b1-F	CCGTCTTGAGTCTTGAAAAG	Jung et al., 2007
miR172b1-R	GAAATACCTCCGATCTGTGA	Jung et al., 2007
TOE1-F	CGAGTTATAATAATCCCGCCGAG	Yu et al., 2012
TOE1-R	TTAAGGGTGTGGATAAAAGT	Yu et al., 2012
TOE2-F	ATGGAGAACCACATGGCTGC	Yu et al., 2012
TOE2-R	GGTGCTGTAGCTGCTACGGC	Yu et al., 2012
TOE3-F	ACGAGGAACGGTCATAATCTTG	Yu et al., 2012
TOE3-R	ATTCCCACCACTCGATTTCCT	Yu et al., 2012
FLC-F	AGCCAAGAAGACCGAACTCA	Yu et al., 2012
FLC-R	TTTGTCCAGCAGGTGACATC	Yu et al., 2012

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