脱落酸对水稻抽穗开花期高温胁迫的诱抗效应

doi:10.11983/CBB22022

植物学报 ›› 2022, Vol. 57 ›› Issue (5): 596-610.DOI: 10.11983/CBB22022

脱落酸对水稻抽穗开花期高温胁迫的诱抗效应

刘晓龙¹^,²^,^*(), 季平¹, 杨洪涛¹^,², 丁永电¹^,², 付佳玲¹, 梁江霞¹, 余聪聪¹

¹宜春学院, 宜春 336000
²江西省高等学校硒农业工程技术研究中心, 宜春 336000

收稿日期:2022-01-28 接受日期:2022-04-20 出版日期:2022-09-01 发布日期:2022-09-09
通讯作者: 刘晓龙
作者简介:*E-mail: lxl032202@163.com
基金资助:
江西省教育厅科学技术研究项目(GJJ190868);江西省自然科学基金(20202BABL213046)

Priming Effect of Abscisic Acid on High Temperature Stress During Rice Heading-flowering Stage

Liu Xiaolong¹^,²^,^*(), Ji Ping¹, Yang Hongtao¹^,², Ding Yongdian¹^,², Fu Jialing¹, Liang Jiangxia¹, Yu Congcong¹

¹Yichun University, Yichun 336000, China
²Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, Yichun 336000, China

Received:2022-01-28 Accepted:2022-04-20 Online:2022-09-01 Published:2022-09-09
Contact: Liu Xiaolong
About author:*E-mail: lxl032202@163.com

摘要/Abstract

摘要：

为探究脱落酸(ABA)对水稻(Oryza sativa)抽穗开花期高温胁迫的诱抗效应, 以江西省主推水稻品种黄华占为材料, 于孕穗期用蒸馏水、ABA溶液(10、50和100 µmol∙L^-1)、氟啶酮(FLU)和原花青素(PC) 6种溶液进行叶面喷施, 然后移入对照(CK)和高温胁迫(HS)环境处理8天, 考查籽粒活性氧(ROS)积累、抗氧化防御能力、产量构成及相关基因的表达。结果表明, 高温胁迫下, 水稻的穗长、穗重、结实率、千粒重和产量与超氧阴离子和过氧化氢含量呈显著负相关。高温胁迫下, 喷施ABA显著上调了ABA应答和抗氧化防御基因的表达, 籽粒中活性氧含量下降了8.24%-31.35%; 喷施ABA显著增加了水稻的穗长、穗重、结实率和千粒重, 显著上调了产量形成基因的表达, 增产12.73%-20.77%。高温胁迫下, 喷施FLU可抑制ABA的生物合成, 导致ROS过量积累和水稻减产; 喷施抗氧化剂PC则抑制ROS过量积累, 使产量增加。以上结果表明, 高温胁迫下, 孕穗期喷施ABA不仅能够激发ABA信号通路, 而且上调抗氧化防御能力和产量形成基因的表达, 进而提高水稻在抽穗开花期的耐热性, 达到增产目的。

关键词: 水稻, 脱落酸, 高温胁迫, 活性氧, 产量

Abstract:

To understand the priming effect of abscisic acid (ABA) on high temperature stress during rice heading-flowering stage, Huanghuazhan, a main rice variety in Jiangxi province, was used as experimental material. Six kinds of solutions were arranged to spray on rice at the booting stage, including distilled water, ABA solution (10, 50 and 100 µmol∙L^-1), fluridone (FLU) and proanthocyanidins (PC), then the rice plants were moved to control (CK) and high temperature stress (HS) conditions for 8 d. The effects of ABA by spraying at booting stage were measured by the accumulation of reactive oxygen species (ROS), antioxidant defense capacity, yield components and the expression levels of related genes. The results showed that significant negative correlations were detected between the panicle length, panicle weight, percentage of filled spikelets, 1 000-kernel weight, grain yield and contents of superoxide anion and hydrogen peroxide respectively. Spraying ABA solution significantly upregulated the expression of ABA-responsive and antioxidant defense genes, and decreased contents of ROS by 8.24%-31.35% under high temperature stress. The panicle length, panicle weight, percentage of filled spikelets and 1 000-kernel weight were significantly increased by spraying ABA solution, as well as the expression levels of yield related genes. The grain yield was increased by 12.73%-20.77% by spraying ABA solution under high temperature stress. The application of the ABA biosynthesis inhibitor, FLU, inhibited the ABA biosynthesis and leaded to excessive accumulation of ROS and yield loss under high temperature stress. While application of the antioxidant PC inhibited the excessive accumulation of ROS and improved grain yield under high temperature stress. These results suggested that spraying ABA solution at booting stage enhanced tolerance to high temperature stress in rice at heading-flowering stage by upregulating not only ABA signaling pathway, but also antioxidant defense activity and yield related genes, so as to further improve grain yield under high temperature stress.

Key words: rice (Oryza sativa), abscisic acid, high temperature stress, reactive oxygen species, yield

刘晓龙, 季平, 杨洪涛, 丁永电, 付佳玲, 梁江霞, 余聪聪. 脱落酸对水稻抽穗开花期高温胁迫的诱抗效应. 植物学报, 2022, 57(5): 596-610.

Liu Xiaolong, Ji Ping, Yang Hongtao, Ding Yongdian, Fu Jialing, Liang Jiangxia, Yu Congcong. Priming Effect of Abscisic Acid on High Temperature Stress During Rice Heading-flowering Stage. Chinese Bulletin of Botany, 2022, 57(5): 596-610.

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB22022

https://www.chinbullbotany.com/CN/Y2022/V57/I5/596

图/表 9

参考文献 51

[1]	陈唯, 曾晓贤, 谢楚萍, 田长恩, 周玉萍 (2019). 植物内源ABA水平的动态调控机制. 植物学报 54, 677-687.
[2]	段骅, 傅亮, 剧成欣, 刘立军, 杨建昌 (2013). 氮素穗肥对高温胁迫下水稻结实和稻米品质的影响. 中国水稻科学 27, 591-602.
[3]	郭贵华, 刘海艳, 李刚华, 刘明, 李岩, 王绍华, 刘正辉, 唐设, 丁艳锋 (2014). ABA缓解水稻孕穗期干旱胁迫生理特性的分析. 中国农业科学 47, 4380-4391.
[4]	雷娅伟, 白小明, 王婷, 吕优伟, 雷舒涵 (2015). 脱落酸对3个野生草地早熟禾种质高温胁迫的缓解效应. 草地学报 23, 89-94, 100.
[5]	李迎春, 帅细强, 杨蓉, 刘丹 (2019). 高温热害对江西省早稻产量影响的定量分析. 湖北农业科学 58(20), 79-83.
[6]	刘晓龙, 季平, 杨洪涛, 邵勤, 任珺怡, 祝佳滢, 张超毅, 陆晨旭 (2021). 高温胁迫对水稻内源脱落酸含量及相关基因表达的影响. 分子植物育种 1-12. [2021-12-20].https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=FZZW20211215000&uniplatform=NZKPT&v=_MB1PS_Ch2BhePRwLHu5Uh4VVaM4GNe8EyhVd2rEe7ln_AJgZxEiEWU6WlC2bx-z.
[7]	隆春艳, 古洪辉, 汪正香, 蒋雄, 杨翠芹, 秦耀国 (2017). 外源脱落酸对高温胁迫下菠菜光合与叶绿素荧光参数的影响. 四川农业大学学报 35, 24-30.
[8]	王强, 陈雷, 张晓丽, 唐茂艳, 吕荣华, 陶伟, 梁天锋 (2015). 化学调控对水稻高温热害的缓解作用研究. 中国稻米 21 (4), 80-82. DOI
[9]	杨建莹, 霍治国, 王培娟, 邬定荣 (2020). 江西早稻高温热害等级动态判识及时空变化特征. 应用生态学报 31, 199-207.
[10]	杨卫丽, 黄福灯, 曹珍珍, 雷炳婷, 胡东维, 程方民 (2013). 高温胁迫对水稻光合PSII系统伤害及其与叶绿体D1蛋白间关系. 作物学报 39, 1060-1068.
[11]	杨雲雲, 陈鑫, 陈启洲, 卢芳, 徐晨, 杨洪涛, 苏佩佩, 刘晓龙 (2021). 脱落酸对水稻种子萌发期耐高温胁迫的诱抗效应. 华北农学报 36(3), 185-194. DOI
[12]	张桂莲, 陈立云, 张顺堂, 肖应辉, 贺治洲, 雷东阳 (2006). 高温胁迫对水稻剑叶保护酶活性和膜透性的影响. 作物学报 32, 1306-1310.
[13]	Brennan T, Frenkel C (1977). Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol 59, 411-416.
[14]	Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017). Reactive oxygen species, abiotic stress and stress combination. Plant J 90, 856-867.
[15]	Dar NA, Amin I, Wani W, Wani SA, Shikari AB, Wani SH, Masoodi KZ (2017). Abscisic acid: a key regulator of abiotic stress tolerance in plants. Plant Gene 11, 106-111.
[16]	Dwivedi SK, Basu S, Kumar S, Kumari S, Kumar A, Jha S, Mishra JS, Bhatt BP, Kumar G (2019). Enhanced antioxidant enzyme activities in developing anther contributes to heat stress alleviation and sustains grain yield in wheat. Funct Plant Biol 46, 1090-1102.
[17]	Elstner EF, Heupel A (1976). Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Anal Biochem 70, 616-620.
[18]	Feng BH, Zhang CX, Chen TT, Zhang XF, Tao LX, Fu GF (2018). Salicylic acid reverses pollen abortion of rice caused by heat stress. BMC Plant Biol 18, 245.
[19]	Fu GF, Feng BH, Zhang CX, Yang YJ, Yang XQ, Chen TT, Zhao X, Zhang XF, Jin QY, Tao LX (2016). Heat stress is more damaging to superior spikelets than inferiors of rice (Oryza sativa L.) due to their different organ temperatures. Front Plant Sci 7, 1637.
[20]	Hirose T, Ohdan T, Nakamura Y, Terao T (2006). Expression profiling of genes related to starch synthesis in rice leaf sheaths during the heading period. Physiol Plant 128, 425-435.
[21]	Huang J, Zhang FM, Xue Y, Lin J (2017). Recent changes of rice heat stress in Jiangxi province, southeast China. Int J Biometeorol 61, 623-633.
[22]	Huang YC, Niu CY, Yang CR, Jinn TL (2016). The heat stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiol 172, 1182-1199.
[23]	Janni M, Gullì M, Maestri E, Marmiroli M, Valliyodan B, Nguyen HT, Marmiroli N (2020). Molecular and genetic bases of heat stress responses in crop plants and breeding for increased resilience and productivity. J Exp Bot 71, 3780-3802.
[24]	Jiang CJ, Liu XL, Liu XQ, Zhang H, Yu YJ, Liang ZW (2017). Stunted growth caused by blast disease in rice seedlings is associated with changes in phytohormone signaling pathways. Front Plant Sci 8, 1558.
[25]	Joshee N, Kisaka H, Kitagawa Y (1998). Isolation and characterization of a water stress-specific genomic gene, pwsi 18, from rice. Plant Cell Physiol 39, 64-72.
[26]	Li N, Euring D, Cha JY, Lin Z, Lu MZ, Huang LJ, Kim WY (2021). Plant hormone-mediated regulation of heat tolerance in response to global climate change. Front Plant Sci 11, 627969.
[27]	Liu XL, Xie XZ, Zheng CK, Wei LX, Li XW, Jin YY, Zhang GH, Jiang CJ, Liang ZW (2022). RNAi-mediated suppression of the abscisic acid catabolism gene OsABA- 8ox1 increases abscisic acid content and tolerance to saline-alkaline stress in rice (Oryza sativa L.). Crop J 10, 354-367.
[28]	Liu XL, Zhang H, Jin YY, Wang MM, Yang HY, Ma HY, Jiang CJ, Liang ZW (2019). Abscisic acid primes rice seedlings for enhanced tolerance to alkaline stress by upregulating antioxidant defense and stress tolerance- related genes. Plant Soil 438, 39-55.
[29]	Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method. Methods 25, 402-408.
[30]	Qiao B, Zhang Q, Liu DL, Wang HQ, Yin JY, Wang R, He ML, Cui M, Shang ZL, Wang DK, Zhu ZG (2015). A calcium-binding protein, rice annexin OsANN1, enhances heat stress tolerance by modulating the production of H₂O₂. J Exp Bot 66, 5853-5866.
[31]	Qin P, Zhang GH, Hu BH, Wu J, Chen WL, Ren ZJ, Liu YL, Xie J, Yuan H, Tu B, Ma BT, Wang YP, Ye LM, Li LG, Xiang CB, Li SG (2021). Leaf-derived ABA regulates rice seed development via a transporter-mediated and temperature-sensitive mechanism. Sci Adv 7, eabc8873.
[32]	Qiu ZN, Zhu L, He L, Chen DD, Zeng DL, Chen G, Hu J, Zhang GH, Ren DY, Dong GJ, Gao ZY, Shen L, Zhang Q, Guo LB, Qian Q (2019). DNA damage and reactive oxygen species cause cell death in the rice local lesions 1 mutant under high light and high temperature. New Phytol 222, 349-365.
[33]	Rabbani MA, Maruyama K, Abe H, Khadri MA, Katsura K, Ito Y, Yoshiwara K, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003). Monitoring expression profiles of rice genes under cold, drought, and high-salinity stresses and abscisic acid application using cDNA microarray and RNA gel-blot analyses. Plant Physiol 133, 1755-1767.
[34]	Rezaul IM, Feng BH, Chen TT, Fu WM, Zhang CX, Tao LX, Fu GF (2019). Abscisic acid prevents pollen abortion under high-temperature stress by mediating sugar metabolism in rice spikelets. Physiol Plant 165, 644-663.
[35]	Sato H, Suzuki Y, Sakai M, Imbe T (2002). Molecular characterization of Wx-mq, a novel mutant gene for low- amylose content in endosperm of rice (Oryza sativa L.). Breed Sci 52, 131-135.
[36]	Shi WJ, Yin XY, Struik PC, Solis C, Xie FM, Schmidt RC, Huang M, Zou YB, Ye CR, Jagadish SVK (2017). High day- and night-time temperatures affect grain growth dynamics in contrasting rice genotypes. J Exp Bot 68, 5233-5245.
[37]	Suriyasak C, Harano K, Tanamachi K, Matsuo K, Tamada A, Iwaya-Inoue M, Ishibashi Y (2017). Reactive oxygen species induced by heat stress during grain filling of rice (Oryza sativa L.) are involved in occurrence of grain chalkiness. J Plant Physiol 216, 52-57.
[38]	Tang RS, Zheng JC, Jin ZQ, Zhang DD, Huang YH (2008). Possible correlation between high temperature-induced floret sterility and endogenous levels of IAA, GAs and ABA in rice (Oryza sativa L.). Plant Growth Regul 54, 37-43.
[39]	Vishwakarma K, Upadhyay N, Kumar N, Yadav G, Singh J, Mishra RK, Kumar V, Verma R, Upadhyay RG, Pandey M, Sharma S (2017). Abscisic acid signaling and abiotic stress tolerance in plants: a review on current knowledge and future prospects. Front Plant Sci 8, 161.
[40]	Wang YL, Wang L, Zhou JX, Hu SB, Chen HZ, Xiang J, Zhang YK, Zeng YJ, Shi QH, Zhu DF, Zhang YP (2019). Research progress on heat stress of rice at flowering stage. Rice Sci 26, 1-10.
[41]	Wei LX, Lv BS, Li XW, Wang MM, Ma HY, Yang HY, Yang RF, Piao ZZ, Wang ZH, Lou JH, Jiang CJ, Liang ZW (2017a). Priming of rice (Oryza sativa L.) seedlings with abscisic acid enhances seedling survival, plant growth, and grain yield in saline-alkaline paddy fields. Field Crop Res 203, 86-93.
[42]	Wei LX, Lv BS, Wang MM, Ma HY, Yang HY, Liu XL, Jiang CJ, Liang ZW (2015). Priming effect of abscisic acid on alkaline stress tolerance in rice (Oryza sativa L.) seedlings. Plant Physiol Biochem 90, 50-57.
[43]	Wei XJ, Jiao GA, Lin HY, Sheng ZH, Shao GN, Xie LH, Tang SQ, Xu QG, Hu PS (2017b). GRAIN INCOMPLETE FILLING 2 regulates grain filling and starch synthesis during rice caryopsis development. J Integr Plant Biol 59, 134-153.
[44]	Xu YF, Chu CC, Yao SG (2021). The impact of high-temperature stress on rice: challenges and solutions. Crop J 9, 963-976.
[45]	Xu YY, Ramanathan V, Victor DG (2018). Global warming will happen faster than we think. Nature 564, 30-32.
[46]	Ye NH, Jia LG, Zhang JH (2012). ABA signal in rice under stress conditions. Rice 5, 1.
[47]	Zhang CX, Feng BH, Chen TT, Fu WM, Li HB, Li GY, Jin QY, Tao LX, Fu GF (2018). Heat stress-reduced kernel weight in rice at anthesis is associated with impaired source-sink relationship and sugars allocation. Environ Exp Bot 155, 718-733.
[48]	Zhang CX, Fu GF, Yang XQ, Yang YJ, Zhao X, Chen TT, Zhang XF, Jin QY, Tao LX (2016). Heat stress effects are stronger on spikelets than on flag leaves in rice due to differences in dissipation capacity. J Agron Crop Sci 202, 394-408.
[49]	Zhao C, Liu B, Piao SL, Wang XH, Lobell DB, Huang Y, Huang MT, Yao YT, Bassu S, Ciais P, Durand JL, Elliott J, Ewert F, Janssens IA, Li T, Lin ED, Liu Q, Martre P, Müller C, Peng SS, Peñuelas J, Ruane AC, Wallach D, Wang T, Wu DH, Liu Z, Zhu Y, Zhu ZC, Asseng S (2017). Temperature increase reduces global yields of major crops in four independent estimates. Proc Natl Acad Sci USA 114, 9326-9331.
[50]	Zhao Q, Zhou LJ, Liu JC, Du XX, Asad MAU, Huang FD, Pan G, Cheng FM (2018). Relationship of ROS accumulation and superoxide dismutase isozymes in developing anther with floret fertility of rice under heat stress. Plant Physiol Biochem 122, 90-101.
[51]	Zong WB, Ren D, Huang MH, Sun KL, Feng JL, Zhao J, Xiao DD, Xie WH, Liu SQ, Zhang H, Qiu R, Tang WJ, Yang RQ, Chen HY, Xie XR, Chen LT, Liu YG, Guo JX (2021). Strong photoperiod sensitivity is controlled by cooperation and competition among Hd1, Ghd7 and DTH8 in rice heading. New Phytol 229, 1635-1649.

Primer name	Primer sequence (5'-3')
SalTF	CGAAATAATGTTCCATGGTGTT
SalTR	TGTACTACGGATCGGTGCAA
OsWsi18F	TGTGACTCGATCCAGCGTAG
OsWsi18R	GTTCCTGCTGAGAAGCCATC
OsACT1F	TTCCAGCCTTCCTTCATA
OsACT1R	AACGATGTTGCCATATAGAT
OsCATBF	GCTGGTGAGAGATACCGGTCA
OsCATBR	TCAACCCACCGCTGGAGA
OsAPX6F	CCCCAAGATCCCCATGATCTA
OsAPX6R	CCTCTGGCGGGCATTG
OsFe-SODF	CGACGCCGAGGAATTTCTAG
OsFe-SODR	AGGTGGTGTAAGTGTCTCTCATGC
OsCu/Zn-SODF	TGTGACGGGACTTACTCCTGG
OsCu/Zn-SODR	CACCCATTCGTAGTATCGCCA
Ghd7F	AATCCGGTACGCGTCCAGA
Ghd7R	CCAAGCTCAAGCCTACTAGG
WxF	TTGGGATACCAGCGTTGTGG
WxR	CGGTCTTTCCCCAAACCTTCT
OsAGPL2F	AAGCCGAGAGCGTGGTAAAA
OsAGPL2R	CAGGAGCGCTAGGGTTTTCA
OsAGPL3F	TGCCGATGAGCAACTGCATA
OsAGPL3R	TTATACGCCCGGGAAAGGTG

Primer name	Primer sequence (5'-3')
SalTF	CGAAATAATGTTCCATGGTGTT
SalTR	TGTACTACGGATCGGTGCAA
OsWsi18F	TGTGACTCGATCCAGCGTAG
OsWsi18R	GTTCCTGCTGAGAAGCCATC
OsACT1F	TTCCAGCCTTCCTTCATA
OsACT1R	AACGATGTTGCCATATAGAT
OsCATBF	GCTGGTGAGAGATACCGGTCA
OsCATBR	TCAACCCACCGCTGGAGA
OsAPX6F	CCCCAAGATCCCCATGATCTA
OsAPX6R	CCTCTGGCGGGCATTG
OsFe-SODF	CGACGCCGAGGAATTTCTAG
OsFe-SODR	AGGTGGTGTAAGTGTCTCTCATGC
OsCu/Zn-SODF	TGTGACGGGACTTACTCCTGG
OsCu/Zn-SODR	CACCCATTCGTAGTATCGCCA
Ghd7F	AATCCGGTACGCGTCCAGA
Ghd7R	CCAAGCTCAAGCCTACTAGG
WxF	TTGGGATACCAGCGTTGTGG
WxR	CGGTCTTTCCCCAAACCTTCT
OsAGPL2F	AAGCCGAGAGCGTGGTAAAA
OsAGPL2R	CAGGAGCGCTAGGGTTTTCA
OsAGPL3F	TGCCGATGAGCAACTGCATA
OsAGPL3R	TTATACGCCCGGGAAAGGTG

Treatments		Shoot length (cm)	Stem dry weight (g)	Panicle length (cm)	Panicle weight (g)
CK	-ABA	85.54±5.46 a	37.27±5.10 a	20.96±0.92 b	3.01±0.18 ab
	ABA1	86.70±5.38 a	38.19±3.92 a	21.41±0.94 ab	3.05±0.38 ab
	ABA2	86.54±3.47 a	39.90±4.39 a	21.64±0.90 ab	3.03±0.41 ab
	ABA3	87.88±3.74 a	41.52±2.66 a	21.79±0.61 a	3.12±0.42 a
	FLU	84.10±4.24 a	37.28±5.77 a	20.92±0.67 b	2.75±0.29 b
	PC	87.30±5.50 a	41.61±2.77 a	22.03±0.73 a	3.09±0.18 ab
HS	-ABA	82.64±3.23 a	34.79±4.08 a	19.96±0.86 b	2.42±0.29 b
	ABA1	85.01±6.15 a	36.08±3.69 a	20.42±0.66 ab	2.73±0.32 a
	ABA2	85.09±6.20 a	37.74±5.74 a	20.94±1.96 ab	2.86±0.21 a
	ABA3	87.02±3.37 a	38.55±5.34 a	20.85±0.44 ab	2.81±0.23 a
	FLU	83.00±4.00 a	36.96±3.34 a	19.84±0.73 b	2.36±0.19 b
	PC	85.03±3.98 a	37.56±2.99 a	21.28±1.03 a	2.89±0.40 a

Treatments		Shoot length (cm)	Stem dry weight (g)	Panicle length (cm)	Panicle weight (g)
CK	-ABA	85.54±5.46 a	37.27±5.10 a	20.96±0.92 b	3.01±0.18 ab
	ABA1	86.70±5.38 a	38.19±3.92 a	21.41±0.94 ab	3.05±0.38 ab
	ABA2	86.54±3.47 a	39.90±4.39 a	21.64±0.90 ab	3.03±0.41 ab
	ABA3	87.88±3.74 a	41.52±2.66 a	21.79±0.61 a	3.12±0.42 a
	FLU	84.10±4.24 a	37.28±5.77 a	20.92±0.67 b	2.75±0.29 b
	PC	87.30±5.50 a	41.61±2.77 a	22.03±0.73 a	3.09±0.18 ab
HS	-ABA	82.64±3.23 a	34.79±4.08 a	19.96±0.86 b	2.42±0.29 b
	ABA1	85.01±6.15 a	36.08±3.69 a	20.42±0.66 ab	2.73±0.32 a
	ABA2	85.09±6.20 a	37.74±5.74 a	20.94±1.96 ab	2.86±0.21 a
	ABA3	87.02±3.37 a	38.55±5.34 a	20.85±0.44 ab	2.81±0.23 a
	FLU	83.00±4.00 a	36.96±3.34 a	19.84±0.73 b	2.36±0.19 b
	PC	85.03±3.98 a	37.56±2.99 a	21.28±1.03 a	2.89±0.40 a

Treatments		Panicle number	Spikelets per panicle	Percentage of filled spikelets (%)	1000-kernel weight (g)	Harvest index (%)	Grain yield per hole (g)
CK	-ABA	14.22±1.86 a	124.73±14.91 a	84.89±2.11 a	22.97±1.21 a	49.41±1.79 a	34.31±4.49 a
	ABA1	14.44±1.74 a	121.55±12.92 a	85.21±1.76 a	22.80±1.45 a	48.77±1.99 a	33.90±4.47 a
	ABA2	14.78±1.64 a	124.81±13.85 a	84.63±2.78 a	22.43±1.06 a	48.39±1.72 a	34.73±3.50 a
	ABA3	15.33±1.32 a	128.67±13.16 a	84.00±2.63 a	21.85±0.91 a	48.53±1.23 a	35.92±1.81 a
	FLU	14.44±1.42 a	123.27±14.02 a	83.02±3.71 a	22.61±0.91 a	49.43±3.72 a	33.31±4.41 a
	PC	15.78±1.56 a	124.53±12.76 a	85.26±2.58 a	21.93±0.58 a	48.58±2.21 a	36.46±2.65 a
HS	-ABA	13.56±1.67 a	119.76±16.90 a	78.02±1.96 b	19.76±0.54 c	44.83±2.73 a	24.84±3.69 bc
	ABA1	14.22±1.39 a	119.04±11.58 a	81.93±1.63 a	20.72±0.81 abc	46.70±2.03 a	28.59±2.84 a
	ABA2	14.56±1.88 a	121.99±8.02 a	81.12±2.48 a	20.98±1.45 a	47.04±2.78 a	30.00±3.14 a
	ABA3	14.44±2.07 a	115.09±18.40 a	81.10±3.45 a	21.19±0.93 a	44.76±3.97 a	28.01±2.41 ab
	FLU	14.22±1.99 a	113.38±16.50 a	75.43±3.33 b	19.81±0.50 bc	42.69±2.39 a	23.74±2.29 c
	PC	14.78±1.30 a	116.31±14.12 a	81.89±4.24 a	20.79±1.32 ab	46.01±3.50 a	29.08±3.47 a

脱落酸对水稻抽穗开花期高温胁迫的诱抗效应

Priming Effect of Abscisic Acid on High Temperature Stress During Rice Heading-flowering Stage

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 51

相关文章 15

编辑推荐

Metrics

本文评价

Indexes	Treatment time (d)		Shoot length	Stem dry weight	Panicle length	Panicle weight	Panicle number	Spikelets per panicle	Percentage of filled spikelets	1000-kernel weight	Harvest index	Yield
Contents of O₂^{- .}	CK	0	0.059	0.135	0.173	0.063	0.161	-0.115	0.006	0.135	-0.023	0.128
		2	-0.028	-0.071	-0.081	-0.053	-0.143	0.134	-0.233	-0.138	-0.033	-0.151
		4	0.097	-0.02	-0.083	0.030	-0.176	0.196	-0.062	-0.181	-0.379*	-0.102
		6	0.209	0.150	0.152	0.257	0.040	0.053	0.019	-0.329*	-0.107	0.069
		8	-0.049	0.052	-0.066	0.061	-0.05	0.052	0.002	-0.104	-0.147	-0.072
	HS	0	-0.075	-0.348**	-0.359**	-0.117	-0.331*	0.076	0.004	0.055	-0.047	-0.203
		2	-0.099	-0.093	-0.463**	-0.510**	-0.138	-0.173	-0.448**	-0.421**	-0.413**	-0.600**
		4	-0.059	-0.195	-0.373**	-0.441**	-0.142	-0.029	-0.384**	-0.390**	-0.174	-0.435**
		6	-0.215	-0.249	-0.339*	-0.487**	-0.140	-0.070	-0.408**	-0.404**	-0.205	-0.450**
		8	0.101	-0.191	-0.257	-0.393**	-0.138	-0.106	-0.241	-0.330*	-0.275*	-0.375**
Contents of H₂O₂	CK	0	-0.181	-0.021	0.086	0.035	0.047	-0.163	0.222	-0.070	-0.109	-0.061
		2	0.070	0.024	0.134	0.171	0.030	0.061	-0.045	-0.066	0.028	0.048
		4	0.077	0.194	0.374*	0.353*	0.089	-0.046	-0.160	0.066	-0.188	0.018
		6	0.196	0.228	0.174	0.339*	0.070	0.034	0.068	-0.329*	-0.322*	0.050
		8	0.225	0.195	0.221	0.135	0.093	0.070	-0.107	-0.152	-0.123	0.065
	HS	0	0.097	0.168	0.130	0.290*	0.227	-0.251	0.151	0.166	-0.174	0.071
		2	-0.208	-0.210	-0.439**	-0.569**	-0.206	-0.033	-0.477**	-0.400**	-0.201	-0.502**
		4	-0.160	-0.051	-0.317*	-0.400**	-0.076	-0.063	-0.499**	-0.412**	-0.182	-0.435**
		6	-0.196	-0.252	-0.372**	-0.642**	-0.250	0.009	-0.493**	-0.505**	-0.169	-0.531**
		8	-0.120	-0.231	-0.346*	-0.510**	-0.121	-0.090	-0.500**	-0.533**	-0.212	-0.500**

[1]	周玉萍, 颜嘉豪, 田长恩. 保卫细胞中ABA信号调控机制研究进展[J]. 植物学报, 2022, 57(5): 684-696.
[2]	王雷, 种康. 鱼和熊掌的选择: 反向重复序列变异介导的玉米环境适应与产量平衡[J]. 植物学报, 2022, 57(5): 555-558.
[3]	魏和平, 芦涛, 贾绮玮, 邓飞, 朱浩, 岂泽华, 王玉玺, 叶涵斐, 殷文晶, 方媛, 穆丹, 饶玉春. 水稻抽穗期QTL定位及候选基因分析[J]. 植物学报, 2022, 57(5): 588-595.
[4]	胡海涛, 钱婷婷, 杨玲. 基于H2DCFDA荧光探针的植物活性氧检测方法[J]. 植物学报, 2022, 57(3): 320-326.
[5]	贾利霞, 齐艳华. 生长素代谢、运输及信号转导调控水稻粒型研究进展[J]. 植物学报, 2022, 57(3): 263-275.
[6]	杨凯如, 贾绮玮, 金佳怡, 叶涵斐, 王盛, 陈芊羽, 管易安, 潘晨阳, 辛德东, 方媛, 王跃星, 饶玉春. 水稻黄绿叶调控基因YGL18的克隆与功能解析[J]. 植物学报, 2022, 57(3): 276-287.
[7]	叶涵斐, 殷文晶, 管易安, 杨凯如, 陈芊羽, 俞淑颖, 朱旭东, 辛德东, 章薇, 王跃星, 饶玉春. 水稻籽粒维生素E QTL挖掘及候选基因分析[J]. 植物学报, 2022, 57(2): 157-170.
[8]	王璐瑶, 陈謇, 赵守清, 闫慧莉, 许文秀, 刘若溪, 麻密, 虞轶俊, 何振艳. 水稻镉积累特性的生理和分子机制研究概述[J]. 植物学报, 2022, 57(2): 236-249.
[9]	熊淑萍, 曹文博, 曹锐, 张志勇, 付新露, 徐赛俊, 潘虎强, 王小纯, 马新明. 水平结构配置对冬小麦冠层垂直结构、微环境及产量的影响[J]. 植物生态学报, 2022, 46(2): 188-196.
[10]	余泓, 李家洋. 是金子无论在何处都发光: 玉米和水稻驯化中的趋同选择[J]. 植物学报, 2022, 57(2): 153-156.
[11]	王霞, 严维, 周志勤, 常振仪, 郑敏婷, 唐晓艳, 吴建新. 水稻雄性不育突变体ms102的鉴定和基因定位[J]. 植物学报, 2022, 57(1): 42-55.
[12]	王田幸子, 朱峥, 陈悦, 刘玉晴, 燕高伟, 徐珊, 张彤, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻OsWRKY42是Xa21介导的抗白叶枯病途径新元件[J]. 植物学报, 2021, 56(6): 687-698.
[13]	孙浩哲, 王襄平, 张树斌, 吴鹏, 杨蕾. 阔叶红松林不同演替阶段凋落物产量及其稳定性的影响因素[J]. 植物生态学报, 2021, 45(6): 594-605.
[14]	尚江源, 淳雁, 李学勇. 水稻穗长基因PAL3的克隆及自然变异分析[J]. 植物学报, 2021, 56(5): 520-532.
[15]	周俭民. 免疫信号轴揭示水稻与病原菌斗争的秘密[J]. 植物学报, 2021, 56(5): 513-515.