植物学报 ›› 2021, Vol. 56 ›› Issue (5): 544-558.DOI: 10.11983/CBB21014

• 研究报告 • 上一篇    下一篇

小桐子半胱氨酸蛋白酶家族和相应miRNAs的鉴定及其对低温锻炼的响应

吴丹丹1, 陈永坤1,2, 杨宇1, 孔春艳1, 龚明1,*()   

  1. 1云南师范大学生命科学学院, 生物能源持续开发利用教育部工程研究中心, 云南省生物质能与环境生物技术重点实验室, 昆明 650500
    2新疆师范大学生命科学学院, 乌鲁木齐 830054
  • 收稿日期:2021-01-18 接受日期:2021-05-07 出版日期:2021-09-01 发布日期:2021-08-31
  • 通讯作者: 龚明
  • 作者简介:* E-mail: gongming6307@163.com
  • 基金资助:
    国家自然科学基金(31860062);国家自然科学基金(31460059)

Identification of the Cysteine Protease Family and Corresponding miRNAs in Jatropha curcas and Their Response to Chill-hardening

Dandan Wu1, Yongkun Chen1,2, Yu Yang1, Chunyan Kong1, Ming Gong1,*()   

  1. 1Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming 650500, China
    2School of Life Sciences, Xinjiang Normal University, Urumqi 830054, China
  • Received:2021-01-18 Accepted:2021-05-07 Online:2021-09-01 Published:2021-08-31
  • Contact: Ming Gong

摘要: 小桐子(Jatropha curcas)是一种极具潜力的能源植物及冷敏植物, 12°C低温锻炼可显著提高其耐冷性。在全基因组水平上对小桐子半胱氨酸蛋白酶家族及靶向其基因的miRNAs进行鉴定、生物信息学分析和表达特性分析, 并对该基因家族成员与miRNAs互作参与调控小桐子对低温锻炼的响应进行解析。结果表明, 在小桐子基因组中共鉴定到39个半胱氨酸蛋白酶基因, 定位于11条染色体上, 可分为6个亚家族(C1A、C2、C12、C13、C14和C15), 编码181-2 158个氨基酸残基的多肽, 均具有Cys和His活性位点。基于miRNA组和降解组测序结果, 发现有283个miRNAs靶向调控小桐子半胱氨酸蛋白酶基因家族的14个成员。对靶向JcDEK1JcRD21BJcXBCP3L的miRNAs在12°C低温锻炼过程中的共表达分析表明, 这些miRNAs参与半胱氨酸蛋白酶基因表达的调控, 且这种调控可能与低温锻炼诱导的小桐子耐冷性增强有关。研究结果有助于深入理解小桐子半胱氨酸蛋白酶基因家族功能及其与相应miRNAs的互作, 以及通过互作调控小桐子对低温的响应。

关键词: 小桐子, 半胱氨酸蛋白酶, 基因家族, miRNAs, 低温响应

Abstract: Jatropha curcas is a kind of promising energy plants, but also a chilling-sensitive plant, which can be chill- hardening at 12°C with significant improvement of its chilling tolerance. In this study, the cysteine protease gene family of J. curcas and their corresponding miRNAs were identified at the genome-wide level. The results showed that a total of 39 cysteine protease genes were identified in J. curcas genome, which were located on 11 chromosomes and could be divided into six subfamilies (C1A, C2, C12, C13, C14 and C15); all encoding 181-2 158 amino acids with Cys and His active sites. Based on the sequencing results of miRNAome and degradome, 283 miRNAs were found to be targeted to 14 members of cysteine protease gene family. In addition, the co-expression analysis of those miRNAs targeting to JcDEK1, JcRD21B and JcXBCP3L during chill-hardening demonstrated significantly negative correlation during the chill-hardening at 12°C, suggesting that these miRNAs are involved in the regulation of the cysteine protease genes, and this regulation should be related to the enhancement of chilling tolerance induced by the chill-hardening. This study will be helpful for better understanding the function of cysteine protease gene family in J. curcas and the interaction of the family genes with their corresponding miRNAs, and how this interaction regulates the response of J. curcas to low temperature.

Key words: Jatropha curcas, cysteine protease, gene family, microRNAs, low temperature response