粗山羊草CCT家族基因序列分析及激素响应

doi:10.11983/CBB16054

植物学报 ›› 2017, Vol. 52 ›› Issue (2): 188-201.DOI: 10.11983/CBB16054

粗山羊草CCT家族基因序列分析及激素响应

郑军¹, 乔玲¹, 赵佳佳¹, 乔麟轶², 张世昌², 常建忠², 汤才国^3,,A;^*(), 杨三维^1,,A;^*()

¹山西省农业科学院小麦研究所, 临汾 041000
²山西省农业科学院作物科学研究所, 作物遗传与分子改良山西省重点实验室, 太原 030031
³中国科学院合肥物质科学研究院技术生物与农业工程研究所, 合肥 230031

收稿日期:2016-03-18 接受日期:2016-08-04 出版日期:2017-03-01 发布日期:2017-04-05
通讯作者: 汤才国,杨三维
作者简介:# 共同第一作者
基金资助:
国家自然科学基金青年基金(No.31601307)、山西省农业攻关(No.20150311001-5)、山西省自然科学基金(No.2016011001)和山西省农业科学院博士基金(No.YBSJJ1503)

Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii

Zheng Jun¹, Qiao Ling¹, Zhao Jiajia¹, Qiao Linyi², Zhang Shichang², Chang Jianzhong², Tang Caiguo^3,^*(), Yang Sanwei^1,^*()

¹Institute of Wheat Research, Shanxi Academy of Agricultural Sciences, Linfen 041000, China
²Shanxi Key Laboratory of Crop Genetics and Molecular Improvement, Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
³Hefei Institutes of Physical Science, Institute of Technical Biology & Agriculture Engineering, Chinese Academy of Sciences, Hefei 230031, China

Received:2016-03-18 Accepted:2016-08-04 Online:2017-03-01 Published:2017-04-05
Contact: Tang Caiguo,Yang Sanwei
About author:# Co-first authors

摘要/Abstract

摘要： 开花是植物生长发育的重要过程。CCT家族基因在植物中广泛存在, 参与植物花期的调控过程。该文从粗山羊草(Aegilops tauschii)全基因组中分离出26个CCT基因, 它们分布于7对染色体上, 按照排列顺序将其命名为AetCCT1-26。AetCCT蛋白分子量介于14.9 kDa (AetCCT3)-83.2 kDa (AetCCT12)之间, 其中有25个蛋白包含完整的CCT保守结构域。系统发育分析显示, 12对粗山羊草/乌拉尔图小麦(Triticum urartu) CCT蛋白和9对粗山羊草/水稻(Oryza sativa) CCT蛋白为直系同源蛋白。通过公共数据的数字表达分析表明, AetCCT具有组织特异性和组成型2种表达形式, 其中AetCCT3、AetCCT4、AetCCT7及AetCCT9等9个基因在大部分组织中都有表达, 而AetCCT15、AetCCT21和AetCCT25等基因分别在种子、叶和根等少数组织中特异表达。AetCCT家族可以响应不同外源激素, 施用激素24小时和72小时后各成员对激素响应整体表现一致, 但不同成员对于不同激素的响应存在差异, 表明该家族成员在功能和行使方式等方面具有一定的多样性, 可能参与不同生长发育过程。光照条件影响AetCCT的表达, 说明光照和春化作用是影响与调控该家族基因表达的重要因素。研究结果有助于探索小麦(T. aestivum)进化、驯化和演变的规律, 以及认识重要农艺性状的形成与互作网络。

关键词: 粗山羊草, CCT基因家族, 序列分析, 激素响应

Abstract: The control of flowering time is a crucial environmental adaptation in plants; numerous CCT domain genes control flowering in plants. Bioinformatics was used for a genome-wide research of CCT domain genes in Aegilops tauschii. In this study, 26 CCT domain genes were identified in A. tauschii, distributed on seven chromosomes of the A. tauschii genome. The predicted molecular weight of this family was spread over 14.9 to 83.2 kDa, and 25 proteins contain a complete CCT conserved structure domain. Twelve pairs of A. tauschii-Triticum urartu and 9 pairs of A. tauschii-rice CCT proteins were orthologous in the phylogenetic tree. Specific expression and constitutive expression were found in the CCT gene family; nine AetCCT genes were constitutively expressed in all organisms, including AetCCT3, AetCCT4, AetCCT7 and AetCCT9; AetCCT15, AetCCT21 and AetCCT25 were specifically expressed in leaf, seed and roots of A. tauschii, respectively. Moreover, the members responded to phytohormone treatments differently, which suggested a complex function and characteristic in metabolism of this family. Light conditions affect the expression of AetCCT, and this gene family is regulated by photoperiod and vernalization. The results of this paper not only provide useful information for wheat evolution studies, but also provide theory basis for comprehensive understanding of formation and interaction characteristics of important traits.

Key words: Aegilops tauschii, CCT gene family, sequence analysis, responses to phytohormones

郑军, 乔玲, 赵佳佳, 乔麟轶, 张世昌, 常建忠, 汤才国, 杨三维. 粗山羊草CCT家族基因序列分析及激素响应. 植物学报, 2017, 52(2): 188-201.

Zheng Jun, Qiao Ling, Zhao Jiajia, Qiao Linyi, Zhang Shichang, Chang Jianzhong, Tang Caiguo, Yang Sanwei. Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii. Chinese Bulletin of Botany, 2017, 52(2): 188-201.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB16054

https://www.chinbullbotany.com/CN/Y2017/V52/I2/188

图/表 10

图1 粗山羊草CCT家族基因的染色体定位和QTL定位左侧为遗传标尺, 黑色表示抽穗期QTL与AetCCT的共定位结果。

Figure 1 Chromosome mapping and QTL distribution of CCT genes in Aegilops tauschii genomeGenetic scale is indicated on the left side of chromosomes, the heading date QTLs are indicated on the right side of the chromosomes in black.

图2 粗山羊草AetCCT基因结构棒状结构代表外显子部分, 线状代表内含子部分。

Figure 2 Intron-exon structures of CCT genes in Aegilops tauschiiExons are represented by bars, introns are represented by connecting lines.

图3 粗山羊草CCT蛋白基序的分布

Figure 3 Motif distribution of AetCCT proteins in Aegilops tauschii

图4 粗山羊草CCT蛋白序列的基序

Figure 4 Motifs of CCT proteins in Aegilops tauschii

图5 CCT蛋白的系统发生乌拉尔图小麦和粗山羊草的CCT蛋白分别用三角形和圆形标注。

Figure 5 Phylogenesis of CCT proteinsCCT proteins of Triticum urartu and Aegilops tauschii were indicated by triangles and circles, respectively.

图6 粗山羊草CCT基因启动子区激素响应的顺式作用组件

Figure 6 Cis-elements in CCT genes promoters in response to phytohormones of Aegilops tauschii

表1 粗山羊草CCT家族基因

Table 1 CCT gene family in Aegilops tauschii

Gene	Length (bp)	CDS	Scaffold	Location	Mapped seq.	Chromosome
AetCCT1	2211	AEGTA18365	Scaffold4528	49937-52147	AT1D0317	1DL
AetCCT2	1836	AEGTA21580	Scaffold71509	42038-43873	AT1D0523	1DL
AetCCT3	1333	AEGTA27323	Scaffold97182	45576-47039	AT1D0969	1DL
AetCCT4	3096	AEGTA10532	Scaffold38896	10923-14018	AT2D1124	2DS
AetCCT5	2241	AEGTA13911	Scaffold30755	94901-97141	AT2D1581	2DS
AetCCT6	2157	AEGTA00203	Scaffold30755	129179-131557	AT2D1581	2DS
AetCCT7	2226	AEGTA31460	Scaffold77907	6990-9215	AT3D2607	3DS
AetCCT8	1134	AEGTA06337	Scaffold4745	59908-61041	AT3D3130	3DL
AetCCT9	2692	AEGTA28548	Scaffold28581	76532-79223	AT4D3632	4DS
AetCCT10	606	AEGTA01709	Scaffold50727	43106-43711	AT4D3683	4DS
AetCCT11	3208	AEGTA21446	Scaffold108	140572-143968	AT4D3728	4DL
AetCCT12	4521	AEGTA31746	Scaffold2864	19243-23723	AT4D3886	4DL
AetCCT13	2234	AEGTA18304	Scaffold98624	23875-26108	BE403305	4DL
AetCCT14	2878	AEGTA13770	Scaffold12030	253420-256287	AT4D4194	4DL
AetCCT15	603	AEGTA33063	Scaffold24714	5731-6333	AT5D4519	5DL
AetCCT16	1059	AEGTA03508	Scaffold185863	19051-20109	AT5D4590	5DL
AetCCT17	2404	AEGTA04421	Scaffold53469	30253-32656	AT5D5030	5DL
AetCCT18	738	AEGTA32221	Scaffold7166	12609-13346	AT5D5201	5DL
AetCCT19	3597	AEGTA05461	Scaffold106936	10967-14734	AT6D5284	6DS
AetCCT20	1188	AEGTA21198	Scaffold137524	2620-3965	AT6D5798	6DL
AetCCT21	2031	AEGTA15475	Scaffold71269	16605-18635	AT7D6397	7DS
AetCCT22	2781	AEGTA31079	Scaffold66553	48279-51059	AT7D6416	7DS
AetCCT23	3075	AEGTA08066	Scaffold6305	52570-55644	AT7D6716	7DS
AetCCT24	1521	AEGTA13638	Scaffold7100	34283-35803	AT7D7002	7DL
AetCCT25	742	AEGTA03492	Scaffold116052	16157-16929	AT7D7027	7DL
AetCCT26	2036	AEGTA22574	Scaffold16211	9228-11710	AT7D7167	7DL

图7 粗山羊草CCT家族基因在不同组织和生长发育阶段的表达

Figure 7 Expression profile of CCT genes in different tissues and developmental stages of Aegilops tauschii

图8 粗山羊草CCT家族基因在不同激素处理下的表达分析(A) 处理24小时; (B) 处理72小时

Figure 8 Hierarchical clustering of CCT domain genes under different phytohormones treatments in Aegilops tauschii(A) Phytohormones treatment for 24 h; (B) Phytohormones treatment for 72 h

图9 粗山羊草CCT家族基因在不同光照条件下的表达分析NV-LD: 未春化-长日照(16小时光照/8小时黑暗); NV-SD: 未春化-短日照(8小时光照/16小时黑暗); LD: 春化-长日照(16小时光照/8小时黑暗); SD: 春化-短日照(8小时光照/16小时黑暗)。

Figure 9 Expression of CCT domain genes under different light treatments in Aegilops tauschiiNV-LD: Long-day condition without vernalization (16 h light/8 h dark); NV-SD: Short-day condition without vernalization (8 h light/16 h dark); LD: Long-day condition after vernalization (16 h light/8 h dark); SD: Short-day condition after vernalization (8 h light/16 h dark).

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粗山羊草CCT家族基因序列分析及激素响应

Whole-genome Analysis of CCT Gene Family and Their Responses to Phytohormones in Aegilops tauschii

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