植物颗粒结合淀粉合酶GBSS基因家族的进化

doi:10.11983/CBB16041

摘要/Abstract

摘要： 为全面理解植物颗粒结合淀粉合酶(GBSS)基因在植物中的进化模式并重建其进化历史, 利用20种陆生植物和2种藻类植物的基因组数据, 通过生物信息学手段, 深入挖掘和分析植物类群基因组中GBSS基因家族的构成和基因特点, 推测其可能的扩增和丢失规律。结果共识别42条同源序列。系统发育和进化分析表明, GBSS基因起源古老, 可能在所有绿色植物的祖先中就已经出现, 之后在进化过程中不断发生谱系的特异扩张和拷贝丢失, 并最终通过功能分化的形式在植物类群中被固定。

关键词: 基因组, 系统发育, 基因复制, 拷贝丢失, 功能分化

Abstract: We analyzed the evolutionary pattern of the granule-bound starch-synthase genes (GBSS) in 20 land plants and 2 algae from whole-genome data with bioinformatics methods. A total of 42 genes were detected and their sequence structures were characterized. Phylogenetic analysis revealed that the GBSS gene family had an ancient origin and complex evolutionary history. It probably appeared in the early evolutionary stage of green plants, then mostly experienced lineage-specific expansion and copy loss during evolution, and finally was fixed in different plant taxa via functional divergence.

Key words: genome, phylogeny, gene duplication,, copy loss, functional, divergence

王倩, 孙文静, 包颖. 植物颗粒结合淀粉合酶GBSS基因家族的进化. 植物学报, 2017, 52(2): 179-187.

Wang Qian, Sun Wenjing, Bao Ying. Evolutionary Pattern of the GBSS Gene Family in Plants. Chinese Bulletin of Botany, 2017, 52(2): 179-187.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB16041

https://www.chinbullbotany.com/CN/Y2017/V52/I2/179

图/表 4

图1 基于22种植物GBSS基因氨基酸序列构建的最大似然系统发育树分支上的数值代表大于50%的最大似然分析的靴带支持率。种名后数字代表种内的不同基因位点, 对这些位点的细节描述见表1。

Figure 1 The maximum likelihood tree of the granule-bound starch synthase gene family based on amino acid sequences of 22 plant speciesNumbers above the branches indicate bootstrap values above 50%. Numbers following species names indicate different gene loci as listed in Table 1.

图2 基于4种植物淀粉合酶基因的氨基酸同源序列构建的最大似然树分支上的数值代表大于50%的最大似然分析的靴带支持率。箭头示2条香蕉基因实为SSII而非GBSS。

Figure 2 Maximum likelihood tree of 4 species based on amino acid homologous sequences of the starch synthase genesNumbers above the branches indicate bootstrap values above 50%. Two genes of Musa acuminata indicated by arrow should be SSII rather than GBSS.

图3 大豆4个GBSS同源基因位点在染色体片段的同线性排列(A) 位点GM10G31540所在染色体片段; (B) 位点GM20- G36040所在染色体片段; (C) 位点GM16G02110所在染色体片段; (D) 位点GM07G05580所在染色体片段。箭头代表不同的基因及其在基因组上的排列方向。同源基因采用相同的颜色。图中框格标注的为4个GBSS基因。

Figure 3 Synteny alignment of the chromosome regions with 4 GBSS homologous genes in Glycine max(A) The chromosome region includes locus GM10G31540; (B) The chromosome region includes locus GM20G36040; (C) The chromosome region includes locus GM16G02110; (D) The chromosome region includes locus GM07G05580. Arrows indicate the direction of genes’ transcription. Homologous genes are shown in same colors. Four GBSS genes are in the frame.

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