染色体展片法观察拟南芥雄性减数分裂过程中的染色体形态

doi:10.11983/CBB19127

植物学报 ›› 2019, Vol. 54 ›› Issue (5): 620-624.DOI: 10.11983/CBB19127 cstr: 32102.14.CBB19127

染色体展片法观察拟南芥雄性减数分裂过程中的染色体形态

徐婉约,王应祥()

复旦大学生命科学学院, 上海 200438

收稿日期:2019-07-10 接受日期:2019-07-31 出版日期:2019-09-01 发布日期:2020-03-10
通讯作者: 王应祥
基金资助:
国家自然科学基金(31570314);国家自然科学基金(No.31870293)

Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana

Wanyue Xu,Yingxiang Wang()

School of Life Sciences, Fudan University, Shanghai 200438, China

Received:2019-07-10 Accepted:2019-07-31 Online:2019-09-01 Published:2020-03-10
Contact: Yingxiang Wang

摘要/Abstract

摘要： 减数分裂指DNA复制1次, 细胞核分裂2次, 产生染色体数目减半的单倍体配子, 是真核生物有性生殖所必需的环节。拟南芥(Arabidopsis thaliana)是分子遗传学研究的传统模式生物。近年来, 随着显微镜技术的快速发展, 利用细胞学方法观察拟南芥减数分裂过程中的染色体形态和同源染色体互作事件, 将有助于深入认识减数分裂的分子遗传机制。该文详细描述了染色体展片法观察拟南芥雄性减数分裂细胞中的染色体形态。

关键词: 拟南芥, 减数分裂细胞, 染色体展片, 染色体原位杂交

Abstract: Meiosis halves the number of chromosomes to produce haploid gametes through nuclear division twice following single round of DNA replication, and is essential for eukaryotic sexual reproduction. Arabidopsis thaliana is a traditional model organism used for molecular genetic study. Recently, with breakthroughs in microscopic technologies for observing chromosomes, analyses of chromosome morphology during meiosis using cytological methods have made great advances to understand molecular and genetic mechanisms in regulation of meiosis. In this study, we described in detail the chromosome spread technique we developed, which is well used for observation of chromosome behaviors in Arabidopsis male meiocytes.

Key words: Arabidopsis thaliana, meiocytes, chromosome spread, FISH

徐婉约,王应祥. 染色体展片法观察拟南芥雄性减数分裂过程中的染色体形态. 植物学报, 2019, 54(5): 620-624.

Wanyue Xu,Yingxiang Wang. Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana. Chinese Bulletin of Botany, 2019, 54(5): 620-624.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB19127

https://www.chinbullbotany.com/CN/Y2019/V54/I5/620

图/表 2

图1 拟南芥Col-0生态型雄性减数分裂细胞的染色体形态(DAPI染色) (A) 细线期; (B) 偶线期; (C) 粗线期; (D) 双线期; (E) 终变期; (F) 中期I; (G) 后期I; (H) 末期I; (I) 前期II; (J) 中期II; (K) 后期II; (L) 四分体。Bars=5 μm

Figure 1 Chromosome morphology of the male meiocytes of Arabidopsis thaliana Col-0 ecotype (DAPI staining) (A) Leptotene; (B) Zygotene; (C) Pachytene; (D) Diplotene; (E) Diakinesis; (F) Metaphase I; (G) Anaphase I; (H) Telophase I; (I) Prophase II; (J) Metaphase II; (K) Anaphase II; (L) Tetrads. Bars=5 μm

图2 拟南芥Col-0生态型雄性减数分裂细胞的染色体形态及着丝粒信号(FISH) (A)-(L) 同图1。Bars=5 μm

Figure 2 Chromosome morphology and centromere signals of male meiocytes of the Arabidopsis thaliana Col-0 ecotype (FISH) (A)-(L) see Figure 1. Bars=5 μm

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染色体展片法观察拟南芥雄性减数分裂过程中的染色体形态

Chromosome Behaviors of Male Meiocytes by Chromosome Spread in Arabidopsis thaliana

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