植物学报 ›› 2022, Vol. 57 ›› Issue (4): 479-489.DOI: 10.11983/CBB22066
郭书亚1, 艾金祥1, 陈虹宇1, 邵烨瑶1, 汪妍1, 王倩1, 叶怡彤1, 张雅婷1, 丁哲晓1, 吴昊辰1, 吴玉环2,3, 张建新4, 饶米德1,*(), 刘鹏1,*()
收稿日期:
2022-04-08
修回日期:
2022-05-12
出版日期:
2022-07-01
发布日期:
2022-07-14
通讯作者:
饶米德,刘鹏
作者简介:
sky79@zjnu.cn基金资助:
Guo Shuya1, Ai Jinxiang1, Chen Hongyu1, Shao Yeyao1, Wang Yan1, Wang Qian1, Ye Yitong1, Zhang Yating1, Ding Zhexiao1, Wu Haochen1, Wu Yuhuan2,3, Zhang Jianxin4, Rao Mide1,*(), Liu Peng1,*()
Received:
2022-04-08
Revised:
2022-05-12
Online:
2022-07-01
Published:
2022-07-14
Contact:
Rao Mide,Liu Peng
摘要: 铝毒是限制酸性土壤中作物产量的主要因素之一。番茄(Solanum lycopersicum)是适合在酸性土壤中种植的主要经济作物, 不同品种番茄对铝胁迫的响应存在差异, 因此, 筛选苗期耐铝毒种质对番茄生产及研究具有重要意义。以10个番茄品种为材料, 采用室内土培盆栽, 设置1 000 µmol∙L-1 AlCl3·6H2O处理, 测定反映植物铝胁迫下生长状况的16个形态、生理生化及光合指标。通过主成分分析, 将铝胁迫下番茄幼苗的16个指标转化为5个独立的综合指标, 累积贡献率达90.779%。基于耐铝性综合评价值(A)的系统聚类分析, 将供试种质划分为5类, 第I类为高度耐铝品种Qianxi, 第V类为高度不耐铝品种Puluowangsi。经多元线性逐步回归分析得出番茄苗期耐铝评价方程: y=0.046+0.405X6+0.515X10-0.207X15+0.028X3 (R2=0.997), 从16个指标中提取出与A值显著相关(P<0.01)的4个指标: 丙二醛含量(X3)、净光合速率(X6)、叶面积(X10)和地下部干重(X15)。利用评价方程可判断不同番茄品种苗期的耐铝性, 使番茄耐铝性鉴定工作快速简便。
郭书亚, 艾金祥, 陈虹宇, 邵烨瑶, 汪妍, 王倩, 叶怡彤, 张雅婷, 丁哲晓, 吴昊辰, 吴玉环, 张建新, 饶米德, 刘鹏. 基于主成分-聚类-逐步回归分析构建番茄苗期耐铝性综合评价体系. 植物学报, 2022, 57(4): 479-489.
Guo Shuya, Ai Jinxiang, Chen Hongyu, Shao Yeyao, Wang Yan, Wang Qian, Ye Yitong, Zhang Yating, Ding Zhexiao, Wu Haochen, Wu Yuhuan, Zhang Jianxin, Rao Mide, Liu Peng. Establishment of a Comprehensive Evaluation System for Aluminum Tolerance in Tomato Seedlings Based on Principal Component Analysis-Clustering Analysis-Stepwise Regression Analysis. Chinese Bulletin of Botany, 2022, 57(4): 479-489.
No. | Cultivar name | Origin |
---|---|---|
1 | Jinpeng1 | Xian jinpeng seed |
2 | Zhefen202 | Zhejiang Academy of Agricultural Sciences |
3 | Jinpeng3 | Xian jinpeng seed |
4 | Zhongshu4 | Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences |
5 | Puluowangsi | Shouguang Xinxinran Horticulture Co., Ltd. |
6 | Qianxi | Known-You Seed (China) Co., Ltd. |
7 | Hongshengnv | Hejin Agricultural Science and Technology Hebei Co., Ltd. |
8 | Xianke8 | JingyanYinong (Beijing) Seed Technology Co., Ltd. |
9 | Hezuo903 | Shanghai Tomato Institute |
10 | Nongbofen3 | Shijiazhuang Nongboshi Science and Tech- nology Development Co., Ltd. |
表1 供试番茄种质来源
Table 1 Source of test Solanum lycopersicum germplasms
No. | Cultivar name | Origin |
---|---|---|
1 | Jinpeng1 | Xian jinpeng seed |
2 | Zhefen202 | Zhejiang Academy of Agricultural Sciences |
3 | Jinpeng3 | Xian jinpeng seed |
4 | Zhongshu4 | Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences |
5 | Puluowangsi | Shouguang Xinxinran Horticulture Co., Ltd. |
6 | Qianxi | Known-You Seed (China) Co., Ltd. |
7 | Hongshengnv | Hejin Agricultural Science and Technology Hebei Co., Ltd. |
8 | Xianke8 | JingyanYinong (Beijing) Seed Technology Co., Ltd. |
9 | Hezuo903 | Shanghai Tomato Institute |
10 | Nongbofen3 | Shijiazhuang Nongboshi Science and Tech- nology Development Co., Ltd. |
No. | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 1.09 | 0.54 | 1.01 | 0.86 | 0.35 | 0.60 | 0.84 | 0.35 | 0.88 | 0.55 | 0.82 | 0.22 | 0.59 | 0.95 | 0.67 | 0.70 |
2 | 1.03 | 0.65 | 1.61 | 1.18 | 0.57 | 0.61 | 0.99 | 0.54 | 0.74 | 0.28 | 0.85 | 0.17 | 0.41 | 0.73 | 0.27 | 0.24 |
3 | 1.07 | 0.54 | 0.87 | 1.51 | 0.49 | 0.57 | 0.96 | 0.45 | 0.77 | 0.49 | 0.79 | 0.14 | 0.65 | 0.85 | 0.21 | 0.41 |
4 | 1.02 | 0.59 | 1.23 | 1.49 | 0.29 | 0.43 | 0.90 | 0.29 | 0.90 | 0.64 | 0.61 | 0.38 | 0.69 | 1.15 | 0.29 | 0.43 |
5 | 1.04 | 0.44 | 1.19 | 0.91 | 0.14 | 0.13 | 0.97 | 0.10 | 0.56 | 0.22 | 0.74 | 0.24 | 0.53 | 0.61 | 0.33 | 0.15 |
6 | 1.08 | 0.59 | 1.17 | 1.37 | 0.54 | 1.18 | 0.52 | 0.54 | 1.01 | 0.69 | 0.98 | 0.40 | 1.09 | 0.84 | 0.94 | 0.80 |
7 | 1.03 | 0.42 | 0.60 | 1.11 | 0.22 | 0.17 | 1.05 | 0.21 | 0.86 | 0.44 | 0.81 | 0.33 | 0.56 | 0.70 | 0.40 | 0.29 |
8 | 1.06 | 0.24 | 2.60 | 1.37 | 0.24 | 0.20 | 1.09 | 0.24 | 0.87 | 0.30 | 0.78 | 0.27 | 0.54 | 0.66 | 0.42 | 0.51 |
9 | 0.98 | 0.36 | 1.33 | 0.53 | 0.33 | 0.24 | 1.28 | 0.46 | 0.72 | 0.23 | 0.69 | 0.32 | 0.20 | 0.90 | 0.26 | 0.33 |
10 | 1.02 | 0.51 | 1.18 | 1.27 | 0.72 | 0.68 | 1.01 | 0.67 | 0.66 | 0.52 | 0.76 | 0.17 | 0.48 | 0.84 | 0.38 | 0.40 |
Average | 1.04 | 0.49 | 1.28 | 1.16 | 0.39 | 0.48 | 0.96 | 0.38 | 0.80 | 0.44 | 0.78 | 0.26 | 0.57 | 0.82 | 0.42 | 0.43 |
Coefficient variation | 0.00 | 0.02 | 0.29 | 0.10 | 0.03 | 0.10 | 0.04 | 0.03 | 0.02 | 0.03 | 0.01 | 0.01 | 0.05 | 0.03 | 0.05 | 0.04 |
表2 铝胁迫下番茄幼苗各单项指标的耐铝系数
Table 2 Aluminum tolerance coefficient of each individual index in Solanum lycopersicum seedlings under aluminum stress
No. | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 1.09 | 0.54 | 1.01 | 0.86 | 0.35 | 0.60 | 0.84 | 0.35 | 0.88 | 0.55 | 0.82 | 0.22 | 0.59 | 0.95 | 0.67 | 0.70 |
2 | 1.03 | 0.65 | 1.61 | 1.18 | 0.57 | 0.61 | 0.99 | 0.54 | 0.74 | 0.28 | 0.85 | 0.17 | 0.41 | 0.73 | 0.27 | 0.24 |
3 | 1.07 | 0.54 | 0.87 | 1.51 | 0.49 | 0.57 | 0.96 | 0.45 | 0.77 | 0.49 | 0.79 | 0.14 | 0.65 | 0.85 | 0.21 | 0.41 |
4 | 1.02 | 0.59 | 1.23 | 1.49 | 0.29 | 0.43 | 0.90 | 0.29 | 0.90 | 0.64 | 0.61 | 0.38 | 0.69 | 1.15 | 0.29 | 0.43 |
5 | 1.04 | 0.44 | 1.19 | 0.91 | 0.14 | 0.13 | 0.97 | 0.10 | 0.56 | 0.22 | 0.74 | 0.24 | 0.53 | 0.61 | 0.33 | 0.15 |
6 | 1.08 | 0.59 | 1.17 | 1.37 | 0.54 | 1.18 | 0.52 | 0.54 | 1.01 | 0.69 | 0.98 | 0.40 | 1.09 | 0.84 | 0.94 | 0.80 |
7 | 1.03 | 0.42 | 0.60 | 1.11 | 0.22 | 0.17 | 1.05 | 0.21 | 0.86 | 0.44 | 0.81 | 0.33 | 0.56 | 0.70 | 0.40 | 0.29 |
8 | 1.06 | 0.24 | 2.60 | 1.37 | 0.24 | 0.20 | 1.09 | 0.24 | 0.87 | 0.30 | 0.78 | 0.27 | 0.54 | 0.66 | 0.42 | 0.51 |
9 | 0.98 | 0.36 | 1.33 | 0.53 | 0.33 | 0.24 | 1.28 | 0.46 | 0.72 | 0.23 | 0.69 | 0.32 | 0.20 | 0.90 | 0.26 | 0.33 |
10 | 1.02 | 0.51 | 1.18 | 1.27 | 0.72 | 0.68 | 1.01 | 0.67 | 0.66 | 0.52 | 0.76 | 0.17 | 0.48 | 0.84 | 0.38 | 0.40 |
Average | 1.04 | 0.49 | 1.28 | 1.16 | 0.39 | 0.48 | 0.96 | 0.38 | 0.80 | 0.44 | 0.78 | 0.26 | 0.57 | 0.82 | 0.42 | 0.43 |
Coefficient variation | 0.00 | 0.02 | 0.29 | 0.10 | 0.03 | 0.10 | 0.04 | 0.03 | 0.02 | 0.03 | 0.01 | 0.01 | 0.05 | 0.03 | 0.05 | 0.04 |
Index | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
X1 | 1.00 | |||||||||||||||
X2 | 0.17 | 1.00 | ||||||||||||||
X3 | 0.00 | -0.48 | 1.00 | |||||||||||||
X4 | 0.40 | 0.30 | 0.13 | 1.00 | ||||||||||||
X5 | 0.07 | 0.55 | -0.08 | 0.31 | 1.00 | |||||||||||
X6 | 0.46 | 0.67* | -0.17 | 0.38 | 0.75* | 1.00 | ||||||||||
X7 | -0.70* | -0.60 | 0.22 | -0.46 | -0.28 | -0.80** | 1.00 | |||||||||
X8 | -0.08 | 0.47 | -0.06 | 0.14 | 0.96** | 0.72* | -0.17 | 1.00 | ||||||||
X9 | 0.44 | 0.16 | 0.04 | 0.41 | 0.01 | 0.47 | -0.56 | 0.05 | 1.00 | |||||||
X10 | 0.44 | 0.54 | -0.39 | 0.56 | 0.37 | 0.70* | -0.75* | 0.31 | 0.69* | 1.00 | ||||||
X11 | 0.61 | 0.27 | -0.05 | 0.16 | 0.38 | 0.65* | -0.63* | 0.31 | 0.40 | 0.24 | 1.00 | |||||
X12 | -0.17 | -0.14 | -0.02 | -0.03 | -0.41 | 0.04 | -0.26 | -0.28 | 0.59 | 0.32 | -0.03 | 1.00 | ||||
X13 | 0.69* | 0.39 | -0.18 | 0.62 | 0.14 | 0.68* | -0.93** | 0.04 | 0.66* | 0.77** | 0.56 | 0.40 | 1.00 | |||
X14 | -0.10 | 0.44 | -0.27 | 0.14 | 0.18 | 0.31 | -0.21 | 0.27 | 0.41 | 0.65* | -0.40 | 0.32 | 0.18 | 1.00 | ||
X15 | 0.62 | 0.17 | -0.05 | 0.06 | 0.16 | 0.67* | -0.79** | 0.15 | 0.63* | 0.56 | 0.72* | 0.42 | 0.72* | 0.06 | 1.00 | |
X16 | 0.65* | 0.16 | 0.06 | 0.25 | 0.27 | 0.70* | -0.69* | 0.30 | 0.78** | 0.72* | 0.50 | 0.32 | 0.671* | 0.40 | 0.84** | 1.00 |
表3 铝胁迫下番茄幼苗各单项指标的相关系数矩阵
Table 3 Correlation coefficient matrix of each individual index of Solanum lycopersicum seedlings under aluminum stress
Index | X1 | X2 | X3 | X4 | X5 | X6 | X7 | X8 | X9 | X10 | X11 | X12 | X13 | X14 | X15 | X16 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
X1 | 1.00 | |||||||||||||||
X2 | 0.17 | 1.00 | ||||||||||||||
X3 | 0.00 | -0.48 | 1.00 | |||||||||||||
X4 | 0.40 | 0.30 | 0.13 | 1.00 | ||||||||||||
X5 | 0.07 | 0.55 | -0.08 | 0.31 | 1.00 | |||||||||||
X6 | 0.46 | 0.67* | -0.17 | 0.38 | 0.75* | 1.00 | ||||||||||
X7 | -0.70* | -0.60 | 0.22 | -0.46 | -0.28 | -0.80** | 1.00 | |||||||||
X8 | -0.08 | 0.47 | -0.06 | 0.14 | 0.96** | 0.72* | -0.17 | 1.00 | ||||||||
X9 | 0.44 | 0.16 | 0.04 | 0.41 | 0.01 | 0.47 | -0.56 | 0.05 | 1.00 | |||||||
X10 | 0.44 | 0.54 | -0.39 | 0.56 | 0.37 | 0.70* | -0.75* | 0.31 | 0.69* | 1.00 | ||||||
X11 | 0.61 | 0.27 | -0.05 | 0.16 | 0.38 | 0.65* | -0.63* | 0.31 | 0.40 | 0.24 | 1.00 | |||||
X12 | -0.17 | -0.14 | -0.02 | -0.03 | -0.41 | 0.04 | -0.26 | -0.28 | 0.59 | 0.32 | -0.03 | 1.00 | ||||
X13 | 0.69* | 0.39 | -0.18 | 0.62 | 0.14 | 0.68* | -0.93** | 0.04 | 0.66* | 0.77** | 0.56 | 0.40 | 1.00 | |||
X14 | -0.10 | 0.44 | -0.27 | 0.14 | 0.18 | 0.31 | -0.21 | 0.27 | 0.41 | 0.65* | -0.40 | 0.32 | 0.18 | 1.00 | ||
X15 | 0.62 | 0.17 | -0.05 | 0.06 | 0.16 | 0.67* | -0.79** | 0.15 | 0.63* | 0.56 | 0.72* | 0.42 | 0.72* | 0.06 | 1.00 | |
X16 | 0.65* | 0.16 | 0.06 | 0.25 | 0.27 | 0.70* | -0.69* | 0.30 | 0.78** | 0.72* | 0.50 | 0.32 | 0.671* | 0.40 | 0.84** | 1.00 |
Index | Principle factors | ||||
---|---|---|---|---|---|
CI1 | CI2 | CI3 | CI4 | CI5 | |
X1 | 0.659 | -0.262 | -0.463 | -0.281 | 0.157 |
X2 | 0.568 | 0.546 | 0.244 | -0.377 | -0.082 |
X3 | -0.198 | -0.223 | -0.392 | 0.607 | 0.516 |
X4 | 0.508 | 0.047 | 0.010 | -0.206 | 0.788 |
X5 | 0.470 | 0.827 | -0.129 | 0.237 | 0.062 |
X6 | 0.902 | 0.363 | -0.077 | 0.126 | -0.093 |
X7 | -0.923 | 0.111 | 0.077 | 0.245 | 0.047 |
X8 | 0.409 | 0.795 | -0.024 | 0.424 | -0.062 |
X9 | 0.724 | -0.438 | 0.206 | 0.252 | 0.115 |
X10 | 0.864 | 0.010 | 0.412 | -0.092 | 0.110 |
X11 | 0.648 | 0.004 | -0.647 | -0.008 | -0.274 |
X12 | 0.249 | -0.648 | 0.481 | 0.253 | -0.214 |
X13 | 0.881 | -0.295 | -0.025 | -0.242 | 0.119 |
X14 | 0.357 | 0.137 | 0.850 | 0.158 | 0.074 |
X15 | 0.801 | -0.336 | -0.223 | 0.183 | -0.356 |
X16 | 0.841 | -0.233 | -0.016 | 0.359 | -0.030 |
Characteristic root | 7.111 | 2.764 | 2.092 | 1.326 | 1.231 |
Contribution rate (%) | 44.442 | 17.276 | 13.077 | 8.288 | 7.695 |
Cumulative contribution (%) | 44.442 | 61.719 | 74.796 | 83.084 | 90.779 |
Factor weight | 0.490 | 0.190 | 0.144 | 0.091 | 0.085 |
表4 铝胁迫下番茄幼苗16个单项指标的主成分分析
Table 4 Principal component analysis of 16 individual index of Solanum lycopersicum seedlings under aluminum stress
Index | Principle factors | ||||
---|---|---|---|---|---|
CI1 | CI2 | CI3 | CI4 | CI5 | |
X1 | 0.659 | -0.262 | -0.463 | -0.281 | 0.157 |
X2 | 0.568 | 0.546 | 0.244 | -0.377 | -0.082 |
X3 | -0.198 | -0.223 | -0.392 | 0.607 | 0.516 |
X4 | 0.508 | 0.047 | 0.010 | -0.206 | 0.788 |
X5 | 0.470 | 0.827 | -0.129 | 0.237 | 0.062 |
X6 | 0.902 | 0.363 | -0.077 | 0.126 | -0.093 |
X7 | -0.923 | 0.111 | 0.077 | 0.245 | 0.047 |
X8 | 0.409 | 0.795 | -0.024 | 0.424 | -0.062 |
X9 | 0.724 | -0.438 | 0.206 | 0.252 | 0.115 |
X10 | 0.864 | 0.010 | 0.412 | -0.092 | 0.110 |
X11 | 0.648 | 0.004 | -0.647 | -0.008 | -0.274 |
X12 | 0.249 | -0.648 | 0.481 | 0.253 | -0.214 |
X13 | 0.881 | -0.295 | -0.025 | -0.242 | 0.119 |
X14 | 0.357 | 0.137 | 0.850 | 0.158 | 0.074 |
X15 | 0.801 | -0.336 | -0.223 | 0.183 | -0.356 |
X16 | 0.841 | -0.233 | -0.016 | 0.359 | -0.030 |
Characteristic root | 7.111 | 2.764 | 2.092 | 1.326 | 1.231 |
Contribution rate (%) | 44.442 | 17.276 | 13.077 | 8.288 | 7.695 |
Cumulative contribution (%) | 44.442 | 61.719 | 74.796 | 83.084 | 90.779 |
Factor weight | 0.490 | 0.190 | 0.144 | 0.091 | 0.085 |
Index | Eigen vector of measured indicators | ||||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |
X1 | 0.247 | -0.157 | -0.320 | -0.244 | 0.142 |
X2 | 0.213 | 0.328 | 0.169 | -0.327 | -0.074 |
X3 | -0.074 | -0.134 | -0.271 | 0.527 | 0.465 |
X4 | 0.190 | 0.028 | 0.007 | -0.179 | 0.710 |
X5 | 0.176 | 0.497 | -0.089 | 0.205 | 0.056 |
X6 | 0.338 | 0.219 | -0.053 | 0.109 | -0.084 |
X7 | -0.346 | 0.067 | 0.053 | 0.213 | 0.043 |
X8 | 0.153 | 0.478 | -0.016 | 0.368 | -0.055 |
X9 | 0.272 | -0.263 | 0.143 | 0.219 | 0.104 |
X10 | 0.324 | 0.006 | 0.285 | -0.080 | 0.099 |
X11 | 0.243 | 0.002 | -0.447 | -0.007 | -0.247 |
X12 | 0.093 | -0.390 | 0.332 | 0.220 | -0.193 |
X13 | 0.330 | -0.177 | -0.017 | -0.210 | 0.107 |
X14 | 0.134 | 0.082 | 0.587 | 0.137 | 0.066 |
X15 | 0.300 | -0.202 | -0.154 | 0.159 | -0.321 |
X16 | 0.315 | -0.140 | -0.011 | 0.312 | -0.027 |
表5 铝胁迫下番茄幼苗各综合指标的特征向量值
Table 5 Characteristic vector values of the comprehensive indexes of Solanum lycopersicum seedlings under aluminum stress
Index | Eigen vector of measured indicators | ||||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | |
X1 | 0.247 | -0.157 | -0.320 | -0.244 | 0.142 |
X2 | 0.213 | 0.328 | 0.169 | -0.327 | -0.074 |
X3 | -0.074 | -0.134 | -0.271 | 0.527 | 0.465 |
X4 | 0.190 | 0.028 | 0.007 | -0.179 | 0.710 |
X5 | 0.176 | 0.497 | -0.089 | 0.205 | 0.056 |
X6 | 0.338 | 0.219 | -0.053 | 0.109 | -0.084 |
X7 | -0.346 | 0.067 | 0.053 | 0.213 | 0.043 |
X8 | 0.153 | 0.478 | -0.016 | 0.368 | -0.055 |
X9 | 0.272 | -0.263 | 0.143 | 0.219 | 0.104 |
X10 | 0.324 | 0.006 | 0.285 | -0.080 | 0.099 |
X11 | 0.243 | 0.002 | -0.447 | -0.007 | -0.247 |
X12 | 0.093 | -0.390 | 0.332 | 0.220 | -0.193 |
X13 | 0.330 | -0.177 | -0.017 | -0.210 | 0.107 |
X14 | 0.134 | 0.082 | 0.587 | 0.137 | 0.066 |
X15 | 0.300 | -0.202 | -0.154 | 0.159 | -0.321 |
X16 | 0.315 | -0.140 | -0.011 | 0.312 | -0.027 |
No. | Comprehensive index value | Subordinate function value | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
CI1 | CI2 | CI3 | CI4 | CI5 | U(X1) | U(X2) | U(X3) | U(X4) | U(X5) | |
1 | 1.880 | -0.145 | -0.126 | 0.902 | 0.973 | 0.528 | 0.444 | 0.626 | 0.398 | 0.003 |
2 | 1.452 | 0.251 | -0.452 | 1.075 | 1.537 | 0.292 | 0.848 | 0.282 | 0.547 | 0.472 |
3 | 1.713 | 0.184 | -0.111 | 0.575 | 1.545 | 0.435 | 0.780 | 0.641 | 0.116 | 0.479 |
4 | 1.740 | -0.188 | 0.226 | 0.763 | 1.707 | 0.450 | 0.400 | 0.996 | 0.279 | 0.614 |
5 | 1.007 | -0.311 | -0.356 | 0.576 | 1.184 | 0.048 | 0.275 | 0.383 | 0.117 | 0.178 |
6 | 2.744 | -0.115 | -0.295 | 0.999 | 1.252 | 1.002 | 0.474 | 0.447 | 0.482 | 0.235 |
7 | 1.370 | -0.266 | -0.056 | 0.444 | 1.055 | 0.247 | 0.321 | 0.699 | 0.004 | 0.071 |
8 | 1.256 | -0.578 | -0.716 | 1.600 | 2.171 | 0.185 | 0.002 | 0.004 | 1.000 | 1.001 |
9 | 0.923 | -0.047 | -0.144 | 1.219 | 0.994 | 0.001 | 0.544 | 0.607 | 0.671 | 0.020 |
10 | 1.681 | 0.397 | -0.231 | 0.998 | 1.425 | 0.418 | 0.997 | 0.515 | 0.481 | 0.379 |
表6 铝胁迫下番茄幼苗的综合指标值及隶属函数值
Table 6 Comprehensive index values and subordinate function values of Solanum lycopersicum seedlings under aluminum stress
No. | Comprehensive index value | Subordinate function value | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
CI1 | CI2 | CI3 | CI4 | CI5 | U(X1) | U(X2) | U(X3) | U(X4) | U(X5) | |
1 | 1.880 | -0.145 | -0.126 | 0.902 | 0.973 | 0.528 | 0.444 | 0.626 | 0.398 | 0.003 |
2 | 1.452 | 0.251 | -0.452 | 1.075 | 1.537 | 0.292 | 0.848 | 0.282 | 0.547 | 0.472 |
3 | 1.713 | 0.184 | -0.111 | 0.575 | 1.545 | 0.435 | 0.780 | 0.641 | 0.116 | 0.479 |
4 | 1.740 | -0.188 | 0.226 | 0.763 | 1.707 | 0.450 | 0.400 | 0.996 | 0.279 | 0.614 |
5 | 1.007 | -0.311 | -0.356 | 0.576 | 1.184 | 0.048 | 0.275 | 0.383 | 0.117 | 0.178 |
6 | 2.744 | -0.115 | -0.295 | 0.999 | 1.252 | 1.002 | 0.474 | 0.447 | 0.482 | 0.235 |
7 | 1.370 | -0.266 | -0.056 | 0.444 | 1.055 | 0.247 | 0.321 | 0.699 | 0.004 | 0.071 |
8 | 1.256 | -0.578 | -0.716 | 1.600 | 2.171 | 0.185 | 0.002 | 0.004 | 1.000 | 1.001 |
9 | 0.923 | -0.047 | -0.144 | 1.219 | 0.994 | 0.001 | 0.544 | 0.607 | 0.671 | 0.020 |
10 | 1.681 | 0.397 | -0.231 | 0.998 | 1.425 | 0.418 | 0.997 | 0.515 | 0.481 | 0.379 |
No. | CAC value | Rank | A value | Rank | WAC value | Rank |
---|---|---|---|---|---|---|
1 | 0.688 | 5 | 0.470 | 5 | 0.687 | 5 |
2 | 0.680 | 6 | 0.435 | 6 | 0.673 | 6 |
3 | 0.673 | 7 | 0.505 | 4 | 0.668 | 7 |
4 | 0.707 | 3 | 0.517 | 3 | 0.701 | 2 |
5 | 0.518 | 10 | 0.157 | 10 | 0.509 | 10 |
6 | 0.859 | 1 | 0.709 | 1 | 0.859 | 1 |
7 | 0.576 | 8 | 0.289 | 7 | 0.569 | 8 |
8 | 0.712 | 2 | 0.268 | 8 | 0.694 | 4 |
9 | 0.573 | 9 | 0.255 | 9 | 0.565 | 9 |
10 | 0.704 | 4 | 0.545 | 2 | 0.700 | 3 |
Average | 0.669 | - | 0.415 | - | 0.662 | - |
Coefficient variation | 0.009 | - | 0.028 | - | 0.009 | - |
表7 供试番茄种质耐铝性评价的CAC值、A值和WAC值
Table 7 CAC value, A value and WAC value for evaluation of aluminum tolerance of test Solanum lycopersicum germplasms
No. | CAC value | Rank | A value | Rank | WAC value | Rank |
---|---|---|---|---|---|---|
1 | 0.688 | 5 | 0.470 | 5 | 0.687 | 5 |
2 | 0.680 | 6 | 0.435 | 6 | 0.673 | 6 |
3 | 0.673 | 7 | 0.505 | 4 | 0.668 | 7 |
4 | 0.707 | 3 | 0.517 | 3 | 0.701 | 2 |
5 | 0.518 | 10 | 0.157 | 10 | 0.509 | 10 |
6 | 0.859 | 1 | 0.709 | 1 | 0.859 | 1 |
7 | 0.576 | 8 | 0.289 | 7 | 0.569 | 8 |
8 | 0.712 | 2 | 0.268 | 8 | 0.694 | 4 |
9 | 0.573 | 9 | 0.255 | 9 | 0.565 | 9 |
10 | 0.704 | 4 | 0.545 | 2 | 0.700 | 3 |
Average | 0.669 | - | 0.415 | - | 0.662 | - |
Coefficient variation | 0.009 | - | 0.028 | - | 0.009 | - |
图1 供试番茄种质地上部表型 A-J表示编号1-10, 同表1。Bars=5 cm
Figure 1 The phenotype of test Solanum lycopersicum germplasms above ground A-J represent No.1-10, see Table 1. Bars=5 cm
Dependent variable | Multiple stepwise regression equation | r | R2 | F-value | P-value |
---|---|---|---|---|---|
A value | y=0.046+0.405X6+0.515X10-0.207X15+0.028X3 | 0.998 | 0.997 | 409.418 | 0.000 |
CAC value | y=0.295+0.173X6+0.0.083X3+0.226X10+0.108X9 | 0.997 | 0.993 | 178.487 | 0.000 |
WAC value | y=0.293+0.183X6+0.0.106X9+0.077X3+0.224X10 | 0.997 | 0.995 | 241.535 | 0.000 |
表8 基于回归分析的耐铝性模型预测
Table 8 Forecast of aluminum resistance model based on regression analysis
Dependent variable | Multiple stepwise regression equation | r | R2 | F-value | P-value |
---|---|---|---|---|---|
A value | y=0.046+0.405X6+0.515X10-0.207X15+0.028X3 | 0.998 | 0.997 | 409.418 | 0.000 |
CAC value | y=0.295+0.173X6+0.0.083X3+0.226X10+0.108X9 | 0.997 | 0.993 | 178.487 | 0.000 |
WAC value | y=0.293+0.183X6+0.0.106X9+0.077X3+0.224X10 | 0.997 | 0.995 | 241.535 | 0.000 |
No. | Primary value | Regression | Difference | Evaluation accuracy (%) |
---|---|---|---|---|
1 | 0.470 | 0.462 | 0.008 | 98.264 |
2 | 0.435 | 0.426 | 0.009 | 98.032 |
3 | 0.505 | 0.510 | -0.005 | 98.992 |
4 | 0.517 | 0.524 | -0.007 | 98.615 |
5 | 0.157 | 0.177 | -0.020 | 87.287 |
6 | 0.709 | 0.717 | -0.008 | 98.811 |
7 | 0.289 | 0.275 | 0.014 | 95.311 |
8 | 0.268 | 0.267 | 0.001 | 99.761 |
9 | 0.255 | 0.245 | 0.010 | 96.106 |
10 | 0.545 | 0.544 | 0.001 | 99.739 |
表9 10份番茄种质耐铝性评价回归方程的预测精度分析
Table 9 Analysis of forecast accuracy of the aluminum tolerance regression equation of 10 Solanum lycopersicum germplasms
No. | Primary value | Regression | Difference | Evaluation accuracy (%) |
---|---|---|---|---|
1 | 0.470 | 0.462 | 0.008 | 98.264 |
2 | 0.435 | 0.426 | 0.009 | 98.032 |
3 | 0.505 | 0.510 | -0.005 | 98.992 |
4 | 0.517 | 0.524 | -0.007 | 98.615 |
5 | 0.157 | 0.177 | -0.020 | 87.287 |
6 | 0.709 | 0.717 | -0.008 | 98.811 |
7 | 0.289 | 0.275 | 0.014 | 95.311 |
8 | 0.268 | 0.267 | 0.001 | 99.761 |
9 | 0.255 | 0.245 | 0.010 | 96.106 |
10 | 0.545 | 0.544 | 0.001 | 99.739 |
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