胡颓子属5种植物果实主要类胡萝卜素成分及含量

doi:10.11983/CBB15106

植物学报 ›› 2016, Vol. 51 ›› Issue (3): 306-310.DOI: 10.11983/CBB15106

胡颓子属5种植物果实主要类胡萝卜素成分及含量

胡海涛, 程珍霞, 李玲艳, 陈建华, 杨玲^*()

浙江师范大学化学与生命科学学院, 金华 321004

收稿日期:2015-06-15 接受日期:2015-07-24 出版日期:2016-05-01 发布日期:2016-05-24
通讯作者: 杨玲
作者简介:
? 共同第一作者
基金资助:
国家自然科学基金(No.31071775)、浙江科技计划(No.2008C24006)和浙江省重中之重学科开放基金(No.2011KFJJ001)

Determination of Carotenoids in Fruit of Five Elaeagnus spp. Genotypes by High Performance Liquid Chromatography

Haitao Hu, Zhenxia Cheng, Lingyan Li, Jianhua Chen, Ling Yang^*

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China

Received:2015-06-15 Accepted:2015-07-24 Online:2016-05-01 Published:2016-05-24
Contact: Yang Ling
About author:
? These authors contributed equally to this paper

摘要/Abstract

摘要： 利用高效液相色谱法对华东地区常见的胡颓子属(Elaeagnus) 4种生态型的胡颓子(E. pungens)、2个变种的大叶胡颓子(E. macrophylla)、蔓胡颓子(E. glabra)、银果胡颓子(E. magna)和佘山胡颓子(E. argyi) 9个样品成熟果实所含主要类胡萝卜素成分及含量进行了检测。结果表明, 5种胡颓子属植物成熟果实中的主要类胡萝卜素成分为番茄红素, 且在不同种间含量差异显著, 含量最高的是浙江嵊州的胡颓子, 其值为(259.89±26.22) µg·g^-1FW, 最低的是佘山胡颓子, 其含量为(91.19±7.74) µg·g^-1FW; 其中4种生态型的胡颓子的番茄红素含量都较高。研究表明这5种胡颓子属植物果实富含番茄红素, 是一类珍贵的天然番茄红素资源, 具有较大的市场开发应用价值。研究结果为揭示果实高积累番茄红素的机理提供了理想的研究材料。

Abstract: This study analyzed carotenoid types and content in the fruit of several Elaeagnus spp. including 4 ecotypes of E. pungens, 2 varieties of E. macrophylla, E. glabra, E. magna and E. argyi, by high performance liquid chromatography (HPLC). Lycopene is the major carotenoid in the fleshy pulp, and their content differed between the 5 genotypes. The greatest mean lycopene content was up to (259.89±26.22) µg·g^-1 fresh weight (FW) in E. pungens from Shengzhou of Zhejiang and the lowest was (91.19±7.74) µg·g^-1 FW in E. argyi. The lycopene content was high in all 4 ecotypes of E. pungens. The flesh of the 5 Elaeagnus spp. showed abundant lycopene, for beneficial fruit resources with important development and application value. These genotypes are also ideal research material for studying the mechanism of high accumulation of lycopene in fruit.

胡海涛, 程珍霞, 李玲艳, 陈建华, 杨玲. 胡颓子属5种植物果实主要类胡萝卜素成分及含量. 植物学报, 2016, 51(3): 306-310.

Haitao Hu, Zhenxia Cheng, Lingyan Li, Jianhua Chen, Ling Yang. Determination of Carotenoids in Fruit of Five Elaeagnus spp. Genotypes by High Performance Liquid Chromatography. Chinese Bulletin of Botany, 2016, 51(3): 306-310.

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https://www.chinbullbotany.com/CN/Y2016/V51/I3/306

图/表 4

图1 9个胡颓子属植物果实样品（(A) 胡颓子(安徽宣城); (B) 胡颓子(浙江嵊州); (C) 胡颓子(江西宜春); (D) 胡颓子(浙江金华); (E) 大叶胡颓子(酸); (F) 大叶胡颓子(甜); (G) 蔓胡颓子; (H) 银果胡颓子; (I) 佘山胡颓子。Bar=1 cm）

Fig. 1 Samples of nine Elaeagnus spp. fruits （(A) E. pungens (Xuancheng, Anhui); (B) E. pungens (Sheng- zhou, Zhejiang); (C) E. pungens (Yichun, Jiangxi); (D) E. pungens (Jinhua, Zhejiang); (E) E. macrophylla (acid); (F) E. macrophylla (sweet); (G) E. glabra; (H) E. magna; (I) E. argyi. Bar=1 cm）

表1 9个胡颓子属植物果实样品及其采样地点

Table 1 Nine ripe Elaeagnus spp. fruits and sampling site

Genotypes	Genotype	Sampling site
1	E. pungens	Xuancheng, Anhui
2	E. pungens	Shengzhou, Zhejiang
3	E. pungens	Yichun, Jiangxi
4	E. pungens	Jinhua, Zhejiang
5	E. macrophylla (acid)	Taizhou, Zhejiang
6	E. macrophylla (sweet)	Taizhou, Zhejiang
7	E. glabra	Jinhua, Zhejiang
8	E. magna	Yichun, Jiangxi
9	E. argyi	Jinhua, Zhejiang

图2 类胡萝卜素标准样品及9个胡颓子属植物果实样品的高效液相色谱图（(A) 类胡萝卜素标准样品; (B) 胡颓子(安徽宣城); (C) 胡颓子(浙江嵊州); (D) 胡颓子(江西宜春); (E) 胡颓子(浙江金华); (F) 大叶胡颓子(酸); (G) 大叶胡颓子(甜); (H) 蔓胡颓子; (I) 银果胡颓子; (J) 佘山胡颓子）

Fig. 2 The high performance liquid chromatograms of carotenoid standards and nine Elaeagnus spp. Fruits((A) Carotenoid standards; (B) E. pungens (Xuancheng, Anhui); (C) E. pungens (Shengzhou, Zhejiang); (D) E. pungens (Yichun, Jiangxi); (E) E. pungens (Jinhua, Zhejiang); (F) E. macrophylla (acid); (G): E. macrophylla (sweet); (H) E. glabra; (I) E. magna; (J) E. argyi )

表2 9个胡颓子属植物成熟果实样品番茄红素的含量(n=3)

Table 2 Lycopene content in nine ripe Elaeagnus spp. fruits (n=3)

Genotypes	Carotenoids	Carotenoid content (µg·g^-1 FW)
1	Lycopene	250.47±20.48
2	Lycopene	259.89±26.22
3	Lycopene	254.61±21.01
4	Lycopene	154.50±21.34
5	Lycopene	118.93±15.26
6	Lycopene	161.74±17.11
7	Lycopene	142.08±8.84
8	Lycopene	168.28±9.22
9	Lycopene	91.19±7.74

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胡颓子属5种植物果实主要类胡萝卜素成分及含量

Determination of Carotenoids in Fruit of Five Elaeagnus spp. Genotypes by High Performance Liquid Chromatography

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