长链非编码RNA基因Ef-cd调控水稻早熟与稳产

doi:10.11983/CBB19163

摘要/Abstract

摘要： 水稻(Oryza sativa)育种中早熟和高产往往不能兼顾。最近一项研究分离鉴定了1个调控水稻早熟的基因Ef-cd (Early flowering-completely dominant)。Ef-cd编码一段长链非编码RNA (lncRNA), 该lncRNA与OsSOC1基因的一段反义转录本重叠, Ef-cd正调控H3K36me3的富集及OsSOC1基因的表达。在不同的纬度, 含有Ef-cd基因的品种及其杂交种与对照相比生育期缩短7-20天, 但产量不降低。对1 439份优质杂交稻品种的基因型分析显示, 含有Ef-cd基因的16份纯合子和299份杂合子均表现出明显的早熟特性。Ef-cd基因可能通过提高氮素利用率及光合效率来促进水稻早熟。因此, Ef-cd基因兼顾了水稻的早熟和产量, 在水稻分子育种中具有重要利用价值。

关键词: 水稻, lncRNA, 抽穗期, 产量

Abstract: In rice breeding, ‘high yielding’ and ‘early maturing’ are negative-reciprocally regulated traits. A recent study identified a major maturity duration regulatory gene, Early flowering-completely dominant (Ef-cd), which encodes a long noncoding RNA. Ef-cd IncRNA overlaps with the antisense transcript of the OsSOC1 gene. Ef-cd positively regulates the expression of OsSOC1 and H3K36me3 deposition. Varieties and their derivatived hybrids harboring Ef-cd allele show 7-20 d early-maturation compared to their respective wild types at different latitudes, but without a concomitant yield penalty. Moreover, among 1 439 elite hybrid rice varieties, all of the hybrid cultivars with homozygous or heterozygous Ef-cd alleles mature significantly earlier. The mechanism of Ef-cd-promoted maturity without yield penalty is attributed to its facilitating nitrogen utilization and improving photosynthesis. Thus, Ef-cd may balance early maturating with stable grain yield, and can be used for molecular design breeding in rice.

Key words: rice, lncRNA, heading date, yield

张硕, 吴昌银. 长链非编码RNA基因Ef-cd调控水稻早熟与稳产. 植物学报, 2019, 54(5): 550-553.

Shuo Zhang, Changyin Wu. Long Noncoding RNA Ef-cd Promotes Maturity Without Yield Penalty in Rice. Chinese Bulletin of Botany, 2019, 54(5): 550-553.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB19163

https://www.chinbullbotany.com/CN/Y2019/V54/I5/550

图/表 1

图1 Ef-cd实现水稻早熟与稳产的工作模型 Ef-cd编码的lncRNA与OsSOC1基因的一段反义转录本重叠。 Ef-cd正调控OsSOC1基因的表达, 进而提高成花素基因Hd3a和RFT1的表达水平, 缩短水稻生育期。同时, Ef-cd通过提高氮素利用率及光合效率实现水稻的早熟与稳产。

Figure 1 A proposed working model showing Ef-cd-promoted maturity without yield penalty in rice Ef-cd (Early flowering-completely dominant) encodes a long noncoding RNA, which overlaps with the antisense transcript of the OsSOC1 gene. Ef-cd lncRNA positively regulates the expression of OsSOC1 and florigen genes Hd3a and RFT1. Ef-cd could promote rice flowering. On the other hand, Ef-cd could facilitate nitrogen utilization and improve photosynthesis. Thus, Ef-cd contributes to early maturating with stable grain yield.

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