一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应

doi:10.3724/SP.J.1259.2012.00474

植物学报 ›› 2012, Vol. 47 ›› Issue (5): 474-482.DOI: 10.3724/SP.J.1259.2012.00474 cstr: 32102.14.SP.J.1259.2012.00474

一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应

许凤莲, 李姣姣, 杜春晖, 王娜, 张素巧^*

河北师范大学生命科学学院, 河北省分子细胞生物学重点实验室, 石家庄 050016

收稿日期:2011-10-28 修回日期:2012-02-24 出版日期:2012-09-01 发布日期:2012-07-24
通讯作者: 张素巧
基金资助:
水稻类受体激酶OsWRL1调控雌配子体发育的机制研究及其同源基因的功能分析;水稻中WAK样受体激酶WRL1基因在生殖过程中的功能研究;水稻受体激酶WRL1基因在结实过程中的功能研究

OsSRL, a Receptor-like Kinase with S-locus Site, Is Involved in Drought Tolerance in Rice

Fenglian Xu, Jiaojiao Li, Chunhui Du, Na Wang, Suqiao Zhang^*

Key Laboratory of Molecular Cell Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang 050016, China

Received:2011-10-28 Revised:2012-02-24 Online:2012-09-01 Published:2012-07-24
Contact: Suqiao Zhang

摘要/Abstract

摘要： 植物在进化过程中针对干旱、高盐和高低温等逆境胁迫形成了多种适应机制, 植物类受体激酶作为重要的细胞信号传递分子在植物生长和抗逆境胁迫中发挥着重要功能。该文发现一个具有S位点的类受体激酶基因OsSRL可能参与水稻(Oryza sativa)的干旱胁迫反应。利用RNAi技术降低OsSRL的表达水平后, 转基因植株抗旱性增强, 并表现出幼苗存活率、叶绿素含量及鲜重增加等表型。进一步的研究表明30%PEG和100 μmol·L^–1ABA可诱导OsSRL基因表达, 利用RNAi降低其表达导致干旱诱导基因RAB16A及LEA3表达水平明显增加。表达模式分析发现OsSRL在胚芽、胚根、根、茎节以及花中表达。以上结果表明, OsSRL表达水平的降低增强植物的干旱耐受性, 其作为一个S-位点样类受体激酶可能参与了水稻对干旱胁迫的反应。

关键词: 干旱胁迫, 类受体激酶, 水稻, RNA干涉, S-位点

Abstract: Plants have developed various adaptive mechanisms to drought, salt, temperature and other stresses. Receptor-like kinases play significant roles in plant growth regulation and responses to stresses. We found that a receptor-like kinase with an S-locus domain, OsSRL, might be involved in drought stress response in rice. Downregulation of OsSRL expression by RNA interference produced enhanced drought tolerance and increased seedling survival rate, chlorophyll content and fresh weight as compared with control plants after drought stress treatment. The expression of OsSRL was induced by 30% PEG and 100 μmol·L^–1 abscisic acid. Knocking down OsSRL expression enhanced the expression of drought-response genes RAB16A and LEA3. OsSRL was expressed in plumule, radicula, root, node, and flower. These data suggest that OsSRL, as a receptor kinase with an S-locus domain, may play roles in drought stress tolerance in rice.

Key words: drought stress, receptor-like kinase, rice, RNA interference, S-locus

许凤莲, 李姣姣, 杜春晖, 王娜, 张素巧. 一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应. 植物学报, 2012, 47(5): 474-482.

Fenglian Xu, Jiaojiao Li, Chunhui Du, Na Wang, Suqiao Zhang. OsSRL, a Receptor-like Kinase with S-locus Site, Is Involved in Drought Tolerance in Rice. Chinese Bulletin of Botany, 2012, 47(5): 474-482.

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链接本文: https://www.chinbullbotany.com/CN/10.3724/SP.J.1259.2012.00474

https://www.chinbullbotany.com/CN/Y2012/V47/I5/474

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一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应

OsSRL, a Receptor-like Kinase with S-locus Site, Is Involved in Drought Tolerance in Rice

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