基于MADS-box诱饵与蛋白质相互作用的拟南芥花瓣发育分子网络拓展

doi:10.11983/CBB14169

植物学报 ›› 2015, Vol. 50 ›› Issue (5): 614-622.DOI: 10.11983/CBB14169

基于MADS-box诱饵与蛋白质相互作用的拟南芥花瓣发育分子网络拓展

杨黎^1,2, 孙丛苇^1,2, 代志军^1,2, 何淼^3*, 袁哲明^1,2*

¹湖南农业大学湖南省作物种质创新与资源利用重点实验室, 长沙 410128
²湖南农业大学湖南省植物病虫害生物学及防控重点实验室, 长沙 410128;
³中山大学生命科学学院, 广州 510275

收稿日期:2014-09-15 修回日期:2015-03-18 出版日期:2015-09-01 发布日期:2015-10-09
通讯作者: 何淼, 袁哲明
基金资助:
教育部博士点基金项目;国家自然科学基金重大研究计划培育项目;中央高校基本科研业务费专项资金项目

Expansion of the Molecular Network Related to Petal Development Based on MADS-box Proteins and Protein-Protein Interaction Network in Arabidopsis thaliana

Li Yang^1,2, Congwei Sun^1,2, Zhijun Dai^1,2, Miao He^3*, Zheming Yuan^1,2*

¹Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China;

²Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China;

³School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

Received:2014-09-15 Revised:2015-03-18 Online:2015-09-01 Published:2015-10-09
Contact: Miao He, Zheming Yuan

摘要/Abstract

摘要： 阐明花器官发育调控机理具重要的进化、发育和生态学意义。该文以拟南芥(Arabidopsis thaliana)花瓣发育为例, 整合蛋白质互作、亚细胞定位、基因芯片和基因功能注释等数据库, 通过组建蛋白质互作可信预测模型, 获得拟南芥花瓣蛋白质互作网络, 以含有MADS-box结构域蛋白为诱饵在网络中进行一级拓展, 得到含38个蛋白质和67对互作的拓展网络。基于拓展网络, DAVID基因功能注释表明, 多数蛋白质涉及的生物学过程与花发育调控相关; 提取到19个候选四元互作, 涉及ABCDE模型基因之外的8个基因, 其中含MADS-box结构域的AGL16可能是B类基因新成员或其冗余; SEU、LUH、CHR4、CHR11、CHR17和AT3G04960为拟南芥花瓣AP1-AP3-PI-SEP四聚体的候选靶标基因。研究结果为深入解析拟南芥花瓣发育分子调控网络奠定了基础。

Abstract: The regulatory mechanism of flower organ development has an important role in evolution, development and ecology. Here, we used petal development of Arabidopsis thaliana as an example and integrated protein-protein interaction, subcellular localization, gene-chip and gene functional annotation databases to reveal a protein-protein interaction network related to petals of A. thaliana and built a reliable predictive model of protein-protein interaction. By using proteins containing the MADS-box domain as bait, we could expand the network by one level and obtained an expanded network of 38 proteins and 67 protein-protein interactions. Gene functional annotation with the DAVID database suggested that most of the proteins were involved in regulation of flower development in the expanded network. We derived 19 candidate tetrameric interactions, involving 8 genes, from the expanded network. None of the 8 genes belonged to the ABCDE model genes: AGL16, with an MADS-box domain, may be a new member or a redundant gene of class B. SEU, LUH, CHR4, CHR11, CHR17, and AT3G04960 were candidate targets of petal AP1-AP3-PI-SEP tetramers of A. thaliana. The results provide references for deeply analyzing the molecular regulatory network related to petal development of A. thaliana.

中图分类号:

Q945.4

杨黎, 孙丛苇, 代志军, 何淼, 袁哲明. 基于MADS-box诱饵与蛋白质相互作用的拟南芥花瓣发育分子网络拓展. 植物学报, 2015, 50(5): 614-622.

Li Yang, Congwei Sun, Zhijun Dai, Miao He, Zheming Yuan. Expansion of the Molecular Network Related to Petal Development Based on MADS-box Proteins and Protein-Protein Interaction Network in Arabidopsis thaliana. Chinese Bulletin of Botany, 2015, 50(5): 614-622.

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链接本文: https://www.chinbullbotany.com/CN/10.11983/CBB14169

https://www.chinbullbotany.com/CN/Y2015/V50/I5/614

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基于MADS-box诱饵与蛋白质相互作用的拟南芥花瓣发育分子网络拓展

Expansion of the Molecular Network Related to Petal Development Based on MADS-box Proteins and Protein-Protein Interaction Network in Arabidopsis thaliana

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