Chin Bull Bot ›› 2019, Vol. 54 ›› Issue (1): 64-71.doi: 10.11983/CBB18010

• EXPERIMENTAL COMMUNICATIONS • Previous Articles     Next Articles

Effect of Conservation Conditions on Restricting Conservation of Acer rubrum cv. ‘Somerset’

Xian Yang,Dong Xin,Xie Xiaoman(),Wu Dan,Han Biao,Wang Yan   

  1. Shandong Forest Germplasm Resources Center, Jinan 250102, China
  • Received:2018-01-11 Accepted:2018-08-01 Online:2019-07-31 Published:2019-01-01
  • Contact: Xie Xiaoman E-mail:xxm529@126.com

Abstract:

We examined the effect of conservation conditions on restricting the conservation of Acer rubrum L. cv. ‘Somerset’, including photoperiod, light intensity, temperature and diurnal temperature variation. In the 182-day conservation period, the in vitro materials were mainly for differentiation in the early period and vegetative growth in the later period, also gradually adapted to the low-temperature environment. The effect of temperature on the growth of in vitro materials reached extremely significant level (P<0.01); the effect of photoperiod and light intensity lasted for a long time, and their interaction reached significant level (P<0.05). Diurnal temperature variation had a significant effect on average number of shoots and rate of rooting. In all of the tests, 25°C, illumination 12 h, 62.50 μmol·m -2·s -1 and 25°C, illumination 12 h, 31.25 μmol·m -2·s -1 conditions were both able to restrict conservation. The selection criteria for the best conservation conditions was not unique, and the core was keeping the differentiation ability of the in vitro materials.

Key words: restricting conservation, in vitro conservation, tissue culture, growth rhythm

Table 1

Experimental design of in vitro conservation of Acer rubrum L. cv. ‘Somerset’"

Treatment T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12
Photoperiod (h) 24 16 12 8 24 16 12 8 16 16 16 16
Light intensity (μmol·m-2·s-1) 62.50 62.50 62.50 62.50 31.25 31.25 31.25 31.25 62.50 62.50 62.50 31.25
Temperature (°C) 25 25 25 25 25 25 25 25 15 8 25/18* 25/18*

Figure 1

Growth curve of Acer rubrum cv. ‘Somerset’ (A) Differentiation rate of explants; (B) Average number of shoots; (C) Average height; (D) Rate of rooting. T1-T12 see Table 1."

Figure 2

In vitro conservation for 182 days of Acer rubrum cv. ‘Somerset’ (A) T1; (B) T2; (C) T3; (D) T4; (E) T5; (F) T6; (G) T7; (H) T8; (I) T9; (J) T10; (K) T11; (L) T12. T1-T12 see Table 1. Bars=1 cm"

Table 2

The influence of light and temperature on the in vitro conservation of Acer rubrum L. cv. ‘Somerset’"

Conservation days Significance testing of P and LI Significance testing
of Tm
Significance testing of DTV and LI
P LI P×LI Tm DTV LI DTV×LI
Differentiation rate of explants 7 0** 0.018* 0.002** 0** 0.05 0.84 0.589
14 0** 0.004** 0.036* 0.005** 0.914 0.009** 0.115
56 0.002** 0.075 0.001** 0** 0.771 0.283 0.302
182 0.297 0.907 0.01* 0** 0.322 0.86 0.564
Average number
of shoots
7 0** 0** 0** 0** 0** 0.695 0.248
14 0** 0.003** 0.002** 0.009** 0.013* 0.772 0.008**
28 0** 0.236 0** 0** 0.8 0.488 0.479
49 0.017* 0.248 0.033* 0** 0.003** 0.244 0.563
98 0.129 0.113 0.237 0.001** 0.036* 0.209 0.574
147 0.167 0.011* 0.193 0.006** 0.383 0.217 0.992
182 0.057 0.001** 0.008** 0.131 0.016* 0.03* 0.063
Average height 14 0.589 0.516 0.751 0.081 0.071 0.641 0.680
35 0.113 0.322 0.942 0** 0.845 0.077 0.744
91 0.021* 0** 0.004** 0.002** 0.464 0** 0.670
126 0.733 0.002** 0.039* 0.004** 0.636 0.016* 0.422
154 0.207 0.291 0.093 0.003** 0.646 0.549 0.501
182 0.187 0.294 0.182 0** 0.009** 0.034* 0.185
Rate of rooting 21 0.004** 0.041* 0.213 0.037* 0.541 0.025* 0.504
28 0** 0** 0.002** 0.001** 0.225 0.004** 0.658
35 0** 0** 0.012* 0** 0.409 0.008** 0.885
42 0.885 0.35 0** 0** 0.896 0.003** 0.336
49 0** 0.002** 0** 0** 0.018* 0.006** 0.415
56 0** 0.001** 0** 0** 0.005** 0.036* 0.905
63 0** 0.329 0.004** 0** 0.01* 0.49 0.432
70 0** 0.156 0.001** 0** 0.054 0.448 0.423
182 0.015* 0.284 0.007** 0** 0.09 0.713 0.901

Figure 3

Recovering culture for 28 days of Acer rubrum cv. ‘Somerset’ (A) Average number of shoots and average height; (B) Differentiation rate of explants and rate of rooting. T1-T12 see Table 1."

Figure 4

Recovering culture for 28 days of Acer rubrum cv. ‘Somerset’"

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