植物学报 ›› 2019, Vol. 54 ›› Issue (4): 464-473.DOI: 10.11983/CBB18188

• 研究报告 • 上一篇    下一篇

水稻耐储藏特性三年动态鉴定与QTL分析

刘进1,2,姚晓云1,2,余丽琴1,李慧1,周慧颖1,王嘉宇2,*(),黎毛毛1,*()   

  1. 1 江西省农业科学院水稻研究所/水稻国家工程实验室(南昌)/国家水稻改良中心南昌分中心, 南昌 330200
    2 沈阳农业大学水稻研究所, 沈阳 110866
  • 收稿日期:2018-09-03 接受日期:2019-02-11 出版日期:2019-07-10 发布日期:2020-01-08
  • 通讯作者: 王嘉宇,黎毛毛
  • 基金资助:
    国家重点研发计划(2016YFD0100101-11);江西现代农业科研协同创新专项(JXXTCX201701-01);江西省青年科学基金(20171BAB214026)

Detection and Analysis of Dynamic Quantitative Trait Loci at Three Years for Seed Storability in Rice (Oryza sativa)

Jin Liu1,2,Xiaoyun Yao1,2,Liqin Yu1,Hui Li1,Huiying Zhou1,Jiayu Wang2,*(),Maomao Li1,*()   

  1. 1 Nanchang Sub-center, National Rice Improvement Center/National Engineering Laboratory for Rice (Nanchang)/Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
    2 Rice Research Institute, Shenyang Agricultural University, Shenyang 110866, china
  • Received:2018-09-03 Accepted:2019-02-11 Online:2019-07-10 Published:2020-01-08
  • Contact: Jiayu Wang,Maomao Li

摘要: 种子耐储藏特性是粮食作物的特殊农艺性状之一, 耐储藏性能对种子生产和种质资源保存有重要意义。以粳型超级稻龙稻5 (LD5)和高产籼稻中优早8 (ZYZ8)杂交衍生的重组自交系(RILs)群体(共180个株系)为实验材料, 自然高温高湿条件下放置1年、2年和3年后, 对不同储藏时段种子发芽率进行比较, 并利用223个分子标记的遗传图谱进行动态QTL鉴定。结果表明, 不同储藏时段龙稻5的发芽率均显著低于中优早8, 株系间耐储性存在较大差异; 不同储藏时段发芽率显著相关, 相邻存储时段发芽率关系紧密。共检测到17个耐储性相关的QTLs, 3个老化时段分别检测到5、4和3个, 检测到5个动态条件QTLs, 单一QTL解释5.60%-32.76%的表型变异, 加性效应在-16.78%-16.95%范围内。主效QTL簇qSSC2qSSC6qSSC7qSSC8能调控不同储藏时段的发芽率, qSSC6具有明显降低发芽率的效应。共检测到26对上位性互作位点, 主效QTL qSS1qSS4参与上位性互作, 这表明上位性互作是调控耐储藏性状的重要遗传组成。研究结果为水稻(Oryza sativa)耐储性相关QTL的精细定位奠定基础, 同时丰富了耐储性分子标记辅助选择育种的基因资源。

关键词: 水稻, 自然老化, 种子耐储藏, QTL分析, 上位性互作

Abstract: Seed storability is one of the most important special agronomic traits of grain crops, which has great significance for seed production and germplasm conservation. In this study, we obtained a set of recombinant inbred lines (RILs), containing 180 lines and 223 molecular markers for a genetic map, derived from the cross between Longdao5 (LD5) and Zhongyouzao8 (ZYZ8). The set was used to identify quantitative trait loci (QTL) for seed storability traits under natural aging (higher temperature and humidity) at 1, 2 and 3 years after rice harvest. Then the germination rates of seeds at different storage stages were compared and identified by dynamic QTLs. The germination rate of the parents (the germination rate of LD5 was significantly lower than ZYZ8 under different storage stages) and RILs population was significant different under different storage stages, with a significant correlation of germination rate under different storage stages. A total of 17 QTLs were detected to control seed storability; 5, 4 and 3 QTLs were detected for 3 natural aging stages; and 5 dynamic conditional QTLs were detected; these QTLs explained 5.60% to 32.76% of the phenotypic variation, with an additive effect of -16.78% to 16.95%. Among these QTLs, 4 major clusters qSSC2, qSSC6, qSSC7 and qSSC8 were stably and reliably detected under 3 environments; the remaining QTLs were expressed in a single environment. qSSC6 reduced the germination rate. We detected 26 pairs of epistatic interaction sites. The major QTLs qSS1 and qSS4 were also involved in the epistasis effect, so epistatic interaction was an important genetic component for seed storability. These results will provide information for genetic analysis and related QTL fine mapping and will also enrich the gene resources for molecular marker-assisted selection of seed storability.

Key words: rice, natural aging, seed storability, QTL analysis, epistasis interaction