Chinese Bulletin of Botany ›› 2021, Vol. 56 ›› Issue (1): 25-32.DOI: 10.11983/CBB20148
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Chenyang Pan1, Hanfei Ye1, Weiyong Zhou3, Sheng Wang1, Mengjia Li1, Mei Lu1, Sanfeng Li2, Xudong Zhu2, Yuexing Wang2, Yuchun Rao1,*(), Gaoxing Dai3,*(
)
Received:
2020-08-26
Accepted:
2020-11-11
Online:
2021-01-01
Published:
2021-01-15
Contact:
Yuchun Rao,Gaoxing Dai
Chenyang Pan, Hanfei Ye, Weiyong Zhou, Sheng Wang, Mengjia Li, Mei Lu, Sanfeng Li, Xudong Zhu, Yuexing Wang, Yuchun Rao, Gaoxing Dai. QTL Mapping of Candidate Genes Involved in Cd Accumulation in Rice Grain[J]. Chinese Bulletin of Botany, 2021, 56(1): 25-32.
Primer name | Sequence (5'-3') | Tm (°C) | Length (bp) |
---|---|---|---|
LOC_Os02g50240-F-qrt | CTTCAAGGACCCATTCAGGA | 60.04 | 113 |
LOC_Os02g50240-R-qrt | TGAAGATCTTGGCAGCATTG | 59.95 | |
LOC_Os02g52780-F-qrt | GTTTTCAAAGGTGGGCTGAG | 59.71 | 148 |
LOC_Os02g52780-R-qrt | CCTCAGCTTCCAATTCCATC | 59.68 | |
LOC_Os09g31200-F-qrt | GCGACAAGAAGGATCAGGAG | 59.95 | 117 |
LOC_Os09g31200-R-qrt | AGAAGCAGTTCTGGCAGAGG | 59.75 | |
LOC_Os09g35030-F-qrt | GGATCAAGCAGGAGATGAGC | 59.92 | 135 |
LOC_Os09g35030-R-qrt | GCCTCGTCTCCCTGAACTT | 59.39 | |
LOC_Os09g37949-F-qrt | GGAAATCTGCCGATCAGTGT | 60.08 | 123 |
LOC_Os09g37949-R-qrt | GGGTCCTTCGATGGGTTTAT | 60.02 |
Table 1 Primer sequences for qRT-PCR
Primer name | Sequence (5'-3') | Tm (°C) | Length (bp) |
---|---|---|---|
LOC_Os02g50240-F-qrt | CTTCAAGGACCCATTCAGGA | 60.04 | 113 |
LOC_Os02g50240-R-qrt | TGAAGATCTTGGCAGCATTG | 59.95 | |
LOC_Os02g52780-F-qrt | GTTTTCAAAGGTGGGCTGAG | 59.71 | 148 |
LOC_Os02g52780-R-qrt | CCTCAGCTTCCAATTCCATC | 59.68 | |
LOC_Os09g31200-F-qrt | GCGACAAGAAGGATCAGGAG | 59.95 | 117 |
LOC_Os09g31200-R-qrt | AGAAGCAGTTCTGGCAGAGG | 59.75 | |
LOC_Os09g35030-F-qrt | GGATCAAGCAGGAGATGAGC | 59.92 | 135 |
LOC_Os09g35030-R-qrt | GCCTCGTCTCCCTGAACTT | 59.39 | |
LOC_Os09g37949-F-qrt | GGAAATCTGCCGATCAGTGT | 60.08 | 123 |
LOC_Os09g37949-R-qrt | GGGTCCTTCGATGGGTTTAT | 60.02 |
QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Limit of detection |
---|---|---|---|---|
qRCd-1 | 2 | 30302111-35902152 | 129.90-153.90 | 4.48 |
qRCd-2 | 3 | 5112031-5335542 | 21.91-22.87 | 2.51 |
qRCd-3 | 3 | 6338887-6654517 | 27.17-28.53 | 2.61 |
qRCd-4 | 9 | 13883217-15424344 | 59.51-66.12 | 4.32 |
qRCd-5 | 9 | 16779765-22230863 | 71.93-95.30 | 4.97 |
qRCd-6 | 9 | 22748818-22938953 | 97.52-98.33 | 2.60 |
qRCd-7 | 12 | 21976412-22336740 | 94.21-95.75 | 2.66 |
Table 2 QTL analysis of Cd contents in rice grains of the recombinant inbred line (RIL)
QTL | Chromosome | Physical distance (bp) | Position of support (cM) | Limit of detection |
---|---|---|---|---|
qRCd-1 | 2 | 30302111-35902152 | 129.90-153.90 | 4.48 |
qRCd-2 | 3 | 5112031-5335542 | 21.91-22.87 | 2.51 |
qRCd-3 | 3 | 6338887-6654517 | 27.17-28.53 | 2.61 |
qRCd-4 | 9 | 13883217-15424344 | 59.51-66.12 | 4.32 |
qRCd-5 | 9 | 16779765-22230863 | 71.93-95.30 | 4.97 |
qRCd-6 | 9 | 22748818-22938953 | 97.52-98.33 | 2.60 |
qRCd-7 | 12 | 21976412-22336740 | 94.21-95.75 | 2.66 |
Chromosome | Gene | Function | Object |
---|---|---|---|
2 | LOC_Os02g50240 | It participates in the regulation of various biological processes and stress response | Cytoplasmic glutamine synthetase |
2 | LOC_Os02g52780 | It regulates ABA-dependent stress-related gene expression to enhance the plant tolerance of drought and salt | Transcription factors bZIP |
9 | LOC_Os09g31200 | A major determinant of plant response to stress | Zinc finger protein |
9 | LOC_Os09g35030 | It specifically combines with drought response elements and controls many stress-induced genes | Transcription factors AP2/EREBP |
9 | LOC_Os09g37949 | It plays an important role in salt stress response | Receptor protein kinase |
Table 3 The function of candidate genes related to the accumulation of cadmium in rice
Chromosome | Gene | Function | Object |
---|---|---|---|
2 | LOC_Os02g50240 | It participates in the regulation of various biological processes and stress response | Cytoplasmic glutamine synthetase |
2 | LOC_Os02g52780 | It regulates ABA-dependent stress-related gene expression to enhance the plant tolerance of drought and salt | Transcription factors bZIP |
9 | LOC_Os09g31200 | A major determinant of plant response to stress | Zinc finger protein |
9 | LOC_Os09g35030 | It specifically combines with drought response elements and controls many stress-induced genes | Transcription factors AP2/EREBP |
9 | LOC_Os09g37949 | It plays an important role in salt stress response | Receptor protein kinase |
Figure 4 Differences in the expression level of candidate genes involved in cadmium accumulation in rice ** indicate extremely significant differences (P<0.01).
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