Chinese Bulletin of Botany ›› 2015, Vol. 50 ›› Issue (5): 614-622.DOI: 10.11983/CBB14169

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Expansion of the Molecular Network Related to Petal Development Based on MADS-box Proteins and Protein-Protein Interaction Network in Arabidopsis thaliana

Li Yang1,2, Congwei Sun1,2, Zhijun Dai1,2, Miao He3*, Zheming Yuan1,2*   

  1. 1Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China;

    2Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China;

    3School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2014-09-15 Revised:2015-03-18 Online:2015-09-01 Published:2015-10-09
  • Contact: Miao He, Zheming Yuan

Abstract:

The regulatory mechanism of flower organ development has an important role in evolution, development and ecology. Here, we used petal development of Arabidopsis thaliana as an example and integrated protein-protein interaction, subcellular localization, gene-chip and gene functional annotation databases to reveal a protein-protein interaction network related to petals of A. thaliana and built a reliable predictive model of protein-protein interaction. By using proteins containing the MADS-box domain as bait, we could expand the network by one level and obtained an expanded network of 38 proteins and 67 protein-protein interactions. Gene functional annotation with the DAVID database suggested that most of the proteins were involved in regulation of flower development in the expanded network. We derived 19 candidate tetrameric interactions, involving 8 genes, from the expanded network. None of the 8 genes belonged to the ABCDE model genes: AGL16, with an MADS-box domain, may be a new member or a redundant gene of class B. SEU, LUH, CHR4, CHR11, CHR17, and AT3G04960 were candidate targets of petal AP1-AP3-PI-SEP tetramers of A. thaliana. The results provide references for deeply analyzing the molecular regulatory network related to petal development of A. thaliana.

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