Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (5): 686-692.DOI: 10.11983/CBB17122

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Plant Regeneration by Inducing Axillary Buds of Sterile Seedlings of Primula denticulata

Zheng Yunfeng, Zhang Xiaoman*(), Liu Xiao   

  1. College of Landscape Architecture and Tourism, Hebei Agriculture University, Baoding 071000, China
  • Received:2017-06-28 Accepted:2017-10-07 Online:2018-09-01 Published:2018-11-29
  • Contact: Zhang Xiaoman
  • About author:

    † These authors contributed equally to this paper

Abstract: The axillary buds of sterile seedlings of Primula denticulata were used as explants. By disinfecting the explants, inducing adventitious buds, multiplication of adventitious buds, rooting culture and transplantation, the best medium of each stage was selected to establish the regeneration system of P. denticulata. The optimal medium of P. denticulata for inducing adventitious shoots from explants was MS+1 mg·L-1 NAA+0.1 mg·L-1 6-BA, and the induction rate was 73.33%. The optimal medium for inducing multiple shoots was MS+1 mg·L-1 NAA+0.5 mg·L-1 6-BA, and the proliferation rate was 85.19%. The optimal medium for rooting was 1/2MS+0.2 mg·L-1 NAA, and the rooting rate was 95.59%. Tissue cultured P. denticulata seedlings were transplanted into a mixture of peat and perlite, and the volume ratio was 3:1, with maximum survival rate 96.67%.

Key words: Primula denticulata, axillary bud of sterile seedling, multiple shoots, plant regeneration