Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata)

doi:10.11983/CBB18161

Abstract

Abstract: To improve regeneration efficiency of pumpkin (Cucurbita moschata), a high-efficiency regeneration system of plantlets was established. Cotyledons were used as explants and cultured on MS supplemented with 35 concentrations of phytohormone to induce shoots. The frequency of regeneration plantlets was affected by the concentration and proportion of phytohormone in medium. Within a certain concentration, 6-BA appeared to promote the efficiency of shoot regeneration. The addition of ABA in MS caused chlorosis of cotyledons; however, 6-BA and ABA combined could effectively promote the regeneration frequency of explants. Explants cultured on MS containing 1.0 mg∙L ^-1 6-BA and 0.5 mg∙L ^-1 ABA showed the highest frequency of shoot regeneration (90.26%). The elongated shoots were cultured on MS for rooting; plantlets appeared healthy after transplanting and nearly 70 days was required for explants to become a completely regenerated plant with the high-efficiency regeneration system for pumpkin.

Key words: cotyledons, phytohormone, plant regeneration, pumpkin

Jia Guo,Yansu Li,Chaoxing He,Yan Yan,Xianchang Yu. Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata)[J]. Chinese Bulletin of Botany, 2019, 54(4): 539-546.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18161

https://www.chinbullbotany.com/EN/Y2019/V54/I4/539

Figures/Tables 6

References 37

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Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	1.194^a	10	0.119	19.893	0.000
Intercept	11.738	1	11.738	1955.511	0.000
6-BA concentration	0.932	5	0.186	31.040	0.000
ABA concentration	0.291	5	0.058	9.699	0.000
Error	0.144	24	0.006
Total	13.664	35
Corrected total	1.338	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	1.194^a	10	0.119	19.893	0.000
Intercept	11.738	1	11.738	1955.511	0.000
6-BA concentration	0.932	5	0.186	31.040	0.000
ABA concentration	0.291	5	0.058	9.699	0.000
Error	0.144	24	0.006
Total	13.664	35
Corrected total	1.338	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	20.734^a	10	2.073	6.731	0.000
Intercept	116.473	1	116.473	378.098	0.000
6-BA concentration	11.488	5	2.298	7.458	0.000
ABA concentration	10.032	5	2.006	6.513	0.001
Error	7.393	24	0.308
Total	151.118	35
Corrected total	28.127	34

Source	Type III Sum of squares	df	Mean square	F	Sig.
Corrected model	20.734^a	10	2.073	6.731	0.000
Intercept	116.473	1	116.473	378.098	0.000
6-BA concentration	11.488	5	2.298	7.458	0.000
ABA concentration	10.032	5	2.006	6.513	0.001
Error	7.393	24	0.308
Total	151.118	35
Corrected total	28.127	34

Treatments	6-BA (mg∙L^-1)	Number of explants	Number of explants with adventitious shoots	Number of shoots	Average number of induced shoots	Rate of plant regeneration (%)
M1	0.5	60	39.78	73.60	1.85	66.31
M2	1.0	60	41.51	72.22	1.74	69.18
M3	1.5	60	34.01	50.00	1.47	56.69
M4	2.0	60	29.88	33.46	1.12	49.79
M5	2.5	60	27.16	31.50	1.16	45.26