Chinese Bulletin of Botany ›› 2019, Vol. 54 ›› Issue (4): 539-546.DOI: 10.11983/CBB18161

• TECHNIQUES AND METHODS • Previous Articles    

Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata)

Jia Guo,Yansu Li,Chaoxing He,Yan Yan,Xianchang Yu()   

  1. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2018-07-18 Accepted:2018-12-10 Online:2019-07-10 Published:2020-01-08
  • Contact: Xianchang Yu

Abstract: To improve regeneration efficiency of pumpkin (Cucurbita moschata), a high-efficiency regeneration system of plantlets was established. Cotyledons were used as explants and cultured on MS supplemented with 35 concentrations of phytohormone to induce shoots. The frequency of regeneration plantlets was affected by the concentration and proportion of phytohormone in medium. Within a certain concentration, 6-BA appeared to promote the efficiency of shoot regeneration. The addition of ABA in MS caused chlorosis of cotyledons; however, 6-BA and ABA combined could effectively promote the regeneration frequency of explants. Explants cultured on MS containing 1.0 mg∙L -1 6-BA and 0.5 mg∙L -1 ABA showed the highest frequency of shoot regeneration (90.26%). The elongated shoots were cultured on MS for rooting; plantlets appeared healthy after transplanting and nearly 70 days was required for explants to become a completely regenerated plant with the high-efficiency regeneration system for pumpkin.

Key words: cotyledons, phytohormone, plant regeneration, pumpkin