9-cis-epoxycarotenoid dioxygenase (NCED), a key rate-limiting enzyme in ABA biosynthesis in plants, is involved in plant drought, exogenous abscisic acid (ABA) and high salt response, and can reduce the damage of environmental stress on plants. With genome-wide identification and analysis of the grape NCED gene family, we aimed to understand the species evolution relationship and study the expression patterns of various genes in different tissues and under drought, ABA and high salt (NaCl) stress treatment, to lay the foundation for further study of the biological functions of NCED genes. A total of 12 NCED genes were found in the grape genome. The amino acid residues encoded by the genes are distributed between 510 aa (VvNCED2) and 625 aa (VvNCED10). The maximum molecular weight of the VvNCED protein was 70.53 kDa (VvNCED10) and the minimum was 57.85 kDa (VvNCED2). After differentiation from the ancestral gene, the grape NCED genes had five replication events with two loss events. The NCED1/2, NCED3/4, NCED6/7 and NCED9/10 gene pairs are thought to be produced by segmental duplication. The replication time of segmental duplication ranged from 3.08 to 120.0 million years ago, which is later than the differentiation of monocotyledons. As compared with the control, VvNCED1 was significantly upregulated by 72.1% after 48 h of ABA treatment, whereas VvNCED2 was significantly downregulated by 84.0%. The expression of VvNCED6 was higher in only roots under drought treatment for 14, 21 and 28 days than in the control: 2.49, 1.05 and 1.09 times of control values, respectively. The expression of VvNCED7 was only 1.07 times higher than the control value in roots under drought treatment for 14 days. After 72 h of ABA treatment, the expression of VvNCED3 was significantly downregulated by 59.5% as compared with the control, whereas VvNCED4 was significantly upregulated by 169.9% as compared with the control. The significant peaks in expression of VvNCED3/VvNCED4 after NaCl treatment were 24 and 48 h, respectively, up by 219.2% and 114.4%. The differential conserved-domain expression patterns with different stress treatments are the basis for the functional differentiation of NCED proteins. The functional differentiation of NCED during evolution may be conducive to the occurrence of replication events.