Regulation of Rice Growth by Root-secreted Phytohormones

doi:10.11983/CBB20133

Abstract

Abstract: The phytohormones are important small molecules synthesized in plants, and their contents change as the external and internal conditions vary. Small volume of growth media used in the hydroculture will restrict the growth of plants, which is generally thought to be caused by the shortage of nutrients. In this study, we found that rice seedlings grew faster in the large volume of water culture system than that in the small volume of water culture system. We measured the contents of different plant hormones in culture medium and rice seedlings using liquid chromatography-mass spectrum (LC-MS). It was found that there were more stress-response related phytohormones such as ABA, SA and JA-Ile accumulated in the plants growing in the small volume water system than that in the large volume water system, and finally resulted in a low growth rate and a biomass decline. Therefore, we speculate that plants can sense the water resource through actively sensing the concentration of root-secreted phytohormones, and adjust growth and development to better adapt to the environment. The study will help to understand the physiological functions of root-secreted phytohormones and optimize the hydroculture conditions in the area of plant factory.

Key words: culture volume, phytohormones, root exudate, growth and development, rice

Qilu Yu, Jiangzhe Zhao, Xiaoxian Zhu, Kewei Zhang. Regulation of Rice Growth by Root-secreted Phytohormones[J]. Chinese Bulletin of Botany, 2021, 56(2): 175-182.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB20133

https://www.chinbullbotany.com/EN/Y2021/V56/I2/175

Figures/Tables 4

Figure 1 The phenotype of 12-day rice seedlings grown in different water culture systems (A) Phenotypes of rice seedlings grown in different water culture systems for 12 days (bar=3 cm); (B) Plant height of rice seedlings grown in different water culture systems for 12 days (n=30); (C) Fresh weight of rice seedlings grown in different water culture systems for 12 days (n=30); (D) 0.1 and 4 L containers used in this experiment and the phenotypes of rice seedlings grown in them, respectively (bar=3 cm). ** significant difference at P<0.01; *** significant differences at P<0.001.

Figure 2 Hormone profiling of shoot of rice seedlings grown in different water culture sytems (A)-(J) The ABA, SA, JA-Ile, IAA, tZ, tZR, cZ, cZR, iP, and iPR contents in shoot of rice seedlings after planting in 0.1 and 4 L water culture systems for 12 days, respectively. * significant differences at P<0.05; *** significant differences at P<0.001.

Figure 3 Profiling of phytohormones in the root of rice after planting in different water culture systems (A)-(J)The ABA, SA, JA-Ile, IAA, tZ, tZR, cZ, cZR, iP, and iPR contents in the root of rice after planting in 0.1 and 4 L water culture systems, respectively. * significant differences at P<0.05; *** significant differences at P<0.001.

Figure 4 Phytohormone profiling of the different hydroculture systems (A)-(H) The ABA, SA, JA-Ile, IAA, cZ, cZR, iP, and iPR contents in 0.1 and 4 L water culture systems after being planted with rice seedlings for 12 days, respectively. * significant differences at P<0.05; *** significant differences at P<0.001.

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