Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (3): 334-340.DOI: 10.11983/CBB17207

Special Issue: 药用植物专辑

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Induction and Plant Regeneration of Protocorm-like Bodies in Dioscorea opposita

Li Ruixue1, Li Jiqiang1, Pu Tengfei1, Zhang Xiaoli1,2,3, Zhao Xiting1,2,3, Li Junhua1,2,3, Li Mingjun1,2,3,*()   

  1. 1College of Life Sciences, Henan Normal University, Xinxiang 453007, China
    2Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province, Xinxiang 453007, China
    3Henan Province Engineering Laboratory of Green Medicinal Plant Biotechnology, Xinxiang 453007, China
  • Received:2017-11-06 Accepted:2018-01-12 Online:2018-05-01 Published:2018-09-11
  • Contact: Li Mingjun

Abstract:

To improve the speed of in vitro propagation of Dioscorea opposita and shorten the breeding cycle, we used nodal segments with axillary buds of D. opposita cv. ‘Tiegun’ as the starting material. The optimal conditions for protocorm-like body (PLB) induction, proliferation, differentiation and plantlet regeneration were analyzed. The optimal culture medium for PLB induction was MS+1.0 mg·L-1 TDZ+30 g·L-1 sucrose, the optimal culture medium for PLB proliferation was MS+9 mg·L-1 6-BA+30 g·L-1 sucrose, and the optimal culture medium for PLB differentiation was MS+2 mg·L-1 KT+0.02 mg·L-1 NAA+30 g·L-1 sucrose. In culture medium containing 1/4MS+0.05 mg·L-1 NAA+1.0 mg·L-1 PP333+15 g·L-1 sucrose, the rooting rate of regenerated plantlets from PLBs was 80%, and the survival rate of regenerated plantlets was up to 85% after being sown in soil. The induction of protocorm like bodies and the establishment of plant regeneration system provide a new way for rapid propagation of D. opposita seedlings.

Key words: Dioscorea opposita, induction, plant regeneration, proliferation, protocorm-like body