Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (3): 334-340.DOI: 10.11983/CBB17207
Special Issue: 药用植物专辑 (2018年53卷3期)
• TECHNIQUES AND METHODS • Previous Articles Next Articles
Li Ruixue1, Li Jiqiang1, Pu Tengfei1, Zhang Xiaoli1,2,3, Zhao Xiting1,2,3, Li Junhua1,2,3, Li Mingjun1,2,3,*()
Received:
2017-11-06
Accepted:
2018-01-12
Online:
2018-05-01
Published:
2018-09-11
Contact:
Li Mingjun
Li Ruixue, Li Jiqiang, Pu Tengfei, Zhang Xiaoli, Zhao Xiting, Li Junhua, Li Mingjun. Induction and Plant Regeneration of Protocorm-like Bodies in Dioscorea opposita[J]. Chinese Bulletin of Botany, 2018, 53(3): 334-340.
Figure 1 Induction and regeneration of Dioscorea opposita cv. ‘Tiegun’ protocorm-like bodies (PLBs)(A) Explants; (B) Induction of PLBs; (C) Proliferation of PLBs; (D) Differentiation of PLBs; (E) Plants regenerated from PLBs; (F) Plants produced from PLBs after being sown in soil
Figure 2 Influence of TDZ concentration on the induction rate (A) and the induction number (B) of Dioscorea opposita cv. ‘Tiegun’ protocorm-like bodies (PLBs)Different lowercase letters indicate significant differences at P< 0.05 among different treatments.
Concentration of 6-BA (mg·L-1) | Multiplication index | Differentiation rate (%) | The growth of PLBs |
---|---|---|---|
5 | 4.50±1.15 a | 50.00 | There was no PLB proliferation during the later stage. 2-3 buds were differentiated from the PLBs |
7 | 4.67±0.95 a | 33.33 | There was no PLB proliferation during the later stage. 4-5 buds were differentiated from the PLBs |
9 | 5.42±1.61 a | 8.33 | Light green PLBs with larger size and bud tips differentiated |
12 | 4.92±0.14 a | 8.33 | Pale green PLBs with larger size and significantly differentiated bud tips |
15 | 4.17±1.13 a | 8.33 | Green PLBs with smaller size and no differentiation |
Table 1 Influence of 6-BA concentration on the multiplication of Dioscorea opposita cv. ‘Tiegun' protocorm-like bodies (PLBs)
Concentration of 6-BA (mg·L-1) | Multiplication index | Differentiation rate (%) | The growth of PLBs |
---|---|---|---|
5 | 4.50±1.15 a | 50.00 | There was no PLB proliferation during the later stage. 2-3 buds were differentiated from the PLBs |
7 | 4.67±0.95 a | 33.33 | There was no PLB proliferation during the later stage. 4-5 buds were differentiated from the PLBs |
9 | 5.42±1.61 a | 8.33 | Light green PLBs with larger size and bud tips differentiated |
12 | 4.92±0.14 a | 8.33 | Pale green PLBs with larger size and significantly differentiated bud tips |
15 | 4.17±1.13 a | 8.33 | Green PLBs with smaller size and no differentiation |
Treatments | Differentiation rate (%) | Average plant height (cm) | The growth of plant |
---|---|---|---|
1 | 100 | 3.1±0.43 c | Plants with fewer branches and slightly unfolded leaves |
2 | 100 | 1.4±0.54 b | Plants with more branches, slightly unfolded leaves and horizontal stems |
3 | 100 | 5.0±1.13 a | Plants with the most branches, slightly unfolded leaves and vertical stems |
Table 2 Influence of culture mediums on the differentiation of Dioscorea opposita cv. ‘Tiegun’ protocorm-like bodies (PLBs) (means±SE)
Treatments | Differentiation rate (%) | Average plant height (cm) | The growth of plant |
---|---|---|---|
1 | 100 | 3.1±0.43 c | Plants with fewer branches and slightly unfolded leaves |
2 | 100 | 1.4±0.54 b | Plants with more branches, slightly unfolded leaves and horizontal stems |
3 | 100 | 5.0±1.13 a | Plants with the most branches, slightly unfolded leaves and vertical stems |
[1] | 毕玲, 刘凤栾, 董爱香, 周晓阳, 马男, 赵梁军 (2012). 狗蔷薇类原球茎遗传转化体系的建立. 核农学报 26, 270-274. |
[2] | 蔡建荣 (2008). 山药组织培养褐化反应的研究. 中国农学通报 24(8), 118-120. |
[3] | 陈钢, 林兰英, 王建勤 (1994). 药用金线莲组培类原球茎研究初报. 福建中医药 25(4), 23-24. |
[4] | 陈晓旋, 李洪波, 叶春海, 丰锋, 卢淑仪, 李柳菁 (2010). 文心兰类原球茎的诱导与增殖技术的优化. 热带作物学报 31, 1464-1468. |
[5] | 崔广荣, 侯喜林, 张子学, 张从宇, 胡能兵, 刘跃成 (2007). 蝴蝶兰叶片离体培养胚状体的发生及组织学观察. 园艺学报 34, 431-436. |
[6] | 崔广荣, 张子学, 张从宇, 胡能兵 (2009). 文心兰、蝴蝶兰类原球茎薄切片高频诱导PLBs. 安徽科技学院学报 23(2), 21-27. |
[7] | 古碧珠, 刘柏涛, 何嘉碧, 梁红 (2008). 石斛兰和秋花独蒜兰种子的原球茎诱导及其生长分化. 仲恺农业技术学院学报 21(4), 17-21. |
[8] | 韩林林, 李俊华, 赵喜亭, 张晓丽, 宋志辉, 刘世宇, 李明军 (2016). 农杆菌介导的怀山药叶片瞬时表达方法的建立. 河南师范大学学报(自然科学版) 44(6), 135-139. |
[9] | 韩晓勇, 闫瑞霞, 殷剑美, 张培通, 郭文琦, 李春宏 (2013). 铁棍山药组织培养快繁及试管珠芽离体再生体系研究. 西北植物学报 33, 2120-2125. |
[10] | 洪森荣, 李明军 (2006). 玻璃化法超低温保存怀山药种质的技术研究. 中草药 37, 1715-1718. |
[11] | 姜福星, 刘凤栾, 赵梁军 (2012). 植物类原球茎的研究与应用. 核农学报 26, 478-483. |
[12] | 江艳华, 李艾莲, 陈彩霞, 崔思然, 赵锋 (2013). 半夏类原球茎的诱导及其植株再生研究. 中国农学通报 29(19), 153-158. |
[13] | 李海兵, 周娜, 赵姣, 李翔, 冯秋妍, 赵喜亭, 李明军 (2010). 怀山药种质资源的包埋玻璃化超低温保存与植株再生. 植物学报 45, 379-383. |
[14] | 李纪强 (2013). 铁棍山药类原球茎诱导增殖及植株再生的研究. 硕士论文. 新乡: 河南师范大学. pp. 1-43. |
[15] | 李明军 (1995). 多效唑——一种优良的植物生长调节剂. 植物学通报 12(2), 27-31. |
[16] | 李明军 (2004). 怀山药组织培养及其应用. 北京: 科学出版社. pp. 5-160. |
[17] | 李明军, 陈明霞, 郭君丽, 洪森荣, 张晓丽, 徐鑫, 刘萍 (2004a). 生长调节物质和糖对怀山药微型块茎诱导形成的影响. 华北农学报 19, 69-72. |
[18] | 李明军, 李金亭, 朱命炜, 张嘉宝 (1999). 怀山药的离体繁殖. 中草药 30, 296-298. |
[19] | 李明军, 刘纪华, 张嘉宝, 施介村, 郭仲琛 (1997a). 多效唑(MET)在玉米组培中的作用. 作物学报 23, 759-761. |
[20] | 李明军, 刘萍, 张嘉宝 (2000a). 怀山药微型块茎的离体诱导. 植物生理学通讯 36, 41-42. |
[21] | 李明军, 刘欣英, 李萍, 张晓丽, 赵喜亭, 柳俊, 谢从华 (2008). 山药微型块茎诱导形成的影响因子研究. 中草药 39, 905-910. |
[22] | 李明军, 薛建平, 陈明霞, 郭闽英, 邱卫花, 张嘉宝 (2000b). 不同因子对山药愈伤组织诱导的影响. 广西植物 20, 156-160. |
[23] | 李明军, 杨建伟, 张嘉宝 (1997b). 怀山药的茎段培养和快速繁殖. 植物生理学报 33, 275-276. |
[24] | 李明军, 张宝华, 王天亮, 张峰, 陈明霞, 张嘉宝 (2004b). 怀山药脱毒快繁技术研究. 见:陈振光. 全国植物组织培养、脱毒快繁及工厂化生产学术研讨会. 北京: 中国农业科技出版社. pp. 92-97. |
[25] | 李明军, 张峰, 陈明霞, 于相丽 (2003). 怀山药病毒病的研究. 中草药 34, 附3-附5. |
[26] | 李青 (2016). 绿色食品铁棍山药栽培技术. 河南农业 11, 31-32. |
[27] | 林江波, 王伟英, 李海明, 吴水金, 邹晖, 李跃森, 戴艺民 (2016). 铁皮石斛茎段原球茎的诱导、分化与植株再生. 福建农业学报 31, 1075-1079. |
[28] | 刘雯 (2014). 铁棍山药微型块茎形成的形态解剖及生理生化变化研究. 硕士论文. 新乡: 河南师范大学. pp. 1-34. |
[29] | 刘晓芳, 黄闽敏, 姜敏, 牛俊丽 (2009). 离体培养春兰原球茎增殖的影响因子研究. 西北林学院学报 24(3), 88-90. |
[30] | 罗剑飘, 吴坤林, 翁殊斐 (2015). 麒麟火焰兰叶片及根类原球茎的诱导研究. 浙江农业学报 27, 1173-1177. |
[31] | 马林, 张玲, 李卫锋 (2004). 黄山药丛生芽诱导与植株快速繁殖. 生物技术 14(2), 53-54. |
[32] | 彭晓英, 周朴华, 张良波, 彭尽晖, 周双德 (2010). 盾叶薯蓣类原球茎的离体诱导及快繁体系的建立. 植物遗传资源学报 11, 629-634. |
[33] | 乔永旭, 张永平, 陈超, 韩兴辉, 王旭, 刘桂萍 (2010). 蝴蝶兰组培苗类原球茎的诱导与分化. 北方园艺 (4), 138-141. |
[34] | 王丽萍, 梁淑云 (2010). 铁皮石斛原球茎诱导与增殖研究. 中国农学通报 26, 265-268. |
[35] | 许云, 高艳强, 高洪昌, 夏赟, 吴文嫱, 黄东益 (2014). 大薯类原球茎的离体诱导及再生体系的建立. 植物生理学报 50, 1027-1032. |
[36] | 杨美纯, 周歧伟, 许鸿源, 卢美英 (2000). 外部因子对蝴蝶兰叶片原球茎状体发生的影响. 广西植物 20, 42-46. |
[37] | 于倩, 李明军 (2004). 怀山药微型块茎愈伤组织的诱导形成及高频率再生. 生态学报 24, 1022-1026. |
[38] | 詹忠根, 徐程, 张铭, 罗紫娟 (2005). 铁皮石斛离体根尖经体细胞胚再生植株研究. 浙江大学学报(农业与生命科学版) 31, 579-580. |
[39] | 张超, 荣松, 张茜茜, 楼楠男 (2010). 基本培养基、碳源及植物生长调节剂对文心兰原球茎增殖的影响. 浙江农业科学 1, 55-59. |
[40] | 张瑞姿 (2012). 植物生长调节剂在观赏植物组织培养中的应用. 园林绿化 (5), 39-40. |
[41] | 张晓丽, 王医鹏, 刘雯, 郭晓博, 李书杰, 李明军 (2013). 铁棍山药试管苗快繁培养基的优化. 河南师范大学学报(自然科学版) 41, 123-126. |
[42] | 张玉, 李艳敏, 张和臣, 王利民, 王慧娟, 张鸽香 (2012). 蝴蝶兰叶片诱导类原球茎初探. 河南农业科学 41, 126-128. |
[43] | 张振霞, 叶静鹏, 郑莉, 陈贵豪, 郑玉忠 (2016). 广山药组织培养技术的研究. 江苏农业科学 44(2), 76-80. |
[44] | 赵喜亭, 王苗, 邵换娟, 李翔, 李海兵, 李明军 (2012). 山药种质包埋玻璃化超低温保存再生植株的稳定性分析. 华北农学报 27, 234-238. |
[45] | Li MJ, Li JH, Liu W, Liu LY, Lu J, Niu J, Liu XY, Yang QX (2014). A protocol for in vitro production of microtubers in Chinese yam ( Dioscorea opposita). Biosci Biotechnol Bio- chem 78, 1005-1009. |
[46] | Li MJ, Zhao XT, Hong SR, Zhang XL, Li P, Liu J, Xie CH (2009). Cryopreservation of plantlet nodes of Dioscorea opposita Thunb. using a vitrification method. CryoLetters 30, 19-28. |
[47] | Tian CW, Chen Y, Zhao XL, Zhao LJ (2008). Plant regene- ration through protocorm-like bodies induced from rhi- zoids using leaf explants of Rosa spp. Plant Cell Rep 27, 823-831. |
[1] | . Establishment of Tissue Culture and Rapid System of Erythropalum scandens Based on Orthogonal Test [J]. Chinese Bulletin of Botany, 2024, 59(1): 0-0. |
[2] | Liu Xiaofei, Sun Yingbo, Huang Lili, Yang Yuchai, Zhu Genfa, Yu Bo. Efficient Plant Regeneration via Somatic Embryogenesis in Alocasia reginula cv. ‘Black Velvet’ [J]. Chinese Bulletin of Botany, 2023, 58(5): 750-759. |
[3] | Jiming Cheng, Huimin He, Hongyu Niu, Hongmao Zhang. Research progress on the effect of intraspecific personality differences on seed dispersal in rodents [J]. Biodiv Sci, 2023, 31(4): 22446-. |
[4] | ZHANG Jin-Yan, CUN Zhu, SHUANG Sheng-Pu, HONG Jie, MENG Zhen-Gui, CHEN Jun-Wen. Steady-state and dynamic photosynthetic characteristics of shade-tolerant species Panax notoginseng in response to nitrogen levels [J]. Chin J Plant Ecol, 2023, 47(3): 331-347. |
[5] | Lulu Ren, Youze Zhang, Kelin Huang, Xiaochun Wan, Zhaoliang Zhang, Mulan Zhu, Chaoling Wei. An Efficient System for Regenerating Adventitious Buds in Stem Segments of Tea Plants [J]. Chinese Bulletin of Botany, 2023, 58(2): 308-315. |
[6] | Jinchun Lu, Lina Cao, Guanjie Tong, Xinying Wang, Liying Zhang, Xin Yu, Huifang Li, Yanhui Li. Establishment of Callus Induction and Regeneration System of Anemone silvestris [J]. Chinese Bulletin of Botany, 2022, 57(2): 217-226. |
[7] | Mengyue Li, Liu Liu, Yan Liu, Xiaoman Zhang. Establishment of Tissue Culture System for Axillary Bud Regeneration of Primula × pubescens [J]. Chinese Bulletin of Botany, 2021, 56(6): 732-739. |
[8] | Yaqian Xiong, Xianbao Deng, Huihui Zhang, Dong Yang, Heng Sun, Juan Liu, Mei Yang. In Vitro Rapid Propagation of Nelumbo nucifera [J]. Chinese Bulletin of Botany, 2021, 56(5): 605-613. |
[9] | Qian Luo, Yansha Zhang, Jing Ou. Callus Induction and Plant Regeneration of Cerasus serrulata var. lannesiana cv. ‘Grandiflora’ [J]. Chinese Bulletin of Botany, 2021, 56(4): 451-461. |
[10] | Xifu Yang, Hongmao Zhang, Zhibin Zhang. Mast seeding and its relationship to animal hoarding behaviour [J]. Biodiv Sci, 2020, 28(7): 821-832. |
[11] | Yan Xiao,Zhenxing Wang,Dongming Li,Yanhua Qi, Enhebayaer. Optimization of Tissue Culture and Plant Regeneration System of Mature Embryo of Leymus chinensis [J]. Chinese Bulletin of Botany, 2020, 55(2): 192-198. |
[12] | Xianjun Lai,Yizheng Zhang,Yinghong Gu,Lang Yan. Transformation of Insect Derived Antifreeze Gene into Sweet Potato (Ipomoea batatas) and Enhanced Its Freeze-tolerance [J]. Chinese Bulletin of Botany, 2020, 55(1): 9-20. |
[13] | Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu. Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum [J]. Chinese Bulletin of Botany, 2019, 54(6): 773-778. |
[14] | Ying Feng,Lianwen Qian,Qingliang Lin. The Effect of Different Hormones on Explant Browning and Callus Browning in Cyclocarya paliurus [J]. Chinese Bulletin of Botany, 2019, 54(5): 634-641. |
[15] | Yue Xu,Yingping Cao,Yu Wang,Chunxiang Fu,Shaojun Dai. Agrobacterium rhizogenes-mediated Transformation System of Spinacia oleracea [J]. Chinese Bulletin of Botany, 2019, 54(4): 515-521. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||