Chinese Bulletin of Botany ›› 2024, Vol. 59 ›› Issue (3): 441-451.DOI: 10.11983/CBB23105  cstr: 32102.14.CBB23105

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of a Fast Breeding System for Itoh Hybrid ‘He Xie’ in Tissue Culture

Min Kang1,4, Meiying Zhang1,4, Xiushuang Qi6, Ningning Tong4,5, Yang Li4,5, Qingyan Shu4,5, Zheng’an Liu4,5, Changping Lü2,3,*(), Liping Peng4,5,*()   

  1. 1College of Horticulture, Hunan Agricultural University, Changsha 410128, China
    2College of Landscape Architecture and Art Design, Hunan Agricultural University, Changsha 410128, China
    3Hunan Provincial Engineering and Technology Research Center for Breeding and Utilization of Central-subtropical High-quality Flowering Trees, Hunan Agricultural University, Changsha 410128, China
    4National Key Laboratory of Plant Diversity and Special Economic Crops, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
    5National Botanical Garden, Beijing 100093, China
    6Tian Xiang Yuan (Liaoning) Biotechnology Co. Ltd., Huludao 125300, China
  • Received:2023-08-03 Accepted:2023-12-19 Online:2024-05-10 Published:2024-05-10
  • Contact: E-mail: changpinglv@sina.com; pengliping@ibcas.ac.cn

Abstract: An in vitro rapid propagation system using scale buds of Itoh hybrids ‘He Xie’ can overcome the shortcomings of the slow traditional breeding methods and promote the adoption of the excellent Itoh hybrid varieties. In this study, we used the buds of ‘He Xie’ as explants to investigate the effects of various factors including disinfection time, plant growth regulators (PGRs) concentration and root induction time, and different rooted seedling grades on the initiation, proliferation, rooting and domestication of ‘He Xie’ with one-way experimental design. Our results showed: the optimal disinfection time of buds by 2% sodium hypochlorite solution was 12 min, with a contamination rate of 9.09%; the optimal initial culture medium was MS+1.5 mg∙L-1 6-BA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3; the optimal proliferation culture medium was MS+450 mg∙L-1 CaCl2+0.5 mg∙L-1 6-BA+0.2 mg∙L-1 IBA+0.2 mg∙L-1 GA3+0.5 mg∙L-1 AgNO3 with a proliferation rate of 3.3. Rootless seedlings were cultured on root induction medium 1/2MS+1.0 mg∙L-1 putrescine+2.0 mg∙L-1 IBA for 8 d at 4°C in dark and then 30 d at room temperature under light, and finally transferred to root formation medium 1/2MS+1.0 g∙L-1 AC for 20 d, a rooting rate of 66.7% was observed. The rooted seedlings were transplanted on a growing matrix of perlite:vermiculite:charcoal soil=1:1:1 (v/v/v) for 60 d, with the highest transplant survival rate of 52.0% being observed for first-grade rooted seedlings, but most of the second- and third-grade seedlings being dead, suggesting that the quality of rooting is critical for transplant survival.

Key words: Itoh ‘He Xie’, initiation and proliferation, rooting, scale buds, transplant domestication