Chinese Bulletin of Botany ›› 2018, Vol. 53 ›› Issue (3): 341-352.DOI: 10.11983/CBB17103

Special Issue: 药用植物专辑

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Ultrasound-microwave-assisted Extraction of Alkaloids from Epimedium brevicornum and Their Inhibitory Effect on HeLa Cells

Li Lu1, An Yejuan1, Qiao Chunlei1, Yang Xiaohua2,*(), Zhang Huafeng1,*(), Xue Mengying1   

  1. 1International Joint Research Center of Shaanxi Province for Food and Health Sciences, National Engineering Laboratory for Resources Development of Endangered Crude Drugs in Northwest China, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China;
    2Key Laboratory of Ministry of Health for Forensic Sciences, School of Medicine, Xi'an Jiaotong University, Xi’an 710061, China;
  • Received:2017-05-23 Accepted:2017-08-30 Online:2018-05-01 Published:2018-09-11
  • Contact: Yang Xiaohua,Zhang Huafeng

Abstract:

A protocol of ultrasound-microwave-assisted extraction was developed to isolate alkaloids from the medicinal plant Epimedium brevicornum, and the extraction mechanism is discussed. The chemical composition of the alkaloid extract was analyzed. The inhibitory effect of E. brevicornum alkaloids on human cervical cancer HeLa cells was investigated. The optimal condition for ultrasound-microwave-assisted extraction of E. brevicornum alkaloids was achieved by using an orthogonal test: steeped time, 40 min; ultrasound-microwave time, 18 min; microwave power, 250 J∙s-1; liquid-to-solid ratio, 30 mL∙g-1; and ethanol concentration, 70%. Extraction yield of alkaloids by ultrasound- microwave-assisted extraction reached 16.146 mg∙g-1, which was significantly higher than that by ultrasound-assisted extraction, microwave-assisted extraction and heating extraction. Scanning electron micrographs showed that the synergism between the ultrasound and microwave resulted in many interstices on the surface of leaf samples of E. brevicornum. Laser diffraction particle sizing analysis revealed that the amount of small and medium particles of leaf samples increased and that of large particles decreased after ultrasound-microwave-assisted extraction. Thin-layer chromatography and high-performance liquid chromatography revealed that the alkaloid extract contained magnoflorine, and berberine was not detectable. E. brevicornum alkaloids exhibited a significant dose-dependent inhibitory activity against human cervical cancer HeLa cells. The present research may be helpful for the exploitation and utilization of Epimedium resources.

Key words: Epimedium brevicornum, alkaloids, ultrasound-microwave-assisted extraction, mechanism, HeLa cells