Chinese Bulletin of Botany ›› 2017, Vol. 52 ›› Issue (6): 764-773.DOI: 10.11983/CBB16233

• TECHNIQUES AND METHODS • Previous Articles     Next Articles

Establishment of a Tissue Culture and Propagation System for Ardisia violacea, a Rare and Endangered Species

Sun Yingkun1, Hu Shaoqing2, Pang Jiliang3, Gao Kai1, Liu Huahong1, Chen Huanwei4, Yao Tao1, Chen Linjing1, Shen Bochun1,*()   

  1. 1Hangzhou Landscaping Incorporated, Hangzhou 310000, China
    2College of Civil Engineering and Architecture, Zhejiang Sci-Tech University, Hangzhou 310018, China
    3College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
    4Longquan Forestry Research Institute, Longquan 323700, China
  • Received:2016-11-30 Accepted:2017-05-04 Online:2017-11-01 Published:2018-02-22
  • Contact: Shen Bochun

Abstract: Shoot segments with axillary buds from young branches of wild Ardisia violacea with excellent characters were cultured in vitro. Through bud induction, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0.80 mg?L-1 KT+0.10 mg?L-1 NAA+0.10 mg?L-1 IBA, and the germination frequency of axillary buds reached 92.60%; the optimal multiple shoot proliferation medium was MS1+0.50 mg?L-1 TDZ+0.10 mg?L-1 NAA, and the average proliferation coefficient reached 8.60; the optimal subculture medium was MS+1.00 mg?L-1 KT+0.50 mg?L-1 NAA; the optimal rooting medium was 1/2MS+2.00 mg?L-1 IBA+1.00 mg?L-1 NAA+1.00 mg?L-1 AC, and the average frequency of shoot rooting reached 98.70%. Using pine bark:peat (2:1, v/v) as a matrix, the transplant survival rate could reach 85.30%. We established a high-frequency regeneration system for A. violacea that could meet the needs of large-scale production.

Key words: Ardisia violacea, bud induction, multiple shoots