Rapid Propagation of Symplocos paniculata In Vitro

doi:10.11983/CBB18034

Abstract

Abstract: With tender stem segments of Symplocos paniculata as the experiment material, the tissue culture system of S. paniculata was preliminarily established to research the key factors influencing initiation culture, proliferation, rooting culture and transplantation. Treatment with 0.1% HgCl₂ for 3 min was the optimum scheme for explant sterilization of S. paniculata; survival rate of sterile seedlings was 81%. The rate of germination of initiation culture was 86.83% in the medium with 1/2MS+30 g∙L^-1 sucrose+8 g∙L^-1 agar. The proliferation coefficient was 3.57 in optimum culture medium with 1/2MS+1.0 mg∙L^-1 6-BA+0.02 mg∙L^-1 IBA+30 g∙L^-1 sucrose+8 g∙L^-1 agar. The suitable rooting medium was WPM+0.5 mg∙L^-1 IBA+0.5 mg∙L^-1 NAA+20 g∙L^-1 sucrose+2 g∙L^-1 AC+8 g∙L^-1agar, and rooting rate reached 93%. After being acclimatized, the regenerated plants were transplanted into substrate of garden soil and peat soil (v:v=1:1), with survival rate of regenerated plants of 83%.

Key words: Symplocos paniculata, explant, tissue culture

An Baiyi, Guo Cainan, Bao Wenhui, Li Fengfei, Zhao He, Chen Li, An Fengyun. Rapid Propagation of Symplocos paniculata In Vitro[J]. Chinese Bulletin of Botany, 2018, 53(5): 693-699.

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URL: https://www.chinbullbotany.com/EN/10.11983/CBB18034

https://www.chinbullbotany.com/EN/Y2018/V53/I5/693

Figures/Tables 6

References 17

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Sterilization time (min)	1	2	3	4	5
Acquisition rate (%)	46.0	70.0	81.0	45.0	10.8
Pollution rate (%)	50.0	25.0	18.2	16.7	14.0

Sterilization time (min)	1	2	3	4	5
Acquisition rate (%)	46.0	70.0	81.0	45.0	10.8
Pollution rate (%)	50.0	25.0	18.2	16.7	14.0

Culture medium	Average germination rate (%)	State of growth
WPM	43.30±5.03 eE	Sprouts grew generally, leaves were normal shape and color
Read	56.37±4.05 dD	Sprouts grew generally, leaves were normal shape but yellow
MS	14.13±5.00 fF	Sprouts grew poorly, leaves were narrow, curly, green
mMS	16.00±5.00 fF	Sprouts grew poorly, leaves were curly, green
1/2MS	86.83±5.53 aA	Sprouts grew well, leaves were flat and dark green
1/2mMS	54.33±4.51 dD	Sprouts grew well, leaves were curly, dark green
1/4MS	74.13±3.61 bB	Sprouts grew generally, leaves were curly, green
1/8MS	66.33±3.21 cC	Sprouts grew generally, leaves were curly and yellow

Culture medium	Average germination rate (%)	State of growth
WPM	43.30±5.03 eE	Sprouts grew generally, leaves were normal shape and color
Read	56.37±4.05 dD	Sprouts grew generally, leaves were normal shape but yellow
MS	14.13±5.00 fF	Sprouts grew poorly, leaves were narrow, curly, green
mMS	16.00±5.00 fF	Sprouts grew poorly, leaves were curly, green
1/2MS	86.83±5.53 aA	Sprouts grew well, leaves were flat and dark green
1/2mMS	54.33±4.51 dD	Sprouts grew well, leaves were curly, dark green
1/4MS	74.13±3.61 bB	Sprouts grew generally, leaves were curly, green
1/8MS	66.33±3.21 cC	Sprouts grew generally, leaves were curly and yellow

6-BA (mg∙L^-1)	IBA (mg∙L^-1)	Average proliferation coefficient	State of growth
0.06	0	1.33±0.21 kJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were weak
0.06	0.02	1.40±0.00 kIJ	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were weak
0.06	0.05	1.47±0.15 kIJ	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were weak
0.06	0.08	1.67±0.21 ijkHIJ	Sprouted within 16 days in average, base of seedlings had many calli, lateral buds were weak
0.3	0	1.63±0.23 jkHIJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were slender
0.3	0.02	1.57±0.12 jkHIJ	Sprouted within 22 days in average, base of seedlings had few calli, lateral buds were thick
0.3	0.05	1.57±0.29 jkHIJ	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were thick
0.3	0.08	1.90±0.20 hijGHI	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were thick
0.3	0.2	2.43±0.12 fgDEF	Sprouted within 9 days in average, base of seedlings had few calli, lateral buds were brawny
0.3	0.5	1.70±0.17 ijkHIJ	Sprouted within 15 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0	2.77±0.25 defCD	Sprouted within 13 days in average, base of seedlings had few calli, lateral buds were slender
0.6	0.02	2.03±0.06 hiFGH	Sprouted within 45 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.05	3.50±0.26 abAB	Sprouted within 13 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.08	2.93±0.15 cdCD	Sprouted within 16 days in average, base of seedlings had many calli, lateral buds were thick
0.6	0.2	2.60±0.20 defDE	Sprouted within 15 days in average, base of seedlings had very few calli, lateral buds were thick
0.6	0.5	3.17±0.29 bcBC	Sprouted within 15 days in average, base of seedlings had few calli, lateral buds were brawny
0.6	0.8	2.20±0.27 ghEFG	Sprouted within 13 days in average, base of seedlings had many calli, lateral buds were brawny
1.0	0	2.70±0.20 defCD	Sprouted within 12 days in average, base of seedlings had many calli, lateral buds were brawny
1.0	0.02	3.57±0.32 aAB	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.05	1.67±0.12 ijkHIJ	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.08	3.50±0.20 abAB	Sprouted within 14 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.2	2.53±0.25 defgDE	Sprouted within 8 days in average, base of seedlings had very few calli, lateral buds were brawny
1.0	0.5	2.90±0.17 cdeCD	Sprouted within 9 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	0.8	3.87±0.06 aA	Sprouted within 5 days in average, base of seedlings had few calli, lateral buds were brawny
1.0	1.1	2.93±0.32 cdCD	Sprouted within 4 days in average, base of seedlings had a great many calli, lateral buds were brawny